The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Glutathione synthesis is essential for mouse development but not for cell growth in culture
Open accessAbstract
Glutathione (GSH) is a major source of reducing equivalents in mammalian cells. To examine the role of GSH synthesis in development and cell growth, we generated mice deficient in GSH by a targeted disruption of the heavy subunit of γ-glutamylcysteine synthetase (γGCS-HS tm1), an essential enzyme in GSH synthesis. Embryos homozygous for γGCS-HS tm1 fail to gastrulate, do not form mesoderm, develop distal apoptosis, and die before day 8.5. Lethality results from apoptotic cell death rather than reduced cell proliferation. We also isolated cell lines from homozygous mutant blastocysts in medium containing GSH. These cells also grow indefinitely in GSH-free medium supplemented with N-acetylcysteine and have undetectable levels of GSH; further, they show no changes in mitochondrial morphology as judged by electron microscopy. These data demonstrate that GSH is required for mammalian development but dispensable in cell culture and that the functions of GSH, not GSH itself, are essential for cell growth.
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Lipid peroxidation and ferroptosis: The role of GSH and GPx4
Ursini, F., & Maiorino, M. (2020) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2020.02.027 · Cited by 1,954
Seiler, A., Schneider, M., Förster, H., Roth, S., Wirth, E. K., Culmsee, C., Plesnila, N., Kremmer, E., Rådmark, O., Wurst, W., et al. (2008) · Cell Metabolism · doi:10.1016/j.cmet.2008.07.005 · Cited by 1,470
Triggering and modulation of apoptosis by oxidative stress
Chandra, J., Samali, A., & Orrenius, S. (2000) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(00)00302-6 · Cited by 1,335
Kim, Y.-K., Na, K.-S., Myint, A.-M., & Leonard, B. E. (2016) · Progress in Neuro-Psychopharmacology and Biological Psychiatry · doi:10.1016/j.pnpbp.2015.06.008 · Cited by 620
Franklin, C. C., Backos, D. S., Mohar, I., White, C. C., Forman, H. J., & Kavanagh, T. J. (2009) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2008.08.009 · Cited by 438
Metadata
- Authors
- Shi, Z.-Z., Osei-Frimpong, J., Kala, G., Kala, S. V., Barrios, R. J., Habib, G. M., Lukin, D. J., Danney, C. M., Matzuk, M. M., & Lieberman, M. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-05-09
- Volume
- 97
- Issue
- 10
- Pages
- 5101-5106
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2034005931
- Cited by
- 295
- References
- 40
- FWCI
- 5.97
- Type
- journal article
- Language
- en
- Open access
- green
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- Shi, Z.-Z., Osei-Frimpong, J., Kala, G., Kala, S. V., Barrios, R. J., Habib, G. M., Lukin, D. J., Danney, C. M., Matzuk, M. M., & Lieberman, M. W. (2000). Glutathione synthesis is essential for mouse development but not for cell growth in culture. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.97.10.5101
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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