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C-terminal Extension of Truncated Recombinant Proteins in Escherichia coli with a 10Sa RNA Decapeptide
Open accessAbstract
When murine interleukin-6 is overexpressed in Escherichia coli, a small population of molecules exhibits a novel C-terminal modification. Peptide mapping, electrospray ionization-mass spectrometry, and automated N- and C-terminal sequencing identified a peptide ("tag" peptide), -Ala-Ala-Asn-Asp-Glu-Asn-Tyr-Ala-Leu-Ala-Ala-COOH, encoded by a small metabolically stable RNA of E. coli (10Sa RNA) attached to truncated C termini of the recombinant protein. A mutant strain of E. coli in which the chromosomal 10Sa RNA gene (ssrA) is disrupted does not produce this C-terminal modification, confirming that the tag peptide originates from the ssrA gene.
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Cited by
Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA
Keiler, K. C., Waller, P. R. H., & Sauer, R. T. (1996) · Science · doi:10.1126/science.271.5251.990 · Cited by 1,125
Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis
Flynn, J. M., Levchenko, I., Seidel, M., Wickner, S. H., Sauer, R. T., & Baker, T. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.191375298 · Cited by 334
Programmed translational frameshifting
Farabaugh, P. J. (1996) · Microbiological Reviews · doi:10.1128/mr.60.1.103-134.1996 · Cited by 316
The bacterial translation stress response.
Starosta, A. L., Lassak, J., Jung, K., & Wilson, D. N. (2014) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12083 · Cited by 210
tRNA properties help shape codon pair preferences in open reading frames
Buchan, J. R. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkj488 · Cited by 108
Metadata
- Authors
- Tu, G.-F., Reid, G. E., Zhang, J.-G., Moritz, R. L., & Simpson, R. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-04
- Volume
- 270
- Issue
- 16
- Pages
- 9322-9326
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2028161058
- Cited by
- 235
- References
- 0
- FWCI
- 4.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Tu, G.-F., Reid, G. E., Zhang, J.-G., Moritz, R. L., & Simpson, R. J. (1995). C-terminal Extension of Truncated Recombinant Proteins in Escherichia coli with a 10Sa RNA Decapeptide. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.270.16.9322
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