Cited by 1,125RNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyRNA Research and Splicing

Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA

Keiler, Kenneth C., Waller, Patrick R. H., Sauer, Robert T.·1996·Science

Abstract

Variants of lambda repressor and cytochrome b562 translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli. The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.

Integrity signals

Registry

Crossref · 10.1126/science.271.5251.990

Cites

28 of 28 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The N-end rule and the SsrA tag are the best characterized mechanisms by which bacterial proteases recognize substrates.

    “Currently, the most well characterized mechanisms for substrate recognition by bacterial proteases are the N-end rule and the SsrA tag.”
  • The SsrA tag is an eleven-amino-acid tag added to the carboxy-terminus of incompletely translated proteins and marks them for degradation.

    “The N-end rule relates the half-life of a protein to the N-terminal amino acid while the SsrA tag is an eleven amino acid tag that is added to the carboxy-terminus of incompletely translated proteins relegating them to degradation [54–55].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Keiler, K. C., Waller, P. R. H., & Sauer, R. T.
Affiliations
Massachusetts Institute of Technology (US)
Corresponding author
Sauer, Robert T.
Venue
Science
Published
1996-02-16
Volume
271
Issue
5251
Pages
990-993
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2084140927
Cited by
1,125
References
28
Citations by year
2026: 27 · 2025: 24 · 2024: 34 · 2023: 22 · 2022: 33 · 2021: 36 · 2020: 36 · 2019: 27 · 2018: 26 · 2017: 39 · 2016: 27 · 2015: 24 · 2014: 42 · 2013: 31 · 2012: 34
FWCI
15.80
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Allergy and Infectious Diseases (AI15706, R01AI016892, AI‑16892)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
ssrA, ribosome stalling, protein degradation, lambda repressor, cytochrome b562, Escherichia coli
MeSH terms
Alanine, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Cytochrome b Group, DNA-Binding Proteins, Escherichia coli, Molecular Sequence Data, Endopeptidases, Peptides, Protein Processing, Post-Translational, Repressor Proteins, RNA, Bacterial, RNA, Messenger, Protein Biosynthesis, Viral Proteins, Codon, Terminator, Escherichia coli Proteins, Viral Regulatory and Accessory Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review