The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Both v-Ha-Ras and v-Raf Stimulate Expression of the Vascular Endothelial Growth Factor in NIH 3T3 Cells
Open accessAbstract
Stimulation of NIH 3T3 cells with platelet-derived growth factor (PDGF)-BB and 12-O-tetradecanoylphorbol-13-acetate (TPA) enhances vascular endothelial growth factor (VEGF) gene expression. To address the question of whether Ras and Raf are involved in the induction of VEGF gene expression by PDGF and TPA, we examined the effects of both factors on NIH 3T3 cells stably transfected with v-Ha-ras or v-raf. In serum-starved NIH 3T3 cells, only low levels of mRNA expression can be detected, whereas both ras and raf transformed cell lines express enhanced levels of a 4.3-kilobase VEGF transcript. Stimulation with PDGF or TPA resulted in increased VEGF mRNA in all cell lines, with highest levels found in the transformed cells. Immunofluorescence studies confirmed that the elevated VEGF mRNA expression correlated with enhanced protein levels. Positive immunofluorescence signals could be detected in v-Ha-ras or v-raf transformed cell lines but not in unstimulated NIH 3T3 cells. VEGF from conditioned medium of v-raf transformed NIH 3T3 cells was partially purified by chromatography on heparin-Sepharose. Biological activity of this VEGF protein was demonstrated by competition with binding of recombinant 125I-VEGF165 to human umbilical vein endothelial cells and by its ability to stimulate proliferation of these cells.
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The biology of VEGF and its receptors
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The splice variants of vascular endothelial growth factor (VEGF) and their receptors
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Metadata
- Authors
- Grugel, S., Finkenzeller, G., Weindel, K., Barleon, B., & Marmé, D.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-10
- Volume
- 270
- Issue
- 43
- Pages
- 25915-25919
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2019273955
- Cited by
- 329
- References
- 38
- FWCI
- 5.80
- Type
- journal article
- Language
- en
- Open access
- hybrid
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