The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
The Effects of Wortmannin on Rat Skeletal Muscle
Open accessAbstract
Both the anabolic hormone insulin and contractile activity stimulate the uptake of glucose into mammalian skeletal muscle. In this study, we examined the role of phosphatidylinositol 3-kinase (PI 3-kinase), a putative mediator of insulin actions, in the stimulation of hexose uptake in response to hormone and contraction. Phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-triphosphate accumulate in skeletal muscle exposed to insulin but not hypoxia, which mimics stimulation of the contractile-dependent pathway of hexose transport activation. The fungal metabolite wortmannin, an inhibitor of PI 3-kinase, completely blocks the appearance of 3'-phospholipids in response to insulin. Moreover, wortmannin entirely prevented the increase in hexose uptake in muscle exposed to insulin but was without effect on muscle stimulated by repetitive contraction or hypoxia. These results support the view that PI 3-kinase is involved in the signaling pathways mediating insulin-responsive glucose transport in skeletal muscle but is not required for stimulation by hypoxia or contraction. Furthermore, these data indicate that there exist at least two signaling pathways leading to activation of glucose transport in skeletal muscle with differential sensitivities to wortmannin.
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Oncogenes and signal transduction
Cantley, L. C., Auger, K. R., Carpenter, C., Duckworth, B., Graziani, A., Kapeller, R., & Soltoff, S. (1991) · Cell · doi:10.1016/0092-8674(91)90639-g · Cited by 2,694
White, M. F., & Kahn, C. R. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42297-6 · Cited by 1,723
Cheatham, B., Vlahos, C. J., Cheatham, L., Wang, L., Blenis, J., & Kahn, C. R. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.7.4902 · Cited by 1,068
Whitman, M., Downes, C. P., Keeler, M., Keller, T., & Cantley, L. (1988) · Nature · doi:10.1038/332644a0 · Cited by 1,016
Okada, T., Kawano, Y., Sakakibara, T., Hazeki, O., & Ui, M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)41901-6 · Cited by 918
Phosphatidylinositol 3-Kinase Encoded by Yeast VPS 34 Gene Essential for Protein Sorting
Schu, P. V., Takegawa, K., Fry, M. J., Stack, J. H., Waterfield, M. D., & Emr, S. D. (1993) · Science · doi:10.1126/science.8385367 · Cited by 778
Activation of phosphatidylinositol 3-kinase by insulin.
Ruderman, N. B., Kapeller, R., White, M. F., & Cantley, L. C. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.4.1411 · Cited by 524
Douen, A. G., Ramlal, T., Rastogi, S., Bilan, P. J., Cartee, G. D., Vranic, M., Holloszy, J. O., & Klip, A. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)77362-6 · Cited by 473
Dhand, R., Hiles, I., Panayotou, G., Roche, S., Fry, M. J., Gout, I., Totty, N. F., Truong, O., Vicendo, P., & Yonezawa, K. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06290.x · Cited by 446
Clarke, J. F., Young, P. W., Yonezawa, K., Kasuga, M., & Holman, G. D. (1994) · Biochemical Journal · doi:10.1042/bj3000631 · Cited by 358
Cited by
Exercise, Glucose Transport, and Insulin Sensitivity
Goodyear, L. J., & Kahn, B. B. (1998) · Annual Review of Medicine · doi:10.1146/annurev.med.49.1.235 · Cited by 1,056
Phosphoinositide 3-kinase: the key switch mechanism in insulin signalling
SHEPHERD, P. R., WITHERS, D. J., & SIDDLE, K. (1998) · Biochemical Journal · doi:10.1042/bj3330471 · Cited by 953
Mu, J., Brozinick, J. T., Valladares, O., Bucan, M., & Birnbaum, M. J. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00251-9 · Cited by 924
Self-citationMuscle‐derived interleukin‐6: mechanisms for activation and possible biological roles
FEBBRAIO, M. A., & PEDERSEN, B. K. (2002) · The FASEB Journal · doi:10.1096/fj.01-0876rev · Cited by 875
Exercise-induced increase in muscle insulin sensitivity
Holloszy, J. O. (2005) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00123.2005 · Cited by 494
Metadata
- Authors
- Yeh, J.-I., Gulve, E. A., Rameh, L., & Birnbaum, M. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-02
- Volume
- 270
- Issue
- 5
- Pages
- 2107-2111
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1525701947
- Cited by
- 314
- References
- 25
- FWCI
- 10.07
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Yeh, J.-I., Gulve, E. A., Rameh, L., & Birnbaum, M. J. (1995). The Effects of Wortmannin on Rat Skeletal Muscle. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.270.5.2107
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
