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Roles of a Membrane-localized βSubunit in the Formation and Targeting of Functional L-type Ca2+ Channels
Open accessAbstract
We report several unexpected findings that provide novel insights into the properties and interactions of the alpha 1 and beta subunits of dihydropyridine-sensitive L-type channels. First, the beta 2a subunit was expressed as multiple species of 68-72 kDa; the 70-72-kDa species arose from post-translational modification. Second, cell fractionation and immunocytochemical studies indicated that the hydrophilic beta 2a subunit, when expressed alone, was membrane-localized. Third, the beta 2a subunit increased the membrane localization of the alpha 1 subunit and the number of cells expressing L-type Ca2+ currents, without affecting the total amount of the expressed alpha 1C subunit. Expression of maximal currents in alpha 1C/beta 2a cotransfected cells paralleled the time course of expression of the beta subunit. Taken together, these results suggest that the beta subunit plays multiple roles in the formation, stabilization, targeting, and modulation of L-type channels.
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Cited by
Erickson, M. G., Alseikhan, B. A., Peterson, B. Z., & Yue, D. T. (2001) · Neuron · doi:10.1016/s0896-6273(01)00438-x · Cited by 465
Bichet, D., Cornet, V., Geib, S., Carlier, E., Volsen, S., Hoshi, T., Mori, Y., & De Waard, M. (2000) · Neuron · doi:10.1016/s0896-6273(00)80881-8 · Cited by 377
Phosphoinositides regulate ion channels
Hille, B., Dickson, E. J., Kruse, M., Vivas, O., & Suh, B.-C. (2015) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2014.09.010 · Cited by 312
Roden, D. M., Balser, J. R., George Jr, A. L., & Anderson, M. E. (2002) · Annual Review of Physiology · doi:10.1146/annurev.physiol.64.083101.145105 · Cited by 305
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Metadata
- Authors
- Chien, A. J., Zhao, X., Shirokov, R. E., Puri, T. S., Chang, C. F., Sun, D., Rios, E., & Hosey, M. M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-12
- Volume
- 270
- Issue
- 50
- Pages
- 30036-30044
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2063807479
- Cited by
- 269
- References
- 0
- FWCI
- 4.62
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Roles of a Membrane-localized βSubunit in the Formation and Targeting of Functional L-type Ca2+ Channels. https://app.cochat.ai/papers/10.1074/jbc.270.50.30036
- The paper
- Chien, A. J., Zhao, X., Shirokov, R. E., Puri, T. S., Chang, C. F., Sun, D., Rios, E., & Hosey, M. M. (1995). Roles of a Membrane-localized βSubunit in the Formation and Targeting of Functional L-type Ca2+ Channels. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.270.50.30036
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
