The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Adrenomedullin Expression in Human Tumor Cell Lines ITS POTENTIAL ROLE AS AN AUTOCRINE GROWTH FACTOR
Open accessAbstract
Although adrenomedullin (AM) previously has been identified in human tumors, its role has remained elusive. Analysis by reverse transcriptase-polymerase chain reaction (RT-PCR) revealed AM mRNA in 18 of 20 human normal tissues representing major organs, and 55 of 58 (95%) malignant cell lines. Western blot and high performance liquid chromatography analysis showed immunoreactive AM species of 18, 14, and 6 kDa that are consistent with the precursor, intermediate product, and active peptide, respectively. Immunohistochemistry and in situ RT-PCR performed on paraffin-embedded tumor cell lines of various tissue origins exhibited AM cytoplasmic staining. Neutralizing monoclonal antibody to AM inhibits tumor cell growth in a concentration-dependent manner, an effect that was reversed with the addition of exogenous AM. Responding tumor cells were shown to have approximately 50,000 AM receptors per cell by Scatchard analysis with 125I-AM and expressed AM receptor mRNA by RT-PCR. Our data showed 36 of 48 (75%) tumor cell lines expressed AM receptor mRNA by RT-PCR assessment, all of them also expressed AM. In the presence of AM, cAMP levels were shown to increase in tumor cells. Our collective data demonstrate that AM and AM receptor are expressed in numerous human cancer cell lines of diverse origin and constitute a potential autocrine growth mechanism that could drive neoplastic proliferation.
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Cites
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Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer
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Cited by
A Potential Role for Adrenomedullin as a Local Regulator of Bone Growth1
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Adrenomedullin is anti‐apoptotic in osteoblasts through CGRP1 receptors and MEK‐ERK pathway
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Metadata
- Authors
- Jean Miller, M., Martínez, A., Unsworth, E. J., Thiele, C. J., Moody, T. W., Elsasser, T., & Cuttitta, F.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-09
- Volume
- 271
- Issue
- 38
- Pages
- 23345-23351
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2071577747
- Cited by
- 314
- References
- 34
- FWCI
- 8.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Adrenomedullin Expression in Human Tumor Cell Lines ITS POTENTIAL ROLE AS AN AUTOCRINE GROWTH FACTOR. https://app.cochat.ai/papers/10.1074/jbc.271.38.23345
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- Jean Miller, M., Martínez, A., Unsworth, E. J., Thiele, C. J., Moody, T. W., Elsasser, T., & Cuttitta, F. (1996). Adrenomedullin Expression in Human Tumor Cell Lines ITS POTENTIAL ROLE AS AN AUTOCRINE GROWTH FACTOR. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.38.23345
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
