The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
The GTPase-activating Protein RGS4 Stabilizes the Transition State for Nucleotide Hydrolysis
Open accessAbstract
RGS proteins constitute a newly appreciated group of negative regulators of G protein signaling. Discovered by genetic screens in yeast, worms, and other organisms, two mammalian RGS proteins, RGS4 and GAIP, act as GTPase-activating proteins for members of the Gi family of G protein alpha subunits. We have purified recombinant RGS4 to homogeneity and demonstrate that it acts catalytically to stimulate GTP hydrolysis by Gi proteins. Furthermore, RGS4 stabilizes the transition state for GTP hydrolysis, as evidenced by its high affinity for the GDP-AlF4--bound forms of Goalpha and Gialpha and its relatively low affinity for the GTPgammaS- and GDP-bound forms of these proteins. Consequently, RGS4 is most likely not a downstream effector for activated Galpha subunits. All members of the Gi subfamily of proteins tested are substrates for RGS4 (including Gtalpha and Gzalpha); the protein has lower affinity for Gqalpha, and it does not stimulate the GTPase activity of Gsalpha or G12alpha.
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Cites
Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis
Coleman, D., Berghuis, A., Lee, E., Linder, M., Gilman, A., & Sprang (1994) · Science · doi:10.1126/science.8073283 · Cited by 825
Self-citationGAIP and RGS4 Are GTPase-Activating Proteins for the Gi Subfamily of G Protein α Subunits
Berman, D. M., Wilkie, T. M., & Gilman, A. G. (1996) · Cell · doi:10.1016/s0092-8674(00)80117-8 · Cited by 729
Self-citationCloning of bovine GAP and its interaction with oncogenic ras p21
Vogel, U. S., Dixon, R. A. F., Schaber, M. D., Diehl, R. E., Marshall, M. S., Scolnick, E. M., Sigal, I. S., & Gibbs, J. B. (1988) · Nature · doi:10.1038/335090a0 · Cited by 649
Koelle, M. R., & Horvitz, H. R. (1996) · Cell · doi:10.1016/s0092-8674(00)80998-8 · Cited by 569
GTPase mechanism of Gproteins from the 1.7-Å crystal structure of transducin α - GDP AIF−4
Sondek, J., Lambright, D. G., Noel, J. P., Hamm, H. E., & Sigler, P. B. (1994) · Nature · doi:10.1038/372276a0 · Cited by 558
Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family
Druey, K. M., Blumer, K. J., Kang, V. H., & Kehrl, J. H. (1996) · Nature · doi:10.1038/379742a0 · Cited by 468
Phospholipase C-β1 is a GTPase-activating protein for Gq/11, its physiologic regulator
Berstein, G., Blank, J. L., Jhon, D.-Y., Exton, J. H., Rhee, S. G., & Ross, E. M. (1992) · Cell · doi:10.1016/0092-8674(92)90165-9 · Cited by 427
Kozasa, T., & Gilman, A. G. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.4.1734 · Cited by 334
Self-citationRegulation of deactivation of photoreceptor G protein by its target enzyme and cGMP
Arshavsky, V. Y., & Bownds, M. D. (1992) · Nature · doi:10.1038/357416a0 · Cited by 322
Chan, R. K., & Otte, C. A. (1982) · Molecular and Cellular Biology · doi:10.1128/mcb.2.1.11-20.1982 · Cited by 319
Cited by
Ross, E. M., & Wilkie, T. M. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.795 · Cited by 1,156
Signal Transduction Cascades Regulating Fungal Development and Virulence
Lengeler, K. B., Davidson, R. C., D'souza, C., Harashima, T., Shen, W.-C., Wang, P., Pan, X., Waugh, M., & Heitman, J. (2000) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.64.4.746-785.2000 · Cited by 915
The Regulator of G Protein Signaling Family
De Vries, L., Zheng, B., Fischer, T., Elenko, E., & Farquhar, M. G. (2000) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.40.1.235 · Cited by 592
Disease-specific changes in regulator of G-protein signaling 4 (RGS4) expression in schizophrenia
Mirnics, K., Middleton, F. A., Stanwood, G. D., Lewis, D. A., & Levitt, P. (2001) · Molecular Psychiatry · doi:10.1038/sj.mp.4000866 · Cited by 446
Gold, S. J., Ni, Y. G., Dohlman, H. G., & Nestler, E. J. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-20-08024.1997 · Cited by 434
Metadata
- Authors
- Berman, D. M., Kozasa, T., & Gilman, A. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-11
- Volume
- 271
- Issue
- 44
- Pages
- 27209-27212
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2090636422
- Cited by
- 356
- References
- 23
- FWCI
- 9.52
- Type
- journal article
- Language
- en
- Open access
- hybrid
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