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Direct Association between the Yeast Rad51 and Rad54 Recombination Proteins

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Jiang, Hua, Xie, Yueqing, Houston, Peter, Stemke-Hale, Katherine, Mortensen, Uffe H., Rothstein, Rodney, Kodadek, Thomas·1996·Journal of Biological Chemistry

Abstract

The RAD54 and RAD51 genes are involved in genetic recombination and double-strand break repair in the yeast Saccharomyces cerevisiae. The Rad51 protein is thought to be a yeast analogue of the Eschericia coli recA gene product and catalyzes strand exchange between homologous single- and double-stranded DNAs in vitro. RAD54 exhibits homologies to several known ATPases and is a member of the SWI2/MOT1 family. We show here that the Rad54 protein interacts with the Rad51 protein in vivo and in vitro and that the NH2-terminal 115 residues of the Rad54 protein are necessary for this interaction. Combined with previously reported results, these data imply that the Rad54 protein is part of a multiprotein yeast recombination complex.

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Metadata

Authors
Jiang, H., Xie, Y., Houston, P., Stemke-Hale, K., Mortensen, U. H., Rothstein, R., & Kodadek, T.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1996-12
Volume
271
Issue
52
Pages
33181-33186
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2040015408
Cited by
158
References
0
FWCI
4.95
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Direct Association between the Yeast Rad51 and Rad54 Recombination Proteins. https://app.cochat.ai/papers/10.1074/jbc.271.52.33181
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Jiang, H., Xie, Y., Houston, P., Stemke-Hale, K., Mortensen, U. H., Rothstein, R., & Kodadek, T. (1996). Direct Association between the Yeast Rad51 and Rad54 Recombination Proteins. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.52.33181

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.