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Direct Association between the Yeast Rad51 and Rad54 Recombination Proteins
Open accessAbstract
The RAD54 and RAD51 genes are involved in genetic recombination and double-strand break repair in the yeast Saccharomyces cerevisiae. The Rad51 protein is thought to be a yeast analogue of the Eschericia coli recA gene product and catalyzes strand exchange between homologous single- and double-stranded DNAs in vitro. RAD54 exhibits homologies to several known ATPases and is a member of the SWI2/MOT1 family. We show here that the Rad54 protein interacts with the Rad51 protein in vivo and in vitro and that the NH2-terminal 115 residues of the Rad54 protein are necessary for this interaction. Combined with previously reported results, these data imply that the Rad54 protein is part of a multiprotein yeast recombination complex.
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Citations
Cited by
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Pâques, F., & Haber, J. E. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.2.349-404.1999 · Cited by 2,120
Choreography of the DNA Damage Response
Lisby, M., Barlow, J. H., Burgess, R. C., & Rothstein, R. (2004) · Cell · doi:10.1016/j.cell.2004.08.015 · Cited by 891
Self-citationGasch, A. P., Huang, M., Metzner, S., Botstein, D., Elledge, S. J., & Brown, P. O. (2001) · Molecular Biology of the Cell · doi:10.1091/mbc.12.10.2987 · Cited by 542
Dasika, G. K., Lin, S.-C. J., Zhao, S., Sung, P., Tomkinson, A., & Lee, E. Y.-H. P. (1999) · Oncogene · doi:10.1038/sj.onc.1203283 · Cited by 427
Recombination factors of Saccharomyces cerevisiae
Sung, P., Trujillo, K. M., & Van Komen, S. (2000) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/s0027-5107(00)00054-3 · Cited by 180
Metadata
- Authors
- Jiang, H., Xie, Y., Houston, P., Stemke-Hale, K., Mortensen, U. H., Rothstein, R., & Kodadek, T.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-12
- Volume
- 271
- Issue
- 52
- Pages
- 33181-33186
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2040015408
- Cited by
- 158
- References
- 0
- FWCI
- 4.95
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Jiang, H., Xie, Y., Houston, P., Stemke-Hale, K., Mortensen, U. H., Rothstein, R., & Kodadek, T. (1996). Direct Association between the Yeast Rad51 and Rad54 Recombination Proteins. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.52.33181
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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