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Overexpression of APOBEC-1 Results in Mooring Sequence-dependent Promiscuous RNA Editing

Sowden, Mark, Hamm, Jonathan K., Smith, Harold C.·1996·Journal of Biological Chemistry
DOI 10.1074/jbc.271.6.3011
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FIG. 1 Overexpression of APOBEC-1 by apobec-1 cDNA transfection. Approximately 40 m g of total cellular protein from null vector transfected, control McArdle ( 1 ) and 'high' APOBEC-1-expressing ( 2 ) cell lines were Western blotted and reacted with an anti-APOBEC-1 antibody (see 'Experimental Procedures'), and APOBEC-1 specific proteins were visualized using 125 I-labeled affinity-purified Protein A. Mr , migration of molecular weight standard proteins; arrowhead indicates APOBEC-1-specific immunoreactivity.
FIG. 1 Overexpression of APOBEC-1 by apobec-1 cDNA transfection. Approximately 40 m g of total cellular protein from null vector transfected, control McArdle ( 1 ) and 'high' APOBEC-1-expressing ( 2 ) cell lines were Western blotted and reacted with an anti-APOBEC-1 antibody (see 'Experimental Procedures'), and APOBEC-1 specific proteins were visualized using 125 I-labeled affinity-purified Protein A. Mr , migration of molecular weight standard proteins; arrowhead indicates APOBEC-1-specific immunoreactivity.CC-BY · From the PDF
FIG. 2 Additional editing site utilization in cells overexpressing APOBEC-1. Poisoned primer extension analyses were performed upon RT-PCR-amplified apoB DNA templates as described under 'Experimental Procedures.' Exogenous refers to the transfected human apoB RNA substrate; endogenous refers to the rat apoB RNA substrate. The positions of the primer and the extension products generated from unedited (CAA) and edited (UAA) RNAs are indicated. The length of these products is dependent upon the sequence 5 9 of the first edited C in the RNA substrate and is therefore different between the rat and human substrates. Longer primer extension products indicative of additional editing site utilization are indicated at 2 , 3 , and 4 .
FIG. 2 Additional editing site utilization in cells overexpressing APOBEC-1. Poisoned primer extension analyses were performed upon RT-PCR-amplified apoB DNA templates as described under 'Experimental Procedures.' Exogenous refers to the transfected human apoB RNA substrate; endogenous refers to the rat apoB RNA substrate. The positions of the primer and the extension products generated from unedited (CAA) and edited (UAA) RNAs are indicated. The length of these products is dependent upon the sequence 5 9 of the first edited C in the RNA substrate and is therefore different between the rat and human substrates. Longer primer extension products indicative of additional editing site utilization are indicated at 2 , 3 , and 4 .CC-BY · From the PDF
FIG. 3 Confirmation of additional editing site utilization on human apoB RNA by RT-PCR product sequencing. A , APOBEC-1overexpressing McArdle cell line; B , null vector-transfected, control McArdle cell line. The human apoB sequence is given (as C and T only), and site-specific (C 6666 ) and additional editing sites are indicated with an arrowhead . Only the C and T lanes are shown for clarity. Nucleotide coordinates are from the published human sequence (39).
FIG. 3 Confirmation of additional editing site utilization on human apoB RNA by RT-PCR product sequencing. A , APOBEC-1overexpressing McArdle cell line; B , null vector-transfected, control McArdle cell line. The human apoB sequence is given (as C and T only), and site-specific (C 6666 ) and additional editing sites are indicated with an arrowhead . Only the C and T lanes are shown for clarity. Nucleotide coordinates are from the published human sequence (39).CC-BY · From the PDF
FIG. 4 Confirmation of additional editing site utilization on rat apoB RNAbyRT-PCR product sequencing. Lanes 1-3 , APOBEC-1-overexpressing McArdle cell line; lanes 4 and 5 , control McArdle cell line. The rat apoB sequence is given (as C and T only), and site-specific (C 6666 ) and additional editing sites are indicated with an arrowhead . Only the C and T lanes are shown for clarity. Nucleotide coordinates are from the published rat sequence using the wild type editing site (C 6666 ) as an alignment point (40).
FIG. 4 Confirmation of additional editing site utilization on rat apoB RNAbyRT-PCR product sequencing. Lanes 1-3 , APOBEC-1-overexpressing McArdle cell line; lanes 4 and 5 , control McArdle cell line. The rat apoB sequence is given (as C and T only), and site-specific (C 6666 ) and additional editing sites are indicated with an arrowhead . Only the C and T lanes are shown for clarity. Nucleotide coordinates are from the published rat sequence using the wild type editing site (C 6666 ) as an alignment point (40).CC-BY · From the PDF
FIG. 5 Overexpression of APOBEC-1 overcomes the constraints of the spacer and regulator elements. The control wild type human apoB (wt) and the mutant substrate with imperfect spacer and regulator elements (TL) were independently, transiently transfected into the high APOBEC-1-expressing cell line. Poisoned primer extension analyses were performed as described in Fig. 2. The positions of the primer and the extension products generated from unedited (CAA) and edited (UAA) RNAs are indicated. Longer primer extension products indicative of additional editing site utilization are indicated ( 2 , 3 , and 4 ), their length being dependent upon RNA transcript sequence.
FIG. 5 Overexpression of APOBEC-1 overcomes the constraints of the spacer and regulator elements. The control wild type human apoB (wt) and the mutant substrate with imperfect spacer and regulator elements (TL) were independently, transiently transfected into the high APOBEC-1-expressing cell line. Poisoned primer extension analyses were performed as described in Fig. 2. The positions of the primer and the extension products generated from unedited (CAA) and edited (UAA) RNAs are indicated. Longer primer extension products indicative of additional editing site utilization are indicated ( 2 , 3 , and 4 ), their length being dependent upon RNA transcript sequence.CC-BY · From the PDF

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Research works printed but not linked: 40

  1. Chen, 1987, Science, 238, 363, 10.1126/science.3659919 · DOI
  2. Powell, 1987, Cell, 50, 831, 10.1016/0092-8674(87)90510-1 · DOI
  3. Lau, 1991, J. Biol. Chem., 266, 20550, 10.1016/S0021-9258(18)54960-7 · DOI
  4. Scott, 1994, Current Opin. Lipidol., 5, 87, 10.1097/00041433-199404000-00004 · DOI
  5. Shah, 1991, J. Biol. Chem., 266, 16301, 10.1016/S0021-9258(18)55296-0 · DOI
  6. Backus, 1991, Nucleic Acids Res., 19, 6781, 10.1093/nar/19.24.6781 · DOI
  7. Smith, 1991, Proc. Natl. Acad. Sci. U. S. A., 88, 1489, 10.1073/pnas.88.4.1489 · DOI
  8. Bostrom, 1989, J. Biol. Chem., 264, 15701, 10.1016/S0021-9258(19)84889-5 · DOI
  9. Driscoll, 1993, Mol. Cell. Biol., 13, 7288
  10. Backus, 1994, Biochim. Biophys. Acta, 1219, 1, 10.1016/0167-4781(94)90240-2 · DOI
  11. Harris, 1993, J. Biol. Chem., 268, 7382, 10.1016/S0021-9258(18)53186-0 · DOI
  12. Backus, 1992, Nucleic Acids Res., 20, 6007, 10.1093/nar/20.22.6007 · DOI
  13. Chen, 1990, J. Biol. Chem., 265, 6811, 10.1016/S0021-9258(19)39221-X · DOI
  14. Yao, 1992, J. Biol. Chem., 267, 1175, 10.1016/S0021-9258(18)48411-6 · DOI
  15. Navaratnam, 1993, J. Biol. Chem., 268, 20709, 10.1016/S0021-9258(19)36836-X · DOI
  16. Yamanaka, 1994, J. Biol. Chem., 269, 21725, 10.1016/S0021-9258(17)31865-3 · DOI
  17. Barnes, 1993, Biochem. Biophys. Res. Commun., 197, 1410, 10.1006/bbrc.1993.2634 · DOI
  18. Teng, 1993, Science, 260, 1816, 10.1126/science.8511591 · DOI · Paper page
  19. Bhattacharya, 1994, Trends Biochem. Sci., 19, 105, 10.1016/0968-0004(94)90200-3 · DOI
  20. Driscoll, 1994, J. Biol. Chem., 269, 19843, 10.1016/S0021-9258(17)32097-5 · DOI
  21. MacGinnitie, 1995, J. Biol. Chem., 270, 14768, 10.1074/jbc.270.24.14768 · DOI
  22. Giannoni, 1994, J. Biol. Chem., 269, 5932, 10.1016/S0021-9258(17)37551-8 · DOI
  23. Teng, 1994, J. Biol. Chem., 269, 29395, 10.1016/S0021-9258(18)43892-6 · DOI
  24. Chan, 1992, J. Biol. Chem., 267, 25621, 10.1016/S0021-9258(18)35646-1 · DOI
  25. Greeve, 1993, J. Lipid Res., 34, 1367, 10.1016/S0022-2275(20)36966-2 · DOI
  26. Navaratnam, 1995, Cell, 81, 187, 10.1016/0092-8674(95)90328-3 · DOI
  27. Anant, 1995, J. Biol. Chem., 270, 14762, 10.1074/jbc.270.24.14762 · DOI
  28. Navaratnam, 1993, Proc. Natl. Acad. Sci. U. S. A., 90, 222, 10.1073/pnas.90.1.222 · DOI
  29. Bostrom, 1990, J. Biol. Chem., 265, 22446, 10.1016/S0021-9258(18)45725-0 · DOI
  30. Chen, 1987, Mol. Cell. Biol., 7, 2745
  31. Driscoll, 1989, Cell, 58, 519, 10.1016/0092-8674(89)90432-7 · DOI · Paper page
  32. Davies, 1989, J. Biol. Chem., 264, 13395, 10.1016/S0021-9258(18)80008-4 · DOI
  33. Funahashi, 1995, J. Lipid Res., 36, 414, 10.1016/S0022-2275(20)39876-X · DOI
  34. Hadjiagapiou, 1994, Nucleic Acids Res., 22, 1874, 10.1093/nar/22.10.1874 · DOI
  35. Lau, 1994, Proc. Natl. Acad. Sci. U. S. A., 91, 8522, 10.1073/pnas.91.18.8522 · DOI
  36. Zannis, 1993, Adv. Hum. Genet., 21, 145
  37. Backus, 1994, Biochim. Biophys. Acta, 1217, 65, 10.1016/0167-4781(94)90126-0 · DOI
  38. Knott, 1986, Nucleic Acids Res., 14, 7501, 10.1093/nar/14.18.7501 · DOI
  39. Teng, 1990, Biochem. Biophys. Res. Commun., 173, 74, 10.1016/S0006-291X(05)81023-X · DOI
  40. Yamanaka, 1995, Proc. Natl. Acad. Sci. U. S. A., 92, 8483, 10.1073/pnas.92.18.8483 · DOI

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Metadata

Authors
Sowden, M., Hamm, J. K., & Smith, H. C.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1996-02
Volume
271
Issue
6
Pages
3011-3017
ISSN
0021-9258
DOI
10.1074/jbc.271.6.3011
Cited by
68
References
0
Language
en
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