The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Bcl-2 Phosphorylation Required for Anti-apoptosis Function
Open accessAbstract
The protooncogene Bcl-2 functions as a suppressor of apoptosis in growth factor-dependent cells, but a post-receptor signaling mechanism is not known. We recently reported that interleukin 3 (IL-3) and erythropoietin, or the protein kinase C activator bryostatin-1 (Bryo), not only suppresses apoptosis but also stimulates the phosphorylation of Bcl-2 (May, W. S., Tyler, P. G., Ito, T., Armstrong, D. K., Qatsha, K. A., and Davidson, N. E. (1994) J. Biol. Chem. 269, 26865-26870). To test whether phosphorylation is required for Bcl-2 function, conservative serine --> alanine mutations were produced at the seven putative protein kinase C phosphorylation sites in Bcl-2. Results indicate that the S70A Bcl-2 mutant fails to be phosphorylated after IL-3 or Bryo stimulation and is unable to support prolonged cell survival either upon IL-3 deprivation or etoposide treatment when compared with wild-type Bcl-2. In contrast, a Ser --> Glu mutant, S70E, which may mimic a potential phosphate charge, more potently suppressed the etoposide-induced apoptosis than wild type in the absence of IL-3. Since the loss of function S70A mutant can heterodimerize with its partner protein and death effector Bax, these findings demonstrate that Bcl-2:Bax heterodimerization is not sufficient and Bcl-2 phosphorylation is required for full Bcl-2 death suppressor signaling activity.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death
Oltval, Z. N., Milliman, C. L., & Korsmeyer, S. J. (1993) · Cell · doi:10.1016/0092-8674(93)90509-o · Cited by 6,003
Cited by
Signal transduction by the JNK group of MAP kinases
Davis, R. J. (2000) · Cell · doi:10.1016/s0092-8674(00)00116-1 · Cited by 4,237
Mitochondria and neuronal survival
Nicholls, D. G., & Budd, S. L. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.1.315 · Cited by 1,243
JNK promotes Bax translocation to mitochondria through phosphorylation of 14‐3‐3 proteins
Tsuruta, F., Sunayama, J., Mori, Y., Hattori, S., Shimizu, S., Tsujimoto, Y., Yoshioka, K., Masuyama, N., & Gotoh, Y. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600194 · Cited by 556
Domina, A. M., Vrana, J. A., Gregory, M. A., Hann, S. R., & Craig, R. W. (2004) · Oncogene · doi:10.1038/sj.onc.1207692 · Cited by 327
Vriend, J., & Reiter, R. J. (2015) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2014.12.013 · Cited by 246
Phosphorylation and Inactivation of Myeloid Cell Leukemia 1 by JNK in Response to Oxidative Stress
Inoshita, S., Takeda, K., Hatai, T., Terada, Y., Sano, M., Hata, J., Umezawa, A., & Ichijo, H. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m207951200 · Cited by 233
Robinson, B. H. (1998) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(98)00033-4 · Cited by 223
Regulation of Proliferation, Differentiation and Survival by the IL-3/IL-5/GM-CSF Receptor Family
de Groot, R. P., Coffer, P. J., & Koenderman, L. (1998) · Cellular Signalling · doi:10.1016/s0898-6568(98)00023-0 · Cited by 215
S-Nitrosylation of Bcl-2 Inhibits Its Ubiquitin-Proteasomal Degradation
Azad, N., Vallyathan, V., Wang, L., Tantishaiyakul, V., Stehlik, C., Leonard, S. S., & Rojanasakul, Y. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m602551200 · Cited by 194
Aquila, S., Sisci, D., Gentile, M., Middea, E., Catalano, S., Carpino, A., Rago, V., & Andò, S. (2004) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-031681 · Cited by 184
SCHROETER, H., BOYD, C. S., AHMED, R., SPENCER, J. P. E., DUNCAN, R. F., RICE-EVANS, C., & CADENAS, E. (2003) · Biochemical Journal · doi:10.1042/bj20030201 · Cited by 169
Mebendazole Induces Apoptosis via Bcl-2 Inactivation in Chemoresistant Melanoma Cells
Doudican, N., Rodriguez, A., Osman, I., & Orlow, S. J. (2008) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-07-2159 · Cited by 152
Changes in apoptosis and Bcl-2 expression in human hyperglycemic, term placental trophoblast
Sgarbosa, F., Barbisan, L. F., Brasil, M. A. M., Costa, E., Calderon, I. M. P., Gonçalves, C. R., Bevilacqua, E., & Rudge, M. V. C. (2006) · Diabetes Research and Clinical Practice · doi:10.1016/j.diabres.2005.12.014 · Cited by 80
Inflammation and survival pathways: Chronic lymphocytic leukemia as a model system
Chen, L. S., Balakrishnan, K., & Gandhi, V. (2010) · Biochemical Pharmacology · doi:10.1016/j.bcp.2010.07.039 · Cited by 55
Turzanski, J., Grundy, M., Russell, N. H., & Pallis, M. (2004) · Leukemia · doi:10.1038/sj.leu.2403457 · Cited by 51
Metadata
- Authors
- Ito, T., Deng, X., Carr, B., & May, W. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1997-05
- Volume
- 272
- Issue
- 18
- Pages
- 11671-11673
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1970834072
- Cited by
- 541
- References
- 1
- FWCI
- 14.40
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
