Absence of Glucocorticoid Receptor-β in Mice
Open accessAbstract
Two human glucocorticoid receptor (GR) isoforms, GRalpha and GRbeta, are derived from the same gene by alternative splicing involving exon 9 of the GR locus. The non-ligand binding isoform GRbeta was proposed to act as a transdominant negative inhibitor of GRalpha, thus modulating glucocorticoid responsiveness of target tissues. To study GRbeta in mice we characterized the genomic region around exon 9 of the murine GR gene. Sequence analysis revealed that the presumed exon 9beta contained an open reading frame of 59 amino acids. In contrast, human exon 9beta encoded only 15 amino acids. Using reverse transcriptase polymerase chain reaction the absence of GRbeta mRNA was demonstrated in all adult mouse tissues examined. To exclude the possibility that the polymerase chain reaction conditions employed were not suitable for the amplification of GRbeta mRNA, we synthesized an artificial template corresponding to the presumed GRbeta mRNA spanning exons 7, 8, and 9beta. Various amounts of this template were added to brain cDNA preparations and as little as 25 molecules were detectable under the polymerase chain reaction conditions chosen. Since GRbeta is not conserved across species its physiological significance in humans appears questionable.
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An equivalent of hGR-β has yet to be described in other species, including mice and rats.
“an equivalent of hGR-β has yet to be described in other species, including mice and rats.77,78”
From Cytokines and Glucocorticoid Receptor Signaling (2009) · cites this paper as [77]
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- Authors
- Otto, C., Reichardt, H. M., & Schütz, G.
- Affiliations
- German Cancer Research Center (DE)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1997-10
- Volume
- 272
- Issue
- 42
- Pages
- 26665-26668
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1484857303
- Cited by
- 102
- References
- 2
- Citations by year
- 2026: 1 · 2025: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2017: 6 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 4
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- glucocorticoid receptor beta, glucocorticoid receptor alpha, alternative splicing, exon 9, glucocorticoid responsiveness
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Exons, Humans, Molecular Sequence Data, Receptors, Glucocorticoid, Polymerase Chain Reaction, Alternative Splicing, DNA, Complementary, Mice
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