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Isolation of a Δ5-Fatty Acid Desaturase Gene fromMortierella alpina
Open accessAbstract
Arachidonic acid (C20:4 Delta5,8,11,14) is a polyunsaturated fatty acid synthesized by the Delta5-fatty acid desaturation of di-homo-gamma-linolenic acid (C20:3 Delta8,11,14). In mammals, it is known to be a precursor of the prostaglandins and the leukotrienes but it is also accumulated by the filamentous fungus Mortierella alpina. We have isolated a cDNA encoding the Delta5-fatty acid desaturase from M. alpina via a polymerase chain reaction-based strategy using primers designed to the conserved histidine box regions of microsomal desaturases, and confirmed its function by expression in the yeast Saccharomyces cerevisiae. Analysis of the lipids from the transformed yeast demonstrated the accumulation of arachidonic acid. The M. alpina Delta5-desaturase is the first example of a cloned Delta5-desaturase, and differs from other fungal desaturases previously characterized by the presence of an N-terminal domain related to cytochrome b5.
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Cited by
Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants
Qi, B., Fraser, T., Mugford, S., Dobson, G., Sayanova, O., Butler, J., Napier, J. A., Stobart, A. K., & Lazarus, C. M. (2004) · Nature Biotechnology · doi:10.1038/nbt972 · Cited by 426
Self-citationBergé, J.-P., & Barnathan, G. (2005) · Advances in Biochemical Engineering/Biotechnology · doi:10.1007/b135782 · Cited by 401
Abbadi, A., Domergue, F., Bauer, J., Napier, J. A., Welti, R., Zähringer, U., Cirpus, P., & Heinz, E. (2004) · The Plant Cell · doi:10.1105/tpc.104.026070 · Cited by 322
Self-citationBiological Role of Unsaturated Fatty Acid Desaturases in Health and Disease
Czumaj, A., & Śledziński, T. (2020) · Nutrients · doi:10.3390/nu12020356 · Cited by 150
Biosynthesis of docosahexaenoic acid (DHA, 22:6-4, 7,10,13,16,19): Two distinct pathways
Qiu, X. (2003) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/s0952-3278(02)00268-5 · Cited by 149
Metadata
- Authors
- Michaelson, L. V., Lazarus, C. M., Griffiths, G., Napier, J. A., & Stobart, A. K.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-07
- Volume
- 273
- Issue
- 30
- Pages
- 19055-19059
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2050630630
- Cited by
- 161
- References
- 0
- FWCI
- 6.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Michaelson, L. V., Lazarus, C. M., Griffiths, G., Napier, J. A., & Stobart, A. K. (1998). Isolation of a Δ5-Fatty Acid Desaturase Gene fromMortierella alpina. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.273.30.19055
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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