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A Sphingolipid Desaturase from Higher Plants
Open accessAbstract
A recently cloned cDNA from sunflower codes for a fusion protein composed of an N-terminal cytochrome b5 and a domain similar to membrane-bound acyl lipid desaturases. For a functional identification, homologous cDNAs from Brassica napus and Arabidopsis thaliana were expressed in Saccharomyces cerevisiae, and sphingolipid long chain bases were analyzed. The expression of the heterologous enzyme results in significant proportions of new Delta8, 9-cis/trans-phytosphingenines that accompany the residual C18-phytosphinganine predominating in wild-type yeast cells. These results represent the first identification of a gene coding for a sphingolipid desaturase and for a stereounselective desaturase showing trans-activity from any organism. Furthermore, this fusion protein is a new member of the cytochrome b5 superfamily. The formation of the two regioisomeric phytosphingenines in the transformed yeast sheds new light on the factors controlling regioselectivity.
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Cited by
Zheng, W., Kollmeyer, J., Symolon, H., Momin, A., Munter, E., Wang, E., Kelly, S., Allegood, J. C., Liu, Y., Peng, Q., et al. (2006) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2006.08.009 · Cited by 560
Li-Beisson, Y., Shorrosh, B., Beisson, F., Andersson, M. X., Arondel, V., Bates, P. D., Baud, S., Bird, D., DeBono, A., Durrett, T. P., et al. (2010) · The Arabidopsis Book · doi:10.1199/tab.0133 · Cited by 367
Identification and Characterization of a Sphingolipid Δ4-Desaturase Family
Ternes, P., Franke, S., Zähringer, U., Sperling, P., & Heinz, E. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202947200 · Cited by 293
Self-citationPlant sphingolipids: structural diversity, biosynthesis, first genes and functions
Sperling, P., & Heinz, E. (2003) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(03)00033-7 · Cited by 286
Self-citationBiosynthesis of docosahexaenoic acid (DHA, 22:6-4, 7,10,13,16,19): Two distinct pathways
Qiu, X. (2003) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/s0952-3278(02)00268-5 · Cited by 149
Metadata
- Authors
- Sperling, P., Zähringer, U., & Heinz, E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-11
- Volume
- 273
- Issue
- 44
- Pages
- 28590-28596
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1969211270
- Cited by
- 166
- References
- 0
- FWCI
- 3.04
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Sperling, P., Zähringer, U., & Heinz, E. (1998). A Sphingolipid Desaturase from Higher Plants. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.273.44.28590
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
