The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
MAPKAP Kinase 2 Phosphorylates Serum Response Factor in Vitro and in Vivo
Open accessAbstract
Several growth factor- and calcium-regulated kinases such as pp90(rsk) or CaM kinase IV can phosphorylate the transcription factor serum response factor (SRF) at serine 103 (Ser-103). However, it is unknown whether stress-regulated kinases can also phosphorylate SRF. We show that treatment of cells with anisomycin, arsenite, sodium fluoride, or tetrafluoroaluminate induces phosphorylation of SRF at Ser-103 in both HeLa and NIH3T3 cells. This phosphorylation is dependent on the kinase p38/SAPK2 and correlates with the activation of MAPKAP kinase 2 (MK2). MK2 phosphorylates SRF in vitro at Ser-103 with similar efficiency as the small heat shock protein Hsp25 and significantly better than CREB. Comparison of wild type murine fibroblasts with those derived from MK2-deficient mice (Mk(-/-)) reveals MK2 as the major SRF kinase induced by arsenite. These results demonstrate that SRF is targeted by several signal transduction pathways within cells and establishes SRF as a nuclear target for MAPKAP kinase 2.
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Cites
Independent Human MAP-Kinase Signal Transduction Pathways Defined by MEK and MKK Isoforms
Dérijard, B., Raingeaud, J., Barrett, T., Wu, I.-H., Han, J., Ulevitch, R. J., & Davis, R. J. (1995) · Science · doi:10.1126/science.7839144 · Cited by 1,555
Cited by
Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases
Cargnello, M., & Roux, P. P. (2011) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00031-10 · Cited by 3,274
Chen, Z., Gibson, T. B., Robinson, F., Silvestro, L., Pearson, G., Xu, B.-E., Wright, A., Vanderbilt, C., & Cobb, M. H. (2001) · Chemical Reviews · doi:10.1021/cr000241p · Cited by 788
Greer, P. L., & Greenberg, M. E. (2008) · Neuron · doi:10.1016/j.neuron.2008.09.002 · Cited by 661
Transcriptional regulation by the MAP kinase signaling cascades
Yang, S.-H., Sharrocks, A. D., & Whitmarsh, A. J. (2003) · Gene · doi:10.1016/s0378-1119(03)00816-3 · Cited by 494
Mammalian heat shock response and mechanisms underlying its genome-wide transcriptional regulation
Mahat, D. B., Salamanca, H. H., Duarte, F. M., Danko, C. G., & Lis, J. T. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.02.025 · Cited by 426
Metadata
- Authors
- Heidenreich, O., Neininger, A., Schratt, G., Zinck, R., Cahill, M. A., Engel, K., Kotlyarov, A., Kraft, R., Kostka, S., Gaestel, M., & Nordheim, A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-05
- Volume
- 274
- Issue
- 20
- Pages
- 14434-14443
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2161314639
- Cited by
- 170
- References
- 1
- FWCI
- 5.55
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: MAPKAP Kinase 2 Phosphorylates Serum Response Factor in Vitro and in Vivo. https://app.cochat.ai/papers/10.1074/jbc.274.20.14434
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- Heidenreich, O., Neininger, A., Schratt, G., Zinck, R., Cahill, M. A., Engel, K., Kotlyarov, A., Kraft, R., Kostka, S., Gaestel, M., & Nordheim, A. (1999). MAPKAP Kinase 2 Phosphorylates Serum Response Factor in Vitro and in Vivo. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.20.14434
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