The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Identification of a Membrane Protein, LAT-2, That Co-expresses with 4F2 Heavy Chain, an L-type Amino Acid Transport Activity with Broad Specificity for Small and Large Zwitterionic Amino Acids
Open accessAbstract
We have identified a new human cDNA, L-amino acid transporter-2 (LAT-2), that induces a system L transport activity with 4F2hc (the heavy chain of the surface antigen 4F2, also named CD98) in oocytes. Human LAT-2 is the fourth member of the family of amino acid transporters that are subunits of 4F2hc. The amino acid transport activity induced by the co-expression of 4F2hc and LAT-2 was sodium-independent and showed broad specificity for small and large zwitterionic amino acids, as well as bulky analogs (e.g. BCH (2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid)). This transport activity was highly trans-stimulated, suggesting an exchanger mechanism of transport. Expression of tagged N-myc-LAT-2 alone in oocytes did not induce amino acid transport, and the protein had an intracellular location. Co-expression of N-myc-LAT-2 and 4F2hc gave amino acid transport induction and expression of N-myc-LAT-2 at the plasma membrane of the oocytes. These data suggest that LAT-2 is an additional member of the family of 4F2 light chain subunits, which associates with 4F2hc to express a system L transport activity with broad specificity for zwitterionic amino acids. Human LAT-2 mRNA is expressed in kidney >>> placenta >> brain, liver > spleen, skeletal muscle, heart, small intestine, and lung. Human LAT-2 gene localizes at chromosome 14q11.2-13 (13 cR or approximately 286 kb from marker D14S1349). The high expression of LAT-2 mRNA in epithelial cells of proximal tubules, the basolateral location of 4F2hc in these cells, and the amino acid transport activity of LAT-2 suggest that this transporter contributes to the renal reabsorption of neutral amino acids in the basolateral domain of epithelial proximal tubule cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Structural features in eukaryotic mRNAs that modulate the initiation of translation.
Kozak, M. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54860-2 · Cited by 1,836
Kanai, Y., Segawa, H., Miyamoto, K.-I., Uchino, H., Takeda, E., & Endou, H. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.37.23629 · Cited by 1,109
Role of amino acid transport and countertransport in nutrition and metabolism.
Christensen, H. N. (1990) · Physiological Reviews · doi:10.1152/physrev.1990.70.1.43 · Cited by 1,099
Molecular Biology of Mammalian Plasma Membrane Amino Acid Transporters
PALACÍN, M., ESTÉVEZ, R., BERTRAN, J., & ZORZANO, A. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.4.969 · Cited by 823
Self-citationFelig, P. (1975) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.44.070175.004441 · Cited by 789
Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family
Mastroberardino, L., Spindler, B., Pfeiffer, R., Skelly, P. J., Loffing, J., Shoemaker, C. B., & Verrey, F. (1998) · Nature · doi:10.1038/26246 · Cited by 557
Transporters for Cationic Amino Acids in Animal Cells: Discovery, Structure, and Function
DEVÉS, R., & BOYD, C. A. R. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.2.487 · Cited by 499
Cystinuria caused by mutations in rBAT, a gene involved in the transport of cystine
Calonge, M. J., Gasparini, P., Chillarón, J., Chillón, M., Gallucci, M., Rousaud, F., Zelante, L., Testar, X., Dallapiccola, B., Di Silverio, F., et al. (1994) · Nature Genetics · doi:10.1038/ng0494-420 · Cited by 379
Self-citationTorrents, D., Estévez, R., Pineda, M., Fernández, E., Lloberas, J., Shi, Y.-B., Zorzano, A., & Palacı́n, M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.49.32437 · Cited by 337
Self-citationSibson, N. R., Dhankhar, A., Mason, G. F., Behar, K. L., Rothman, D. L., & Shulman, R. G. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.6.2699 · Cited by 320
Cited by
Multiple types of skeletal muscle atrophy involve a common program of changes in gene expression
Lecker, S. H., Jagoe, R. T., Gilbert, A., Gomes, M., Baracos, V., Bailey, J., Price, S. R., Mitch, W. E., & Goldberg, A. L. (2004) · The FASEB Journal · doi:10.1096/fj.03-0610com · Cited by 1,539
Koppula, P., Zhang, Y., Zhuang, L., & Gan, B. (2018) · Cancer Communications · doi:10.1186/s40880-018-0288-x · Cited by 915
Yanagida, O., Kanai, Y., Chairoungdua, A., Kim, D. K., Segawa, H., Nii, T., Cha, S. H., Matsuo, H., Fukushima, J.-I., Fukasawa, Y., et al. (2001) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(01)00384-4 · Cited by 746
CATs and HATs: the SLC7 family of amino acid transporters
Verrey, F., Closs, E. I., Wagner, C. A., Palacin, M., Endou, H., & Kanai, Y. (2004) · Pfl�gers Archiv European Journal of Physiology · doi:10.1007/s00424-003-1086-z · Cited by 710
Selective expression of the large neutral amino acid transporter at the blood–brain barrier
Boado, R. J., Li, J. Y., Nagaya, M., Zhang, C., & Pardridge, W. M. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.21.12079 · Cited by 481
Metadata
- Authors
- Pineda, M., Fernández, E., Torrents, D., Estévez, R., López, C., Camps, M., Lloberas, J., Zorzano, A., & Palacı́n, M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-07
- Volume
- 274
- Issue
- 28
- Pages
- 19738-19744
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1996045753
- Cited by
- 408
- References
- 31
- FWCI
- 13.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Identification of a Membrane Protein, LAT-2, That Co-expresses with 4F2 Heavy Chain, an L-type Amino Acid Transport Activity with Broad Specificity for Small and Large Zwitterionic Amino Acids. https://app.cochat.ai/papers/10.1074/jbc.274.28.19738
- The paper
- Pineda, M., Fernández, E., Torrents, D., Estévez, R., López, C., Camps, M., Lloberas, J., Zorzano, A., & Palacı́n, M. (1999). Identification of a Membrane Protein, LAT-2, That Co-expresses with 4F2 Heavy Chain, an L-type Amino Acid Transport Activity with Broad Specificity for Small and Large Zwitterionic Amino Acids. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.28.19738
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
