The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Binding of 14-3-3 Protein to the Plasma Membrane H+-ATPase AHA2 Involves the Three C-terminal Residues Tyr946-Thr-Val and Requires Phosphorylation of Thr947
Open accessAbstract
14-3-3 proteins play a regulatory role in a diverse array of cellular functions such as apoptosis, regulation of the cell cycle, and regulation of gene transcription. The phytotoxin fusicoccin specifically induces association of virtually any 14-3-3 protein to plant plasma membrane H(+)-ATPase. The 14-3-3 binding site in the Arabidopsis plasma membrane H(+)-ATPase AHA2 was localized to the three C-terminal residues of the enzyme (Tyr(946)-Thr-Val). Binding of 14-3-3 protein to this target was induced by phosphorylation of Thr(947) (K(D) = 88 nM) and was in practice irreversible in the presence of fusicoccin (K(D) = 7 nM). Mass spectrometry analysis demonstrated that AHA2 expressed in yeast was phosphorylated at Thr(947). We conclude that the extreme end of AHA2 contains an unusual high-affinity binding site for 14-3-3 protein.
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Cited by
Leyser, O. (2018) · Plant Physiology · doi:10.1104/pp.17.00765 · Cited by 715
Closing gaps: linking elements that control stomatal movement
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H + -ATPases in Plant Growth and Stress Responses
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The molecular mechanism of plasma membrane H+-ATPases in plant responses to abiotic stress
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Metadata
- Authors
- Fuglsang, A. T., Visconti, S., Drumm, K., Jahn, T., Stensballe, A., Mattei, B., Jensen, O. N., Aducci, P., & Palmgren, M. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-12
- Volume
- 274
- Issue
- 51
- Pages
- 36774-36780
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1989060957
- Cited by
- 342
- References
- 35
- FWCI
- 4.89
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Binding of 14-3-3 Protein to the Plasma Membrane H+-ATPase AHA2 Involves the Three C-terminal Residues Tyr946-Thr-Val and Requires Phosphorylation of Thr947. https://app.cochat.ai/papers/10.1074/jbc.274.51.36774
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- Fuglsang, A. T., Visconti, S., Drumm, K., Jahn, T., Stensballe, A., Mattei, B., Jensen, O. N., Aducci, P., & Palmgren, M. G. (1999). Binding of 14-3-3 Protein to the Plasma Membrane H+-ATPase AHA2 Involves the Three C-terminal Residues Tyr946-Thr-Val and Requires Phosphorylation of Thr947. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.51.36774
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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