The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
The Wilms' Tumor 1 Tumor Suppressor Gene Represses Transcription of the Human Telomerase Reverse Transcriptase Gene
Open accessAbstract
Regulation of the human telomerase reverse transcriptase (hTERT) gene is the primary determinant for telomerase enzyme activity, which is found in tumor cells but is largely absent from normal somatic cells. Recent studies have shown that Myc protein can transcriptionally activate the hTERT gene. However, little is known about the repression mechanism of the hTERT gene and telomerase enzyme. Here, we developed an expression cloning strategy to identify cDNAs whose products can repress hTERT promoter activity in telomerase-positive immortal cells. Using this screen, we isolated the Wilms' tumor 1 suppressor gene (WT1). WT1 can repress hTERT promoter activity in 293 kidney cells. The WT1 binding site on the hTERT promoter was identified by deletional analysis. Alteration of the WT1 binding site markedly derepresses transcription from an isolated hTERT promoter by inhibiting interaction of WT1 with DNA. These specific repression effects of WT1 were not observed in HeLa cells, which express no endogenous WT1. Furthermore, we show that WT1 can repress the endogenous hTERT promoter and telomerase enzyme activities. These results suggest that WT1 may be a transcriptional repressor of the hTERT gene, at least in some specific cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Several transcriptional repressors, including Wilms' tumor 1 tumor suppressor (WT1) and myeloidspecific zinc finger protein-2 (MZF-2) are also known to repress hTERT transcription via binding to their specific sites on the promoter, although the mechanisms of repression remain unclear.(73,74)”
From Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers (2008) · cites this paper as [73]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Specific Association of Human Telomerase Activity with Immortal Cells and Cancer
Kim, N. W., Piatyszek, M. A., Prowse, K. R., Harley, C. B., West, M. D., Ho, P. L. C., Coviello, G. M., Wright, W. E., Weinrich, S. L., & Shay, J. W. (1994) · Science · doi:10.1126/science.7605428 · Cited by 7,091
Extension of Life-Span by Introduction of Telomerase into Normal Human Cells
Bodnar, A. G., Ouellette, M., Frolkis, M., Holt, S. E., Chiu, C.-P., Morin, G. B., Harley, C. B., Shay, J. W., Lichtsteiner, S., & Wright, W. E. (1998) · Science · doi:10.1126/science.279.5349.349 · Cited by 4,975
A survey of telomerase activity in human cancer
Shay, J. W., & Bacchetti, S. (1997) · European Journal of Cancer · doi:10.1016/s0959-8049(97)00062-2 · Cited by 2,794
Creation of human tumour cells with defined genetic elements
Hahn, W. C., Counter, C. M., Lundberg, A. S., Beijersbergen, R. L., Brooks, M. W., & Weinberg, R. A. (1999) · Nature · doi:10.1038/22780 · Cited by 2,345
Telomerase Catalytic Subunit Homologs from Fission Yeast and Human
Nakamura, T. M., Morin, G. B., Chapman, K. B., Weinrich, S. L., Andrews, W. H., Lingner, J., Harley, C. B., & Cech, T. R. (1997) · Science · doi:10.1126/science.277.5328.955 · Cited by 2,256
Meyerson, M., Counter, C. M., Eaton, E. N., Ellisen, L. W., Steiner, P., Caddle, S. D., Ziaugra, L., Beijersbergen, R. L., Davidoff, M. J., Liu, Q., et al. (1997) · Cell · doi:10.1016/s0092-8674(00)80538-3 · Cited by 1,784
Vaziri, H., & Benchimol, S. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70109-5 · Cited by 1,003
Direct activation of TERT transcription by c-MYC
Wu, K.-J., Grandori, C., Amacker, M., Simon-Vermot, N., Polack, A., Lingner, J., & Dalla-Favera, R. (1999) · Nature Genetics · doi:10.1038/6010 · Cited by 893
Kilian, A., Bowtell, D. D. L., Abud, H. E., Hime, G. R., Venter, D. J., Keese, P., Duncan, E. L., Reddel, R. R., & Jefferson, R. (1997) · Human Molecular Genetics · doi:10.1093/hmg/6.12.2011 · Cited by 592
Cong, Y. (1999) · Human Molecular Genetics · doi:10.1093/hmg/8.1.137 · Cited by 485
高倉, 正. (2002) · Medical Entomology and Zoology · Cited by 475
The telomerase reverse transcriptase: components and regulationPreprint
Nugent, C. I., & Lundblad, V. (1998) · Genes & Development · doi:10.1101/gad.12.8.1073 · Cited by 435
Greenberg, R. A., O’Hagan, R. C., Deng, H., Xiao, Q., Hann, S. R., Adams, R. R., Lichtsteiner, S., Chin, L., Morin, G. B., & DePinho, R. A. (1999) · Oncogene · doi:10.1038/sj.onc.1202669 · Cited by 411
Telomerase activity, cell proliferation, and cancer
Greider, C. W. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.1.90 · Cited by 408
Regulation of Interferon-γ-Activated STAT1 by the Ubiquitin-Proteasome Pathway
Kim, T. K., & Maniatis, T. (1996) · Science · doi:10.1126/science.273.5282.1717 · Cited by 405
Self-citationCloning and characterization of the promoter region of human telomerase reverse transcriptase gene.
Horikawa, I., Cable, P. L., Afshari, C. A., & Barrett, J. C. (1999) · PubMed · Cited by 393
The Mechanism of Transcriptional Synergy of an In Vitro Assembled Interferon-β Enhanceosome
Kim, T. K., & Maniatis, T. (1997) · Molecular Cell · doi:10.1016/s1097-2765(00)80013-1 · Cited by 340
Self-citationSedivy, J. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.16.9078 · Cited by 182
Ning, Y., Weber, J. L., Killary, A. M., Ledbetter, D. H., Smith, J. R., & Pereira‐Smith, O. M. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.13.5635 · Cited by 176
The role of WT1 in Wilms tumorigenesis
Coppes, M. J., Campbell, C. E., & Williams, B. (1993) · The FASEB Journal · doi:10.1096/fasebj.7.10.8393819 · Cited by 153
Inactivation of WT1 in nephrogenic rests, genetic precursors to Wilms' tumour
Park, S., Bernard, A., Bove, K. E., Sens, D. A., Hazen‐Martin, D. J., Garvin, A. J., & Haber, D. A. (1993) · Nature Genetics · doi:10.1038/ng1293-363 · Cited by 143
Restoration of the Cellular Senescence Program and Repression of Telomerase by Human Chromosome 3
Ohmura, H., Tahara, H., Suzuki, M., Ide, T., Shimizu, M., Yoshida, M. A., Tahara, E., Shay, J. W., Barrett, J. C., & Oshimura, M. (1995) · Japanese Journal of Cancer Research · doi:10.1111/j.1349-7006.1995.tb02998.x · Cited by 124
Time, telomeres and tumours: is cellular senescence more than an anticancer mechanism?
Wright, W. E., & Shay, J. W. (1995) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)89044-3 · Cited by 119
Cuthbert, A. P., Bond, J., Trott, D. A., Gill, S., Broni, J., Marriott, A., Khoudoli, G., Parkinson, E. K., Cooper, C. S., & Newbold, R. F. (1999) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/91.1.37 · Cited by 114
Effects of activation-defective TBP mutations on transcription initiation in yeast
Kim, T. K., Hashimoto, S., Kelleher, R. J. I., Flanagan, P., Kornberg, R., Horikoshi, M., & Roeder, R. (1994) · Nature · doi:10.1038/369252a0 · Cited by 114
Self-citationA Gene Involved in Control of Human Cellular Senescence on Human Chromosome 1q
Hensler, P. J., Annab, L. A., Barrett, J. C., & Pereira‐Smith, O. M. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.4.2291 · Cited by 95
Multiple pathways for the regulation of telomerase activity
Holt, S. E., Wright, W. E., & Shay, J. (1997) · European Journal of Cancer · doi:10.1016/s0959-8049(97)00066-x · Cited by 93
Hastie, N. D. (1992) · Human Molecular Genetics · doi:10.1093/hmg/1.5.293 · Cited by 79
Oh, S., Song, Y., Kim, U.-J., Yim, J., & Kim, T. K. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.1366 · Cited by 71
Self-citationGuan, L., Rauchman, M. I., & Wang, Z. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.42.27047 · Cited by 61
WT1—more than a transcription factor?
Englert, C. (1998) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(98)01277-8 · Cited by 56
Toward Identifying a Cellular Determinant of Telomerase Repression
Shay, J. W. (1999) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/91.1.4 · Cited by 39
Ogata, T., Oshimura, M., Namba, M., Fujii, M., Oishi, M., & Ayusawa, D. (1995) · Japanese Journal of Cancer Research · doi:10.1111/j.1349-7006.1995.tb02985.x · Cited by 32
Rauscher, F. J. (1997) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-60479-9_10 · Cited by 15
Research works printed but not linked: 5
- Takakura, 1999, Cancer Res., 59, 551
- Horikawa, 1999, Cancer Res., 59, 826
- Rauscher, 1997, Curr. Top. Microbiol. Immunol., 220, 151
- Haber, 1992, New Biol., 4, 97
- Hensler, 1994, Mol. Cell. Biol., 14, 2291
Cited by
Sorted by citations
Human Telomerase and Its Regulation
Cong, Y.-S., Wright, W. E., & Shay, J. W. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.3.407-425.2002 · Cited by 898
A tumor suppressor and oncogene: the WT1 story
Yang, L., Han, Y., Saiz, F. S., & Minden, M. D. (2007) · Leukemia · doi:10.1038/sj.leu.2404624 · Cited by 480
WT1 proteins: functions in growth and differentiation
Scharnhorst, V., van der Eb, A. J., & Jochemsen, A. G. (2001) · Gene · doi:10.1016/s0378-1119(01)00593-5 · Cited by 355
Natural and pharmacological regulation of telomerase
Mergny, J. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.4.839 · Cited by 355
Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers
Kyo, S., Takakura, M., Fujiwara, T., & Inoue, M. (2008) · Cancer Science · doi:10.1111/j.1349-7006.2008.00878.x · Cited by 347
Metadata
- Authors
- Oh, S., Song, Y., Yim, J., & Kim, T. K.
- Affiliations
- Seoul National University (KR)
- Harvard University (US)
- Corresponding author
- Kim, Tae Kook
- ORCID
- Oh, Sangtaek
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-12
- Volume
- 274
- Issue
- 52
- Pages
- 37473-37478
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2046268297
- Cited by
- 148
- References
- 35
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 7 · 2013: 6 · 2012: 3
- FWCI
- 6.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- Wilms tumor 1, WT1, telomerase reverse transcriptase, hTERT, transcriptional repression, telomerase, promoter regulation, transcriptional repressor, c-Myc, expression cloning, kidney cells
- MeSH terms
- DNA-Binding Proteins, HeLa Cells, Humans, Promoter Regions, Genetic, Repressor Proteins, RNA, Transcription Factors, Transcription, Genetic, Genes, Tumor Suppressor, Telomerase, WT1 Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
