The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
High Density Lipoprotein Prevents Oxidized Low Density Lipoprotein-induced Inhibition of Endothelial Nitric-oxide Synthase Localization and Activation in Caveolae
Open accessAbstract
Oxidized LDL (oxLDL) depletes caveolae of cholesterol, resulting in the displacement of endothelial nitric-oxide synthase (eNOS) from caveolae and impaired eNOS activation. In the present study, we determined if the class B scavenger receptors, CD36 and SR-BI, are involved in regulating nitric-oxide synthase localization and function. We demonstrate that CD36 and SR-BI are expressed in endothelial cells, co-fractionate with caveolae, and co-immunoprecipitate with caveolin-1. Co-incubation of cells with 10 microgram/ml high density lipoprotein (HDL) prevented oxLDL-induced translocation of eNOS from caveolae and restored acetylcholine-induced nitric-oxide synthase stimulation. Acetylcholine caused eNOS activation in cells incubated with 10 microgram/ml oxLDL (10-15 thiobarbituric acid-reactive substances) and blocking antibodies to CD36, whereas cells treated with only oxLDL were unresponsive. Furthermore, CD36-blocking antibodies prevented oxLDL-induced redistribution of eNOS. SR-BI-blocking antibodies were used to demonstrate that the effects of HDL are mediate by SR-BI. HDL binding to SR-BI maintained the concentration of caveola-associated cholesterol by promoting the uptake of cholesterol esters, thereby preventing oxLDL-induced depletion of caveola cholesterol. We conclude that CD36 mediates the effects of oxLDL on caveola composition and eNOS activation. Furthermore, HDL prevents oxLDL from decreasing the capacity for eNOS activation by preserving the cholesterol concentration in caveolae and, thereby maintaining the subcellular location of eNOS.
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1 retracted
Characterization of a Cytosolic Heat-shock Protein-Caveolin Chaperone Complex
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Acylation Targets Endothelial Nitric-oxide Synthase to Plasmalemmal Caveolae
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Self-citation
Cited by
Oxidized LDL: Diversity, Patterns of Recognition, and Pathophysiology
Levitan, I., Volkov, S., & Subbaiah, P. V. (2010) · Antioxidants & Redox Signaling · doi:10.1089/ars.2009.2733 · Cited by 473
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Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells
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Oxidized LDL and HDL: antagonists in atherothrombosis
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Caveolin, Caveolae, and Endothelial Cell Function
Frank, P. G., Woodman, S. E., Park, D. S., & Lisanti, M. P. (2003) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000070546.16946.3a · Cited by 384
Metadata
- Authors
- Uittenbogaard, A., Shaul, P. W., Yuhanna, I. S., Blair, A., & Smart, E. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-04
- Volume
- 275
- Issue
- 15
- Pages
- 11278-11283
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2051134788
- Cited by
- 310
- References
- 36
- FWCI
- 8.99
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Uittenbogaard, A., Shaul, P. W., Yuhanna, I. S., Blair, A., & Smart, E. J. (2000). High Density Lipoprotein Prevents Oxidized Low Density Lipoprotein-induced Inhibition of Endothelial Nitric-oxide Synthase Localization and Activation in Caveolae. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.275.15.11278
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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