The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Structural Analysis of Glycosaminoglycans inDrosophila and Caenorhabditis elegans and Demonstration That tout-velu, a Drosophila Gene Related to EXT Tumor Suppressors, Affects Heparan Sulfate in Vivo
Open accessAbstract
We have devised a sensitive method for the isolation and structural analysis of glycosaminoglycans from two genetically tractable model organisms, the fruit fly, Drosophila melanogaster, and the nematode, Caenorhabditis elegans. We detected chondroitin/chondroitin sulfate- and heparan sulfate-derived disaccharides in both organisms. Chondroitinase digestion of glycosaminoglycans from adult Drosophila produced both nonsulfated and 4-O-sulfated unsaturated disaccharides, whereas only unsulfated forms were detected in C. elegans. Heparin lyases released disaccharides bearing N-, 2-O-, and 6-O-sulfated species, including mono-, di-, and trisulfated forms. We observed tissue- and stage-specific differences in both chondroitin sulfate and heparan sulfate composition in Drosophila. We have also applied these methods toward the analysis of tout-velu, an EXT-related gene in Drosophila that controls the tissue distribution of the growth factor Hedgehog. The proteins encoded by the vertebrate tumor suppressor genes EXT1 and 2, show heparan sulfate co-polymerase activity, and it has been proposed that tout-velu affects Hedgehog activity via its role in heparan sulfate biosynthesis. Analysis of total glycosaminoglycans from tout-velu mutant larvae show marked reductions in heparan sulfate but not chondroitin sulfate, consistent with its proposed function as a heparan sulfate co-polymerase.
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Cites
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Cited by
Order Out of Chaos: Assembly of Ligand Binding Sites in Heparan Sulfate
Esko, J. D., & Selleck, S. B. (2002) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.71.110601.135458 · Cited by 1,495
Self-citationFunctions of heparan sulfate proteoglycans in cell signaling during development
Lin, X. (2004) · Development · doi:10.1242/dev.01522 · Cited by 678
Disruption of Gastrulation and Heparan Sulfate Biosynthesis in EXT1-Deficient Mice
Lin, X., Wei, G., Shi, Z., Dryer, L., Esko, J. D., Wells, D. E., & Matzuk, M. M. (2000) · Developmental Biology · doi:10.1006/dbio.2000.9798 · Cited by 427
Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans
Mizuguchi, S., Uyama, T., Kitagawa, H., Nomura, K. H., Dejima, K., Gengyo-Ando, K., Mitani, S., Sugahara, K., & Nomura, K. (2003) · Nature · doi:10.1038/nature01635 · Cited by 260
Heparan Sulfate Structure in Mice with Genetically Modified Heparan Sulfate Production
Ledin, J., Staatz, W., Li, J.-P., Götte, M., Selleck, S., Kjellén, L., & Spillmann, D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m405382200 · Cited by 230
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Metadata
- Authors
- Toyoda, H., Kinoshita-Toyoda, A., & Selleck, S. B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-01
- Volume
- 275
- Issue
- 4
- Pages
- 2269-2275
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2087336633
- Cited by
- 287
- References
- 34
- FWCI
- 9.36
- Type
- journal article
- Language
- en
- Open access
- hybrid
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