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Fatty Acid and Lipoic Acid Biosynthesis in Higher Plant Mitochondria
Open accessAbstract
Fatty acid and lipoic acid biosynthesis were investigated in plant mitochondria. Although the mitochondria lack acetyl-CoA carboxylase, our experiments reveal that they contain the enzymatic equipment necessary to transform malonate into the two main building units for fatty acid synthesis: malonyl- and acetyl-acyl carrier protein (ACP). We demonstrated, by a new method based on a complementary use of high performance liquid chromatography and mass spectrometry, that the soluble mitochondrial fatty-acid synthase produces mainly three predominant acyl-ACPs as follows: octanoyl(C8)-, hexadecanoyl(C16)-, and octadecanoyl(C18)-ACP. Octanoate production is of primary interest since it has been postulated long ago to be a precursor of lipoic acid. By using a recombinant H apoprotein mutant as a potential acceptor for newly synthesized lipoic acid, we were able to detect limited amounts of lipoylated H protein in the presence of malonate, several sulfur donors, and cofactors. Finally, we present a scheme outlining the new biochemical pathway of fatty acid and lipoic acid synthesis in plant mitochondria.
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Cited by
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Campbell, J. W., & Cronan, J. E. (2001) · Annual Review of Microbiology · doi:10.1146/annurev.micro.55.1.305 · Cited by 479
Li, J., Han, D., Wang, D., Ning, K., Jia, J., Wei, L., Jing, X., Huang, S., Chen, J., Li, Y., et al. (2014) · The Plant Cell · doi:10.1105/tpc.113.121418 · Cited by 412
Microbial Type I Fatty Acid Synthases (FAS): Major Players in a Network of Cellular FAS Systems
Schweizer, E., & Hofmann, J. (2004) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.68.3.501-517.2004 · Cited by 384
Li-Beisson, Y., Shorrosh, B., Beisson, F., Andersson, M. X., Arondel, V., Bates, P. D., Baud, S., Bird, D., DeBono, A., Durrett, T. P., et al. (2010) · The Arabidopsis Book · doi:10.1199/tab.0133 · Cited by 367
17B-hydroxysteroid dehydrogenases as acyl thioester metabolizing enzymes
Hiltunen, J. K., Kastaniotis, A. J., Autio, K. J., Jiang, G., Chen, Z., & Glumoff, T. (2019) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2018.11.012 · Cited by 43
Metadata
- Authors
- Gueguen, V., Macherel, D., Jaquinod, M., Douce, R., & Bourguignon, J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-02
- Volume
- 275
- Issue
- 7
- Pages
- 5016-5025
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2110958932
- Cited by
- 192
- References
- 0
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Gueguen, V., Macherel, D., Jaquinod, M., Douce, R., & Bourguignon, J. (2000). Fatty Acid and Lipoic Acid Biosynthesis in Higher Plant Mitochondria. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.275.7.5016
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
