The DOI matches its Crossref record.
An Escherichia coli Mutant Defective in Lipid Export
Open accessAbstract
Escherichia coli phospholipids and lipopolysaccharide, made on the inner surface of the inner membrane, are rapidly transported to the outer membrane by mechanisms that are not well characterized. We now report a temperature-sensitive mutant (WD2) with an A270T substitution in a trans-membrane region of the ABC transporter MsbA. As shown by (32)P(i) and (14)C-acetate labeling, export of all major lipids to the outer membrane is inhibited by approximately 90% in WD2 after 30 min at 44 degrees C. Transport of newly synthesized proteins is not impaired. Electron microscopy shows reduplicated inner membranes in WD2 at 44 degrees C, consistent with a key role for MsbA in lipid trafficking.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Raetz, C. R. H., & Whitfield, C. (2002) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.71.110601.135414 · Cited by 4,404
Self-citationMolecular Basis of Bacterial Outer Membrane Permeability Revisited
Nikaido, H. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.4.593-656.2003 · Cited by 4,316
Flexibility in the ABC transporter MsbA: Alternating access with a twist
Ward, A., Reyes, C. L., Yu, J., Roth, C. B., & Chang, G. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709388104 · Cited by 771
Biosynthesis and Export of Bacterial Lipopolysaccharides
Whitfield, C., & Trent, M. S. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035600 · Cited by 709
ATP-Binding Cassette Transporters in Bacteria
Davidson, A. L., & Chen, J. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073626 · Cited by 666
Metadata
- Authors
- Doerrler, W. T., Reedy, M. C., & Raetz, C. H.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-04
- Volume
- 276
- Issue
- 15
- Pages
- 11461-11464
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2037518670
- Cited by
- 215
- References
- 0
- FWCI
- 8.28
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: An Escherichia coli Mutant Defective in Lipid Export. https://app.cochat.ai/papers/10.1074/jbc.c100091200
- The paper
- Doerrler, W. T., Reedy, M. C., & Raetz, C. H. (2001). An Escherichia coli Mutant Defective in Lipid Export. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.c100091200
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
