The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
The Mammalian de Novo DNA Methyltransferases DNMT3A and DNMT3B Are Also DNA 5-Hydroxymethylcytosine Dehydroxymethylases
Open accessAbstract
For cytosine (C) demethylation of vertebrate DNA, it is known that the TET proteins could convert 5-methyl C (5-mC) to 5-hydroxymethyl C (5-hmC). However, DNA dehydroxymethylase(s), or enzymes able to directly convert 5-hmC to C, have been elusive. We present in vitro evidence that the mammalian de novo DNA methyltransferases DNMT3A and DNMT3B, but not the maintenance enzyme DNMT1, are also redox-dependent DNA dehydroxymethylases. Significantly, intactness of the C methylation catalytic sites of these de novo enzymes is also required for their 5-hmC dehydroxymethylation activity. That DNMT3A and DNMT3B function bidirectionally both as DNA methyltransferases and as dehydroxymethylases raises intriguing and new questions regarding the structural and functional aspects of these enzymes and their regulatory roles in the dynamic modifications of the vertebrate genomes during development, carcinogenesis, and gene regulation.
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Cites
Jaenisch, R., & Bird, A. (2003) · Nature Genetics · doi:10.1038/ng1089 · Cited by 6,325
Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1
Tahiliani, M., Koh, K. P., Shen, Y., Pastor, W. A., Bandukwala, H., Brudno, Y., Agarwal, S., Iyer, L. M., Liu, D. R., Aravind, L., et al. (2009) · Science · doi:10.1126/science.1170116 · Cited by 5,836
Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine
Ito, S., Shen, L., Dai, Q., Wu, S. C., Collins, L. B., Swenberg, J. A., He, C., & Zhang, Y. (2011) · Science · doi:10.1126/science.1210597 · Cited by 3,451
The Nuclear DNA Base 5-Hydroxymethylcytosine Is Present in Purkinje Neurons and the Brain
Kriaucionis, S., & Heintz, N. (2009) · Science · doi:10.1126/science.1169786 · Cited by 2,700
Induction of tumors in mice by genomic hypomethylation
Gaudet, F., Hodgson, J. G., Eden, A., Jackson-Grusby, L., Dausman, J., Gray, J. W., Leonhardt, H., & Jaenisch, R. (2003) · Science · doi:10.1126/science.1083558 · Cited by 1,499 · cited 2×
“The environment of the cancer cells, in particular the presence of oxidative stresses, likely would facilitate 5-mC oxidation (18, 34) as well as the dehydroxymethylase activities of DNMT3A/3B, thus leading to a profound reduction of the 5-hmC level (35–37) and also causing global hypomethylation of the cancer genomes…”
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Cited by
Reversing DNA Methylation: Mechanisms, Genomics, and Biological Functions
Wu, H., & Zhang, Y. (2014) · Cell · doi:10.1016/j.cell.2013.12.019 · Cited by 1,065
“First, removal of oxidized 5-position substituent, through either dehydroxymethylation of 5hmC by DNMTs (Chen et al., 2012; Liutkeviciute et al., 2009) or decarboxylation of 5caC by a putative decarboxylase (Schiesser et al., 2012; Wu and Zhang, 2010), may directly revert the oxidized 5mC base to…”
InfluentialTETonic shift: biological roles of TET proteins in DNA demethylation and transcription
Pastor, W. A., Aravind, L., & Rao, A. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3589 · Cited by 897
Genome-wide Analysis Reveals TET- and TDG-Dependent 5-Methylcytosine Oxidation Dynamics
Shen, L., Wu, H., Diep, D., Yamaguchi, S., D’Alessio, A. C., Fung, H.-L., Zhang, K., & Zhang, Y. (2013) · Cell · doi:10.1016/j.cell.2013.04.002 · Cited by 497
Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote
Guo, F., Li, X., Liang, D., Li, T., Zhu, P., Guo, H., Wu, X., Wen, L., Gu, T.-P., Hu, B., et al. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.08.003 · Cited by 375
Mechanism and Function of Oxidative Reversal of DNA and RNA Methylation
Shen, L., Song, C.-X., He, C., & Zhang, Y. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035513 · Cited by 358
Metadata
- Authors
- Chen, C.-C., Wang, K.-Y., & Shen, C.-K. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2012-09
- Volume
- 287
- Issue
- 40
- Pages
- 33116-33121
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2079161657
- Cited by
- 175
- References
- 5
- FWCI
- 9.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Chen, C.-C., Wang, K.-Y., & Shen, C.-K. J. (2012). The Mammalian de Novo DNA Methyltransferases DNMT3A and DNMT3B Are Also DNA 5-Hydroxymethylcytosine Dehydroxymethylases. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.c112.406975
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