The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Transcription-induced Cleavage of Immunoglobulin Switch Regions by Nucleotide Excision Repair Nucleases in Vitro
Open accessAbstract
Immunoglobulin (Ig) heavy chain class switch recombination (CSR) mediates isotype switching during B cell development. CSR occurs between switch (S) regions that precede each Ig heavy chain constant region gene. Various studies have demonstrated that transcription plays an essential role in CSR in vivo. In this study, we show that in vitro transcription of S regions in their physiological orientation induces the formation of stable R loops. Furthermore, we show that the nucleotide excision repair nucleases XPF-ERCC1 and XPG can cleave the R loops formed in the S regions. Based on these findings, we propose that CSR is initiated via a mechanism that involves transcription-dependent S region cleavage by DNA structure-specific endonucleases that function in general DNA repair processes. Such a mechanism also may underlie transcription-dependent mutagenic processes such as somatic hypermutation, and contribute to genomic instability in general.
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Sancar, A. (1996) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.65.070196.000355 · Cited by 1,131
Cited by
R-Loop Formation Is a Distinctive Characteristic of Unmethylated Human CpG Island Promoters
Ginno, P. A., Lott, P. L., Christensen, H. C., Korf, I., & Chédin, F. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.01.017 · Cited by 910
Huertas, P., & Aguilera, A. (2003) · Molecular Cell · doi:10.1016/j.molcel.2003.08.010 · Cited by 746
R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cells
Yu, K., Chedin, F., Hsieh, C.-L., Wilson, T. E., & Lieber, M. R. (2003) · Nature Immunology · doi:10.1038/ni919 · Cited by 746
Transcription-targeted DNA deamination by the AID antibody diversification enzyme
Chaudhuri, J., Tian, M., Khuong, C., Chua, K., Pinaud, E., & Alt, F. W. (2003) · Nature · doi:10.1038/nature01574 · Cited by 746
Self-citationThe Biology of IgE and the Basis of Allergic Disease
Gould, H. J., Sutton, B. J., Beavil, A. J., Beavil, R. L., McCloskey, N., Coker, H. A., Fear, D., & Smurthwaite, L. (2003) · Annual Review of Immunology · doi:10.1146/annurev.immunol.21.120601.141103 · Cited by 685
Metadata
- Authors
- Tian, M., & Alt, F. W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-08
- Volume
- 275
- Issue
- 31
- Pages
- 24163-24172
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2053183695
- Cited by
- 203
- References
- 1
- FWCI
- 5.07
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Tian, M., & Alt, F. W. (2000). Transcription-induced Cleavage of Immunoglobulin Switch Regions by Nucleotide Excision Repair Nucleases in Vitro. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m003343200
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
