The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Extracellular Glycosaminoglycans Modify Cellular Trafficking of Lipoplexes and Polyplexes
Open accessAbstract
It has been shown that extracellular glycosaminoglycans (GAGs) limit the gene transfer by cationic lipids and polymers. The purpose of this study was to clarify how interactions with anionic GAGs (hyaluronic acid and heparan sulfate) modify the cellular uptake and distribution of lipoplexes and polyplexes. Experiments on cellular DNA uptake and GFP reporter gene expression showed that decreased gene expression can rarely be explained by lower cellular uptake. In most cases, the cellular uptake is not changed by GAG binding to the lipoplexes or polyplexes. Reporter gene expression is decreased or blocked by heparan sulfate, but it is increased by hyaluronic acid; this suggests that intracellular factors are involved. Confocal microscopy experiments demonstrated that extracellular heparan sulfate and hyaluronic acid are taken into cells both with free and DNA-associated carriers. We conclude that extracellular GAGs may alter both the cellular uptake and the intracellular behavior of the DNA complexes.
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Cites
Direct Gene Transfer into Mouse Muscle in Vivo
Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L. (1990) · Science · doi:10.1126/science.1690918 · Cited by 4,173
Cited by
Formation and Intracellular Trafficking of Lipoplexes and Polyplexes
Elouahabi, A., & Ruysschaert, J.-M. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2004.12.006 · Cited by 462
The great escape: how cationic polyplexes overcome the endosomal barrier
Bus, T., Traeger, A., & Schubert, U. S. (2018) · Journal of Materials Chemistry B · doi:10.1039/c8tb00967h · Cited by 409
Polymeric Delivery of Therapeutic Nucleic Acids
Kumar, R., Santa Chalarca, C. F., Bockman, M. R., Bruggen, C. V., Grimme, C. J., Dalal, R. J., Hanson, M. G., Hexum, J. K., & Reineke, T. M. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00997 · Cited by 365
A model for non‐viral gene delivery: through syndecan adhesion molecules and powered by actin
Kopatz, I., Remy, J., & Behr, J. (2004) · The Journal of Gene Medicine · doi:10.1002/jgm.558 · Cited by 323
Pharmacokinetics and in vivo drug release rates in liposomal nanocarrier development
Drummond, D. C., Noble, C. O., Hayes, M. E., Park, J. W., & Kirpotin, D. B. (2008) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.21358 · Cited by 289
Metadata
- Authors
- Ruponen, M., Rönkkö, S., Honkakoski, P., Pelkonen, J., Tammi, M., & Urtti, A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-09
- Volume
- 276
- Issue
- 36
- Pages
- 33875-33880
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2050891199
- Cited by
- 206
- References
- 1
- FWCI
- 3.80
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Ruponen, M., Rönkkö, S., Honkakoski, P., Pelkonen, J., Tammi, M., & Urtti, A. (2001). Extracellular Glycosaminoglycans Modify Cellular Trafficking of Lipoplexes and Polyplexes. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m011553200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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