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Cited by 211

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Iron-Sulfur Cluster Assembly

Open access
Ollagnier-de-Choudens, Sandrine, Mattioli, Tony, Takahashi, Yasuhiro, Fontecave, Marc·2001·Journal of Biological Chemistry

Abstract

The synthesis of iron-sulfur clusters in Escherichia coli is believed to require a complex protein machinery encoded by the isc (iron-sulfur cluster) operon. The product of one member of this operon, IscA, has been overexpressed, purified, and characterized. It can assemble an air-sensitive [2Fe-2S] cluster as shown by UV-visible and resonance Raman spectroscopy. The metal form but not the apoform of IscA binds ferredoxin, another member of the isc operon, selectively, allowing transfer of iron and sulfide from IscA to ferredoxin and formation of the [2Fe-2S] holoferredoxin. These results thus suggest that IscA is involved in ferredoxin cluster assembly and activation. This is an important function because a functional ferredoxin is required for maturation of other cellular Fe-S proteins.

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Metadata

Authors
Ollagnier-de-Choudens, S., Mattioli, T., Takahashi, Y., & Fontecave, M.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-06
Volume
276
Issue
25
Pages
22604-22607
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2000959843
Cited by
211
References
2
FWCI
22.44
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Iron-Sulfur Cluster Assembly. https://app.cochat.ai/papers/10.1074/jbc.m102902200
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Ollagnier-de-Choudens, S., Mattioli, T., Takahashi, Y., & Fontecave, M. (2001). Iron-Sulfur Cluster Assembly. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m102902200

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.