The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Lipopolysaccharide-induced Expression of Interferon-β Mediates the Timing of Inducible Nitric-oxide Synthase Induction in RAW 264.7 Macrophages
Open accessAbstract
The production of nitric oxide by macrophages has been implicated as a host defense mechanism against microbial pathogens and tumor cells. Recent reports have implicated interferon-alpha/beta (IFN-alpha/beta) as an autocrine/paracrine signal critical for the induction of murine iNOS. In this report we have systematically investigated the role of IFN-beta in the induction of iNOS in the murine macrophage cell line, RAW 264.7. First, we demonstrate that IFN-beta expression is highly up-regulated, and is secreted in response to lipopolysaccharide (LPS). Treatment of RAW macrophages with LPS results in a time-dependent phosphorylation of STAT-1 on both tyrosine residue 701 (Tyr-701) and serine residue 727 (Ser-727) that is consistent with the timing of endogenous IFN-beta expression. LPS also induces interferon regulatory factor-1 expression with similar kinetics. We further demonstrate that exogenous IFN-beta accelerates the induction of iNOS by LPS. The acceleration of iNOS induction is observed at the levels of transcription, protein expression, and NO formation. Accordingly, we propose that the cytokine environment of macrophages may determine the rate and magnitude of nitric oxide production, thereby regulating the cytotoxic response to pathogen challenge.
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Wen, Z., Zhong, Z., & Darnell, J. E. (1995) · Cell · doi:10.1016/0092-8674(95)90311-9 · Cited by 1,974
The growing family of interferon regulatory factors
Nguyen, H., Hiscott, J., & Pitha, P. M. (1997) · Cytokine & Growth Factor Reviews · doi:10.1016/s1359-6101(97)00019-1 · Cited by 480
Stuehr, D. J., Gross, S. S., Sakuma, I., Levi, R., & Nathan, C. F. (1989) · The Journal of experimental medicine · doi:10.1084/jem.169.3.1011 · Cited by 457
Cited by
Essandoh, K., Yang, L., Wang, X., Huang, W., Qin, D., Hao, J., Wang, Y., Zingarelli, B., Peng, T., & Fan, G.-C. (2015) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2015.08.010 · Cited by 441
Nitric oxide function in the skin
Cals-Grierson, M.-M., & Ormerod, A. D. (2004) · Nitric Oxide · doi:10.1016/j.niox.2004.04.005 · Cited by 329
Species-specific Regulation of Toll-like Receptor 3 Genes in Men and Mice
Heinz, S., Haehnel, V., Karaghiosoff, M., Schwarzfischer, L., Müller, M., Krause, S. W., & Rehli, M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301476200 · Cited by 202
Cutting Edge: Nitric Oxide Inhibits the NLRP3 Inflammasome
Hernandez-Cuellar, E., Tsuchiya, K., Hara, H., Fang, R., Sakai, S., Kawamura, I., Akira, S., & Mitsuyama, M. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1202479 · Cited by 184
Kuchtey, J., Chefalo, P. J., Gray, R. C., Ramachandra, L., & Harding, C. V. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.4.2244 · Cited by 66
Metadata
- Authors
- Jacobs, A. T., & Ignarro, L. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-12
- Volume
- 276
- Issue
- 51
- Pages
- 47950-47957
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1578257252
- Cited by
- 197
- References
- 3
- FWCI
- 2.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Jacobs, A. T., & Ignarro, L. J. (2001). Lipopolysaccharide-induced Expression of Interferon-β Mediates the Timing of Inducible Nitric-oxide Synthase Induction in RAW 264.7 Macrophages. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m106639200
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