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Phosphatidylinositol-4-phosphate 5-kinases and phosphatidylinositol 4,5-bisphosphate synthesis in the brain

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Volpicelli-Daley, Laura A., Lucast, Louise, Gong, Liang-Wei, Liu, Lijuan, Sasaki, Junko, Sasaki, Takehiko, Abrams, Charles S., Kanaho, Yasunori +1 more·2010·Journal of Biological Chemistry
DOI 10.1074/jbc.m110.132191PMC PMC2937898
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Abstract

The predominant pathway for phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P(2)) synthesis is thought to be phosphorylation of phosphatidylinositol 4-phosphate at the 5 position of the inositol ring by type I phosphatidylinositol phosphate kinases (PIPK): PIPKIalpha, PIPKIbeta, and PIPKIgamma. PIPKIgamma has been shown to play a role in PI(4,5)P(2) synthesis in brain, and the absence of PIPKIgamma is incompatible with postnatal life. Conversely, mice lacking PIPKIalpha or PIPKIbeta (isoforms are referred to according to the nomenclature of human PIPKIs) live to adulthood, although functional effects in specific cell types are observed. To determine the contribution of PIPKIalpha and PIPKIbeta to PI(4,5)P(2) synthesis in brain, we investigated the impact of disrupting multiple PIPKI genes. Our results show that a single allele of PIPKIgamma, in the absence of both PIPKIalpha and PIPKIbeta, can support life to adulthood. In addition, PIPKIalpha alone, but not PIPKIbeta alone, can support prenatal development, indicating an essential and partially overlapping function of PIPKIalpha and PIPKIgamma during embryogenesis. This is consistent with early embryonic expression of PIPKIalpha and PIPKIgamma but not of PIPKIbeta. PIPKIbeta expression in brain correlates with neuronal differentiation. The absence of PIPKIbeta does not impact embryonic development in the PIPKIgamma knock-out (KO) background but worsens the early postnatal phenotype of the PIPKIgamma KO (death occurs within minutes rather than hours). Analysis of PIP(2) in brain reveals that only the absence of PIPKIgamma significantly impacts its levels. Collectively, our results provide new evidence for the dominant importance of PIPKIgamma in mammals and imply that PIPKIalpha and PIPKIbeta function in the generation of specific PI(4,5)P(2) pools that, at least in brain, do not have a major impact on overall PI(4,5)P(2) levels.

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  1. [1]Hokin M. R. Hokin L. E. (1953) J. Biol. Chem. 203, 967– 977 13084667
  2. [2]Di Paolo G. De Camilli P. (2006) Nature 443, 651– 657 17035995
  3. [3]Heck J. N. Mellman D. L. Ling K. Sun Y. Wagoner M. P. Schill N. J. Anderson R. A. (2007) Crit. Rev. Biochem. Mol. Biol. 42, 15– 39 17364683
  4. [4]Mao Y. S. Yin H. L. (2007) Pflugers Arch. 455, 5– 18 17520274
  5. [5]Barlow C. A. Laishram R. S. Anderson R. A. (2010) Trends Cell Biol. 20, 25– 35 19846310 PMC2818233
  6. [6]van den Bout I. Divecha N. (2009) J. Cell Sci. 122, 3837– 3850 19889969
  7. [7]Voronov S. V. Frere S. G. Giovedi S. Pollina E. A. Borel C. Zhang H. Schmidt C. Akeson E. C. Wenk M. R. Cimasoni L. Arancio O. Davisson M. T. Antonarakis S. E. Gardiner K. De Camilli P. Di Paolo G. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 9415– 9420 18591654 PMC2453748
  8. [8]Attree O. Olivos I. M. Okabe I. Bailey L. C. Nelson D. L. Lewis R. A. McInnes R. R. Nussbaum R. L. (1992) Nature 358, 239– 242 1321346
  9. [9]Schurman S. J. Scheinman S. J. (2009) Nat. Rev. Nephrol. 5, 529– 538 19701229
  10. [10]McCrea H. J. De Camilli P. (2009) Physiology 24, 8– 16 19196647 PMC3499097
  11. [11]Saito T. Stopkova P. Diaz L. Papolos D. F. Boussemart L. Lachman H. M. (2003) Am. J. Med. Genet. B Neuropsychiatr Genet. 116B, 77– 83 12497619
  12. [12]Stopkova P. Saito T. Fann C. S. Papolos D. F. Vevera J. Paclt I. Zukov I. Stryjer R. Strous R. D. Lachman H. M. (2003) Am. J. Med. Genet. B. Neuropsychiatr. Genet. 123B, 50– 58 14582145
  13. [13]Soares J. C. Dippold C. S. Wells K. F. Frank E. Kupfer D. J. Mallinger A. G. (2001) Neurosci. Lett. 299, 150– 152 11166959
  14. [14]Landman N. Jeong S. Y. Shin S. Y. Voronov S. V. Serban G. Kang M. S. Park M. K. Di Paolo G. Chung S. Kim T. W. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 19524– 19529 17158800 PMC1748258
  15. [15]Berman D. E. Dall'Armi C. Voronov S. V. McIntire L. B. Zhang H. Moore A. Z. Staniszewski A. Arancio O. Kim T. W. Di Paolo G. (2008) Nat. Neurosci. 11, 547– 554 18391946 PMC2532986
  16. [16]Marion E. Kaisaki P. J. Pouillon V. Gueydan C. Levy J. C. Bodson A. Krzentowski G. Daubresse J. C. Mockel J. Behrends J. Servais G. Szpirer C. Kruys V. Gauguier D. Schurmans S. (2002) Diabetes 51, 2012– 2017 12086927
  17. [17]Yuan T. L. Cantley L. C. (2008) Oncogene 27, 5497– 5510 18794884 PMC3398461
  18. [18]Jacoby M. Cox J. J. Gayral S. Hampshire D. J. Ayub M. Blockmans M. Pernot E. Kisseleva M. V. Compère P. Schiffmann S. N. Gergely F. Riley J. H. Pérez-Morga D. Woods C. G. Schurmans S. (2009) Nat. Genet. 41, 1027– 1031 19668215
  19. [19]Bielas S. L. Silhavy J. L. Brancati F. Kisseleva M. V. Al-Gazali L. Sztriha L. Bayoumi R. A. Zaki M. S. Abdel-Aleem A. Rosti R. O. Kayserili H. Swistun D. Scott L. C. Bertini E. Boltshauser E. Fazzi E. Travaglini L. Field S. J. Gayral S. Jacoby M. Schurmans S. Dallapiccola B. Majerus P. W. Valente E. M. Gleeson J. G. (2009) Nat. Genet. 41, 1032– 1036 19668216 PMC2746682
  20. [20]Norris F. A. Wilson M. P. Wallis T. S. Galyov E. E. Majerus P. W. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 14057– 14059 9826652 PMC24325
  21. [21]Terebiznik M. R. Vieira O. V. Marcus S. L. Slade A. Yip C. M. Trimble W. S. Meyer T. Finlay B. B. Grinstein S. (2002) Nat. Cell Biol. 4, 766– 773 12360287
  22. [22]McLaughlin S. Murray D. (2005) Nature 438, 605– 611 16319880
  23. [23]Krauss M. Haucke V. (2007) EMBO Rep. 8, 241– 246 17330069 PMC1808040
  24. [24]Rameh L. E. Tolias K. F. Duckworth B. C. Cantley L. C. (1997) Nature 390, 192– 196 9367159
  25. [25]Ishihara H. Shibasaki Y. Kizuki N. Katagiri H. Yazaki Y. Asano T. Oka Y. (1996) J. Biol. Chem. 271, 23611– 23614 8798574
  26. [26]Loijens J. C. Anderson R. A. (1996) J. Biol. Chem. 271, 32937– 32943 8955136
  27. [27]Chang J. D. Field S. J. Rameh L. E. Carpenter C. L. Cantley L. C. (2004) J. Biol. Chem. 279, 11672– 11679 14701839
  28. [28]Bairstow S. F. Ling K. Su X. Firestone A. J. Carbonara C. Anderson R. A. (2006) J. Biol. Chem. 281, 20632– 20642 16707488
  29. [29]Nakano-Kobayashi A. Yamazaki M. Unoki T. Hongu T. Murata C. Taguchi R. Katada T. Frohman M. A. Yokozeki T. Kanaho Y. (2007) EMBO J. 26, 1105– 1116 17290217 PMC1852847
  30. [30]Krauss M. Kukhtina V. Pechstein A. Haucke V. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 11934– 11939 16880396 PMC1567676
  31. [31]Thieman J. R. Mishra S. K. Ling K. Doray B. Anderson R. A. Traub L. M. (2009) J. Biol. Chem. 284, 13924– 13939 19287005 PMC2679492
  32. [32]Di Paolo G. Moskowitz H. S. Gipson K. Wenk M. R. Voronov S. Obayashi M. Flavell R. Fitzsimonds R. M. Ryan T. A. De Camilli P. (2004) Nature 431, 415– 422 15386003
  33. [33]Ling K. Doughman R. L. Firestone A. J. Bunce M. W. Anderson R. A. (2002) Nature 420, 89– 93 12422220
  34. [34]Di Paolo G. Pellegrini L. Letinic K. Cestra G. Zoncu R. Voronov S. Chang S. Guo J. Wenk M. R. De Camilli P. (2002) Nature 420, 85– 89 12422219
  35. [35]Fairn G. D. Ogata K. Botelho R. J. Stahl P. D. Anderson R. A. De Camilli P. Meyer T. Wodak S. Grinstein S. (2009) J. Cell Biol. 187, 701– 714 19951917 PMC2806594
  36. [36]Min S. H. Abrams C. S. (2009) Biochem. J. 423, e5– 8 19740077
  37. [37]Desrivières S. Cooke F. T. Parker P. J. Hall M. N. (1998) J. Biol. Chem. 273, 15787– 15793 9624178
  38. [38]Wenk M. R. Pellegrini L. Klenchin V. A. Di Paolo G. Chang S. Daniell L. Arioka M. Martin T. F. De Camilli P. (2001) Neuron 32, 79– 88 11604140
  39. [39]Ishihara H. Shibasaki Y. Kizuki N. Wada T. Yazaki Y. Asano T. Oka Y. (1998) J. Biol. Chem. 273, 8741– 8748 9535851
  40. [40]Mao Y. S. Yamaga M. Zhu X. Wei Y. Sun H. Q. Wang J. Yun M. Wang Y. Di Paolo G. Bennett M. Mellman I. Abrams C. S. De Camilli P. Lu C. Y. Yin H. L. (2009) J. Cell Biol. 184, 281– 296 19153220 PMC2654300
  41. [41]Lacalle R. A. Peregil R. M. Albar J. P. Merino E. Martínez-A. C. Mérida I. Mañes S. (2007) J. Cell Biol. 179, 1539– 1553 18158329 PMC2373511
  42. [42]Vasudevan L. Jeromin A. Volpicelli-Daley L. De Camilli P. Holowka D. Baird B. (2009) J. Cell Sci. 122, 2567– 2574 19549683 PMC2704887
  43. [43]Wang Y. Lian L. Golden J. A. Morrisey E. E. Abrams C. S. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 11748– 11753 17609388 PMC1913884
  44. [44]Narkis G. Ofir R. Landau D. Manor E. Volokita M. Hershkowitz R. Elbedour K. Birk O. S. (2007) Am. J. Hum. Genet. 81, 530– 539 17701898 PMC1950840
  45. [45]Wang Y. Chen X. Lian L. Tang T. Stalker T. J. Sasaki T. Kanaho Y. Brass L. F. Choi J. K. Hartwig J. H. Abrams C. S. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 14064– 14069 18772378 PMC2544579
  46. [46]Sasaki J. Sasaki T. Yamazaki M. Matsuoka K. Taya C. Shitara H. Takasuga S. Nishio M. Mizuno K. Wada T. Miyazaki H. Watanabe H. Iizuka R. Kubo S. Murata S. Chiba T. Maehama T. Hamada K. Kishimoto H. Frohman M. A. Tanaka K. Penninger J. M. Yonekawa H. Suzuki A. Kanaho Y. (2005) J. Exp. Med. 201, 859– 870 15767368 PMC2213097
  47. [47]Cremona O. Di Paolo G. Wenk M. R. Lüthi A. Kim W. T. Takei K. Daniell L. Nemoto Y. Shears S. B. Flavell R. A. McCormick D. A. De Camilli P. (1999) Cell 99, 179– 188 10535736
  48. [48]Erdmann K. S. Mao Y. McCrea H. J. Zoncu R. Lee S. Paradise S. Modregger J. Biemesderfer D. Toomre D. De Camilli P. (2007) Dev. Cell 13, 377– 390 17765681 PMC2025683
  49. [49]Davis J. N. Rock C. O. Cheng M. Watson J. B. Ashmun R. A. Kirk H. Kay R. J. Roussel M. F. (1997) Mol. Cell. Biol. 17, 7398– 7406 9372970 PMC232595
  50. [50]Nasuhoglu C. Feng S. Mao J. Yamamoto M. Yin H. L. Earnest S. Barylko B. Albanesi J. P. Hilgemann D. W. (2002) Anal. Biochem. 301, 243– 254 11814295
  51. [51]Stevens C. F. Tsujimoto T. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 846– 849 7846064 PMC42717
  52. [52]Padrón D. Wang Y. J. Yamamoto M. Yin H. Roth M. G. (2003) J. Cell Biol. 162, 693– 701 12913109 PMC2173809
  53. [53]Ooms L. M. Horan K. A. Rahman P. Seaton G. Gurung R. Kethesparan D. S. Mitchell C. A. (2009) Biochem. J. 419, 29– 49 19272022
  54. [54]Parker G. J. Loijens J. C. Anderson R. A. (1998) Methods Mol. Biol. 105, 127– 139 10427556
  55. [55]Wenk M. R. Lucast L. Di Paolo G. Romanelli A. J. Suchy S. F. Nussbaum R. L. Cline G. W. Shulman G. I. McMurray W. De Camilli P. (2003) Nat. Biotechnol. 21, 813– 817 12808461
  56. [56]Pan W. Choi S. C. Wang H. Qin Y. Volpicelli-Daley L. Swan L. Lucast L. Khoo C. Zhang X. Li L. Abrams C. S. Sokol S. Y. Wu D. (2008) Science 321, 1350– 1353 18772438 PMC2532521
  57. [57]Mellman D. L. Gonzales M. L. Song C. Barlow C. A. Wang P. Kendziorski C. Anderson R. A. (2008) Nature 451, 1013– 1017 18288197
  58. [58]Paquet M. Kuwajima M. Yun C. C. Smith Y. Hall R. A. (2006) J. Comp. Neurol. 494, 752– 762 16374813 PMC1472808
  59. [59]Hernández-Deviez D. J. Roth M. G. Casanova J. E. Wilson J. M. (2004) Mol. Biol. Cell 15, 111– 120 14565977 PMC307532
  60. [60]Yamazaki M. Miyazaki H. Watanabe H. Sasaki T. Maehama T. Frohman M. A. Kanaho Y. (2002) J. Biol. Chem. 277, 17226– 17230 11877391
  61. [61]Ling K. Bairstow S. F. Carbonara C. Turbin D. A. Huntsman D. G. Anderson R. A. (2007) J. Cell Biol. 176, 343– 353 17261850 PMC2063960
  62. [62]Lee S. Y. Voronov S. Letinic K. Nairn A. C. Di Paolo G. De Camilli P. (2005) J. Cell Biol. 168, 789– 799 15738269 PMC2171813
  63. [63]Wang Y. J. Li W. H. Wang J. Xu K. Dong P. Luo X. Yin H. L. (2004) J. Cell Biol. 167, 1005– 1010 15611330 PMC2172614

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Authors
Volpicelli-Daley, L. A., Lucast, L., Gong, L.-W., Liu, L., Sasaki, J., Sasaki, T., Abrams, C. S., Kanaho, Y., & De Camilli, P.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Akita University (JP)
  • University of Pennsylvania (US)
  • University of Tsukuba (JP)
ORCID
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2010-09
Volume
285
Issue
37
Pages
28708-28714
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m110.132191
PMC
PMC2937898
OpenAlex
W1982532032
Cited by
57
References
1
Citations by year
2026: 2 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 3 · 2020: 3 · 2019: 4 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 5 · 2014: 6 · 2013: 8 · 2012: 8
FWCI
2.66
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • Japan Society for the Promotion of Science (21200075)
  • National Institute on Drug Abuse (DA 018343, P30DA018343)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK 45735, P30DK045735)
  • National Institute of Neurological Disorders and Stroke (R01NS036251, NS36251, R37NS036251)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 4-phosphate 5-kinase, phosphatidylinositol phosphate kinase, brain, embryonic development, postnatal lethality, neuronal differentiation, knockout mice
MeSH terms
Animals, Brain, Brain Chemistry, Cell Differentiation, Embryo, Mammalian, Humans, Neurons, Mice, Knockout, Phosphatidylinositol 4,5-Diphosphate, Phosphatidylinositol 3-Kinases, Embryonic Development, Mice

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