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Cited by 15Retinoids in leukemia and cellular processesPeroxisome Proliferator-Activated ReceptorsNuclear Receptors and Signaling

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A Novel Cytoplasmic Adaptor for Retinoic Acid Receptor (RAR) and Thyroid Receptor Functions as a Derepressor of RAR in the Absence of Retinoic Acid*

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Park, Ui-Hyun, Kim, Eun-Joo, Um, Soo-Jong·2010·Journal of Biological Chemistry
DOI 10.1074/jbc.m110.143008PMC PMC2962525
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Abstract

In most mammalian cells, the retinoic acid receptor (RAR) is nuclear rather than cytoplasmic, regardless of its cognate ligand, retinoic acid (RA). In testis Sertoli cells, however, RAR is retained in the cytoplasm and moves to the nucleus only when RA is supplied. This led us to identify a protein that regulates the translocation of RAR. From yeast two-hybrid screening, we identified a novel RAR-interacting protein called CART1 (cytoplasmic adaptor for RAR and TR). Systematic interaction assays using deletion mutants showed that the C-terminal CoRNR box of CART1 was responsible for the interaction with the NCoR binding region of RAR and TR. Such interaction was impaired in the presence of ligand RA, as further determined by GST pulldown assays in vitro and immunoprecipitation assays in vivo. Fluorescence microscopy showed that unliganded RAR was captured by CART1 in the cytoplasm, whereas liganded RAR was liberated and moved to the nucleus. Overexpression of CART1 blocked the transcriptional repressing activity of unliganded apoRAR, mediated by corepressor NCoR in the nucleus. CART1 siRNA treatment in a mouse Sertoli cell line, TM4, allowed RAR to move to the nucleus and blocked the derepressing function of CART1, suggesting that CART1 might be a cytoplasmic, testis-specific derepressor of RAR.

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Metadata

Authors
Park, U.-H., Kim, E.-J., & Um, S.-J.
Affiliations
Sejong University (KR)
Corresponding author
Um, Soo-Jong
ORCID
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2010-10
Volume
285
Issue
44
Pages
34269-34278
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m110.143008
PMC
PMC2962525
OpenAlex
W2058958130
Cited by
15
References
1
Citations by year
2025: 1 · 2023: 1 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 4 · 2016: 1 · 2013: 2 · 2012: 1
FWCI
0.52
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Receptors and Signaling
Keywords
retinoic acid receptor, thyroid hormone receptor, retinoic acid, nuclear translocation, transcriptional derepression, Sertoli cells, testis-specific proteins, protein-protein interaction
MeSH terms
Animals, Cell Nucleus, Cytoplasm, Gene Expression Regulation, Humans, Male, Microscopy, Fluorescence, Receptors, Thyroid Hormone, Sertoli Cells, Testis, Transcription Factors, Tretinoin, Receptors, Retinoic Acid, Two-Hybrid System Techniques, NIH 3T3 Cells, Mice

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CoChat. (2026). Citation check: A Novel Cytoplasmic Adaptor for Retinoic Acid Receptor (RAR) and Thyroid Receptor Functions as a Derepressor of RAR in the Absence of Retinoic Acid*. https://app.cochat.ai/papers/10.1074/jbc.m110.143008
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Park, U.-H., Kim, E.-J., & Um, S.-J. (2010). A Novel Cytoplasmic Adaptor for Retinoic Acid Receptor (RAR) and Thyroid Receptor Functions as a Derepressor of RAR in the Absence of Retinoic Acid*. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m110.143008