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Cited by 29Neuroscience and Neuropharmacology ResearchReceptor Mechanisms and SignalingAmino Acid Enzymes and Metabolism

Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes

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Kang, Myoung-Goo, Nuriya, Mutsuo, Guo, Yurong, Martindale, Kevin D., Lee, Daniel Z., Huganir, Richard L.·2012·Journal of Biological Chemistry
DOI 10.1074/jbc.m111.336644PMC PMC3436506
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Abstract

The AMPA receptor (AMPA-R) is a major excitatory neurotransmitter receptor in the brain. Identifying and characterizing the neuronal proteins interacting with AMPA-Rs have provided important information about the molecular mechanisms underlying synaptic transmission and plasticity. In this study, to identify more AMPA-R interactors in vivo, we performed proteomic analyses of AMPA-R complexes from the brain. AMPA-R complexes were isolated from the brain through various combinations of biochemical techniques for solubilization, enrichment, and immunoprecipitation. Mass spectrometry analyses of these isolated complexes identified several novel components of the AMPA-R complexes as well as some previously identified components. The identification of these novel components helps to further define the complex mechanisms involved in the regulation of AMPA receptor function and synaptic plasticity.

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Authors
Kang, M.-G., Nuriya, M., Guo, Y., Martindale, K. D., Lee, D. Z., & Huganir, R. L.
Affiliations
  • Howard Hughes Medical Institute (US)
  • University of California San Diego (US)
  • The University of Texas Medical Branch at Galveston (US)
Corresponding author
  • Kang, Myoung-Goo
  • Huganir, Richard L.
ORCID
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2012-08
Volume
287
Issue
34
Pages
28632-28645
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m111.336644
PMC
PMC3436506
OpenAlex
W1963726213
Cited by
29
References
5
Citations by year
2025: 1 · 2024: 1 · 2022: 1 · 2021: 3 · 2020: 4 · 2018: 3 · 2017: 5 · 2016: 1 · 2015: 4 · 2014: 4 · 2013: 2
FWCI
1.32
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health (n01hv28180-13-0-0)
  • National Heart, Lung, and Blood Institute (N01 HV-28180, N01 HV028180)
  • National Institute of Neurological Disorders and Stroke (R01NS036715)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
Keywords
AMPA receptors, receptor complex, protein interactors, synaptic plasticity, brain, receptor regulation, synaptic transmission
MeSH terms
Animals, Synaptic Transmission, Receptors, AMPA, Proteomics, Multiprotein Complexes, Rats

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