Cited by 24Nitric Oxide and Endothelin EffectsProtein Kinase Regulation and GTPase SignalingReceptor Mechanisms and Signaling

Phosphorylation Controls Endothelial Nitric-oxide Synthase by Regulating Its Conformational Dynamics

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Haque, Mohammad Mahfuzul, Ray, Sougata Sinha, Stuehr, Dennis J.·2016·Journal of Biological Chemistry

Abstract

phosphorylation regulates eNOS and provides a foundation to compare its regulation by other phosphorylation events.

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Crossref · 10.1074/jbc.m116.737361

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58 of 58 references verified0 citation errors · 0 not checkable

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  • Ser1177 is located in the C-terminal portion of eNOS in a helical C-terminal tail element that blocks flavin reduction and regulates the conformational equilibrium of the reductase domain.

    “Ser1177 is located in the C-terminal portion of eNOS in a helical C-terminal tail element that blocks flavin reduction and regulates the conformational equilibrium of the reductase domain”
  • Ser1177 phosphorylation activates electron flow through the reductase domain and increases NO synthesis in functional eNOS.

    “Ser1177 phosphorylation activates electron flow through the reductase domain and, hence, increases NO synthesis in functional eNOS.”

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Authors
Haque, M. M., Ray, S. S., & Stuehr, D. J.
Affiliations
Cleveland Clinic (US)
Corresponding author
  • Haque, Mohammad Mahfuzul
  • Stuehr, Dennis J.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2016-10
Volume
291
Issue
44
Pages
23047-23057
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2513555323
Cited by
24
References
58
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 3 · 2018: 6 · 2017: 3
FWCI
3.22
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (GM051491, HL076491)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
Keywords
endothelial nitric oxide synthase, phosphorylation, protein conformational dynamics, enzyme regulation, post-translational modification
MeSH terms
Animals, Cattle, Flavins, Kinetics, Nitric Oxide, Phosphorylation, Protein Conformation, Amino Acid Substitution, Amino Acid Motifs, Cytochromes c, Nitric Oxide Synthase Type III

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