The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Accurate Translation of the Genetic Code Depends on tRNA Modified Nucleosides
Open accessAbstract
Transfer RNA molecules translate the genetic code by recognizing cognate mRNA codons during protein synthesis. The anticodon wobble at position 34 and the nucleotide immediately 3' to the anticodon triplet at position 37 display a large diversity of modified nucleosides in the tRNAs of all organisms. We show that tRNA species translating 2-fold degenerate codons require a modified U(34) to enable recognition of their cognate codons ending in A or G but restrict reading of noncognate or near-cognate codons ending in U and C that specify a different amino acid. In particular, the nucleoside modifications 2-thiouridine at position 34 (s(2)U(34)), 5-methylaminomethyluridine at position 34 (mnm(5)U(34)), and 6-threonylcarbamoyladenosine at position 37 (t(6)A(37)) were essential for Watson-Crick (AAA) and wobble (AAG) cognate codon recognition by tRNA(UUU)(Lys) at the ribosomal aminoacyl and peptidyl sites but did not enable the recognition of the asparagine codons (AAU and AAC). We conclude that modified nucleosides evolved to modulate an anticodon domain structure necessary for many tRNA species to accurately translate the genetic code.
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Cites
Crystal structure of the ribosome at 5.5 Å resolution
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Brodersen, D. E., Clemons, W. M., Carter, A. P., Morgan-Warren, R. J., Wimberly, B. T., & Ramakrishnan, V. (2000) · Cell · doi:10.1016/s0092-8674(00)00216-6 · Cited by 950
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Cited by
tRNA’s Wobble Decoding of the Genome: 40 Years of Modification
Agris, P. F., Vendeix, F. A. P., & Graham, W. D. (2007) · Journal of Molecular Biology · doi:10.1016/j.jmb.2006.11.046 · Cited by 543
Self-citationBjörk, G. R., & Hagervall, T. G. (2005) · EcoSal Plus · doi:10.1128/ecosalplus.4.6.2 · Cited by 340
The last universal common ancestor between ancient Earth chemistry and the onset of genetics
Weiss, M. C., Preiner, M., Xavier, J. C., Zimorski, V., & Martin, W. F. (2018) · PLOS Genetics · doi:10.1371/journal.pgen.1007518 · Cited by 186
Driving change: the evolution of alternative genetic codes
Santos, M. A. S., Moura, G., Massey, S. E., & Tuite, M. F. (2004) · Trends in Genetics · doi:10.1016/j.tig.2003.12.009 · Cited by 149
Rios, A. C., & Tor, Y. (2013) · Israel Journal of Chemistry · doi:10.1002/ijch.201300009 · Cited by 98
Metadata
- Authors
- Yarian, C., Townsend, H., Czestkowski, W., Sochacka, E., Malkiewicz, A. J., Guenther, R., Miskiewicz, A., & Agris, P. F.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2002-05
- Volume
- 277
- Issue
- 19
- Pages
- 16391-16395
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2016906651
- Cited by
- 248
- References
- 4
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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