The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
The Small Heat Shock Protein αB-crystallin Negatively Regulates Apoptosis during Myogenic Differentiation by Inhibiting Caspase-3 Activation
Open accessAbstract
Myoblasts respond to growth factor deprivation either by differentiating into multinucleated myotubes or by undergoing apoptosis; hence, the acquisition of apoptosis resistance by myogenic precursors is essential for their development. Here we demonstrate that the expression of the small heat shock protein alpha B-crystallin is selectively induced in C2C12 myoblasts that are resistant to differentiation-induced apoptosis, and we show that this induction occurs at an early stage in their differentiation in vitro. In contrast, the expression of several known anti-apoptotic proteins (FLIP, XIAP, Bcl-x(L)) was not altered during myogenesis. We also demonstrate that ectopic expression of alpha B-crystallin, but not the closely related small heat shock protein Hsp27, renders C2C12 myoblasts resistant to differentiation-induced apoptosis. Furthermore, we show that the myopathy-causing R120G alpha B-crystallin mutant is partly impaired in its cytoprotective function, whereas a pseudophosphorylation alpha B-crystallin mutant that mimics stress-induced phosphorylation is completely devoid of anti-apoptotic activity. Finally, we demonstrate that alpha B-crystallin negatively regulates apoptosis during myogenesis by inhibiting the proteolytic activation of caspase-3, whereas the R120G and pseudophosphorylation mutants are defective in this function. Taken together, our findings indicate that alpha B-crystallin is a novel negative regulator of myogenic apoptosis that directly links the differentiation program to apoptosis resistance.
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Self-citationCorrelation of Terminal Cell Cycle Arrest of Skeletal Muscle with Induction of p21 by MyoD
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A missense mutation in the αB-crystallin chaperone gene causes a desmin-related myopathy
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p53-Independent Expression of p21 Cip1 in Muscle and Other Terminally Differentiating Cells
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Hsp27 negatively regulates cell death by interacting with cytochrome c
Bruey, J.-M., Ducasse, C., Bonniaud, P., Ravagnan, L., Susin, S. A., Diaz-Latoud, C., Gurbuxani, S., Arrigo, A.-P., Kroemer, G., Solary, E., et al. (2000) · Nature Cell Biology · doi:10.1038/35023595 · Cited by 985
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Resistance to Apoptosis Conferred by Cdk Inhibitors During Myocyte Differentiation
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Kamradt, M. C., Chen, F., & Cryns, V. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.c100107200 · Cited by 356
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Caspase cleavage of tau: Linking amyloid and neurofibrillary tangles in Alzheimer's disease
Gamblin, T. C., Chen, F., Zambrano, A., Abraha, A., Lagalwar, S., Guillozet, A. L., Lu, M., Fu, Y., Garcia-Sierra, F., LaPointe, N., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1630428100 · Cited by 831
Self-citationBAG3 Deficiency Results in Fulminant Myopathy and Early Lethality
Homma, S., Iwasaki, M., Shelton, G. D., Engvall, E., Reed, J. C., & Takayama, S. (2006) · The American Journal of Pathology · doi:10.2353/ajpath.2006.060250 · Cited by 243
Metadata
- Authors
- Kamradt, M. C., Chen, F., Sam, S., & Cryns, V. L.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2002-10
- Volume
- 277
- Issue
- 41
- Pages
- 38731-38736
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2148982122
- Cited by
- 256
- References
- 31
- FWCI
- 6.26
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kamradt, M. C., Chen, F., Sam, S., & Cryns, V. L. (2002). The Small Heat Shock Protein αB-crystallin Negatively Regulates Apoptosis during Myogenic Differentiation by Inhibiting Caspase-3 Activation. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m201770200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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