The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Biochemistry of Mitochondrial Nitric-oxide Synthase
Open accessAbstract
We reported that the generation of nitric oxide by mitochondria is catalyzed by a constitutive, mitochondrial nitric-oxide synthase (mtNOS). Given that this production may establish the basis for a novel regulatory pathway of energy metabolism, oxygen consumption, and oxygen free radical production, it becomes imperative to identify unequivocally and characterize this enzyme to provide a basis for its regulation. The mitochondrial localization of mtNOS was supported by following the hepatic distribution of mtNOS, immunoblotting submitochondrial fractions, and immunohistochemistry of liver tissues. mtNOS was identified as brain NOS alpha by various methods (mass spectrometry of proteolytic fragments, amino acid analysis, molecular weight, pI, and analysis of PCR fragments), excluding the occurrence of a novel isoform or other splice variants. Distribution of mtNOS transcript indicated its occurrence in liver, brain, heart, muscle, kidney, lung, testis, and spleen. In contrast to brain NOS, mtNOS has two post-translational modifications: acylation with myristic acid and phosphorylation at the C terminus. The former modification is a reversible and post-translational process, which may serve for subcellular targeting or membrane anchoring. The latter modification could be linked to enzymatic regulation. These results are discussed in terms of the role that nitric oxide may have in cellular bioenergetics.
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Cited by
The mitochondrial energy transduction system and the aging process
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Mitochondrial nitric oxide synthase
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THE REGULATION AND PHARMACOLOGY OF ENDOTHELIAL NITRIC OXIDE SYNTHASE
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Metadata
- Authors
- Elfering, S. L., Sarkela, T. M., & Giulivi, C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2002-10
- Volume
- 277
- Issue
- 41
- Pages
- 38079-38086
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1977328014
- Cited by
- 347
- References
- 50
- FWCI
- 20.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Elfering, S. L., Sarkela, T. M., & Giulivi, C. (2002). Biochemistry of Mitochondrial Nitric-oxide Synthase. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m205256200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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