The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Sulfated Polysaccharides Enhance the Biological Activities of Bone Morphogenetic Proteins
Open accessAbstract
Bone morphogenetic proteins (BMPs), which have been shown to be heparin-binding proteins, induce osteoblast differentiation in mesenchymal cells. In the present study, we examined the effects of heparin on the BMP activities in C2C12 myoblasts. Heparin dose dependently enhanced the osteoblast differentiation induced by not only homodimers of BMP-2 or BMP-4 but also heterodimers of BMP-2/6 or BMP-2/7. However, the osteoblast differentiation induced by the constitutively active BMPR-IA, a functional BMP type I receptor, was not affected by heparin. Heparan sulfate and dextran sulfate also enhanced the BMP-2 activity, although the chemically desulfated heparin-derivatives have lost this stimulatory capacity. Heparin dose-dependently suppressed the accumulation of BMP-2 from the culture media into the cell layer or BMPR-IA, and retained a large amount of BMP-2 in the culture media. The biological activity of BMP-2, which was evaluated using a BMP-responsive reporter gene expression, was prolonged in the presence of heparin. Taken together, these results suggest that sulfated polysaccharides enhance the biological activity of both homodimers and heterodimers of BMPs by continuously serving the ligands to their signaling receptors expressed on cell membranes.
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Cites
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Shi, L. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.06.100 · Cited by 559
Design of artificial extracellular matrices for tissue engineering
Kim, B.-S., Park, I.-K., Hoshiba, T., Jiang, H.-L., Choi, Y.-J., Akaike, T., & Cho, C.-S. (2011) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2010.10.001 · Cited by 296
Polysaccharide biomaterials for drug delivery and regenerative engineering
Shelke, N. B., James, R., Laurencin, C. T., & Kumbar, S. G. (2014) · Polymers for Advanced Technologies · doi:10.1002/pat.3266 · Cited by 281
Lee, S. S., Huang, B. J., Kaltz, S. R., Sur, S., Newcomb, C. J., Stock, S. R., Shah, R. N., & Stupp, S. I. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2012.10.005 · Cited by 267
Metadata
- Authors
- Takada, T., Katagiri, T., Ifuku, M., Morimura, N., Kobayashi, M., Hasegawa, K., Ogamo, A., & Kamijo, R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-10
- Volume
- 278
- Issue
- 44
- Pages
- 43229-43235
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2015574865
- Cited by
- 270
- References
- 50
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Takada, T., Katagiri, T., Ifuku, M., Morimura, N., Kobayashi, M., Hasegawa, K., Ogamo, A., & Kamijo, R. (2003). Sulfated Polysaccharides Enhance the Biological Activities of Bone Morphogenetic Proteins. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m300937200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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