The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Kinetic Mechanism of Non-muscle Myosin IIB
Open accessAbstract
Besides driving contraction of various types of muscle tissue, conventional (class II) myosins serve essential cellular functions and are ubiquitously expressed in eukaryotic cells. Three different isoforms in the human myosin complement have been identified as non-muscle class II myosins. Here we report the kinetic characterization of a human non-muscle myosin IIB subfragment-1 construct produced in the baculovirus expression system. Transient kinetic data show that most steps of the actomyosin ATPase cycle are slowed down compared with other class II myosins. The ADP affinity of subfragment-1 is unusually high even in the presence of actin filaments, and the rate of ADP release is close to the steady-state ATPase rate. Thus, non-muscle myosin IIB subfragment-1 spends a significantly higher proportion of its kinetic cycle strongly attached to actin than do the muscle myosins. This feature is even more pronounced at slightly elevated ADP levels, and it may be important in carrying out the cellular functions of this isoform working in small filamentous assemblies.
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Cites
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The kinetic mechanism of myosin V
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Mechanism of Blebbistatin Inhibition of Myosin II
Kovács, M., Tóth, J., Hetényi, C., Málnási-Csizmadia, A., & Sellers, J. R. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m405319200 · Cited by 1,019
Self-citationForce Generation, Transmission, and Integration during Cell and Tissue Morphogenesis
Lecuit, T., Lenne, P.-F., & Munro, E. (2011) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100109-104027 · Cited by 621
Specificity of blebbistatin, an inhibitor of myosin II
Limouze, J., Straight, A. F., Mitchison, T., & Sellers, J. R. (2004) · Journal of Muscle Research and Cell Motility · doi:10.1007/s10974-004-6060-7 · Cited by 386
Self-citationMertk drives myosin II redistribution during retinal pigment epithelial phagocytosis
Strick, D. J., Feng, W., & Vollrath, D. (2009) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.08-3058 · Cited by 81
Metadata
- Authors
- Wang, F., Kovács, M., Hu, A., Limouze, J., Harvey, E. V., & Sellers, J. R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-07
- Volume
- 278
- Issue
- 30
- Pages
- 27439-27448
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1966779855
- Cited by
- 253
- References
- 35
- FWCI
- 7.12
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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