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Specificity of blebbistatin, an inhibitor of myosin II
Open accessAbstract
Blebbistatin is a small molecule inhibitor discovered in a screen for inhibitors of nonmuscle myosin IIA. We have examined the specificity and potency of the drug by assaying its effects on the actin-activated MgATPase assay of diverse members of the myosin superfamily. Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. Interestingly, smooth muscle which is highly homologous to vertebrate nonmuscle myosin is only poorly inhibited (IC50=80 μM). The drug potently inhibits Dictyostelium myosin II, but poorly inhibits Acanthamoeba myosin II. Blebbistatin did not inhibit representative myosin superfamily members from classes I, V, and X.
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Cited by
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Mechanism of Blebbistatin Inhibition of Myosin II
Kovács, M., Tóth, J., Hetényi, C., Málnási-Csizmadia, A., & Sellers, J. R. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m405319200 · Cited by 1,019
Self-citationTsai, R. K., & Discher, D. E. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200708043 · Cited by 486
Myosin IIb regulates actin dynamics during synaptic plasticity and memory formation
Rex, C. S., Gavin, C. F., Rubio, M. D., Kramar, E. A., Chen, L. Y., Jia, Y., Huganir, R. L., Muzyczka, N., Gall, C. M., Miller, C. A., et al. (2010) · Neuron · doi:10.1016/j.neuron.2010.07.016 · Cited by 213
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Metadata
- Authors
- Limouze, J., Straight, A. F., Mitchison, T., & Sellers, J. R.
- Venue
- Journal of Muscle Research and Cell Motility
- Published
- 2004-07
- Volume
- 25
- Issue
- 4-5
- Pages
- 337-341
- ISSN
- 0142-4319 (ISSN-L)
- 1573-2657
- OpenAlex
- W2071156256
- Cited by
- 386
- References
- 0
- FWCI
- 5.34
- Type
- journal article
- Language
- en
- Open access
- green
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