The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Sialoside Specificity of the Siglec Family Assessed Using Novel Multivalent Probes
Open accessAbstract
Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their specificity for over 25 synthetic sialosides representing most of the major sequences terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been performed using a novel multivalent platform comprising biotinylated sialosides bound to a streptavidin-alkaline phosphatase conjugate. Each siglec was found to have a unique specificity for binding 16 different sialoside-streptavidin-alkaline phosphatase probes. The relative affinities of monovalent sialosides were assessed for each siglec in competitive inhibition studies. The quantitative data obtained allows a detailed analysis of each siglec for the relative importance of sialic acid and the penultimate oligosaccharide sequence on binding affinity and specificity. Most remarkable was the finding that myelin-associated glycoprotein (Siglec-4) binds with 500-10,000-fold higher affinity to a series of mono- and di-sialylated derivatives of the O-linked T-antigen (Galbeta(1-3)-GalNAc(alpha)OThr) as compared with alpha-methyl-NeuAc.
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Zhang, J. Q., Nicoll, G., Jones, C., & Crocker, P. R. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m002788200 · Cited by 229
Self-citationPaul, S. P., Taylor, L. S., Stansbury, E. K., & McVicar, D. W. (2000) · Blood · doi:10.1182/blood.v96.2.483.014k40_483_490 · Cited by 202
Cited by
Siglec-mediated regulation of immune cell function in disease
Macauley, M. S., Crocker, P. R., & Paulson, J. C. (2014) · Nature Reviews Immunology · doi:10.1038/nri3737 · Cited by 1,122
Self-citationGlycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion
Hudak, J. E., Canham, S. M., & Bertozzi, C. R. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1388 · Cited by 485
Sialic acids in cancer biology and immunity
Pearce, O. M. T., & Läubli, H. (2016) · Glycobiology · doi:10.1093/glycob/cwv097 · Cited by 470
Siglecs as Immune Cell Checkpoints in Disease
Duan, S., & Paulson, J. C. (2020) · Annual Review of Immunology · doi:10.1146/annurev-immunol-102419-035900 · Cited by 452
Self-citationGlycan Array Screening Reveals a Candidate Ligand for Siglec-8*
Bochner, B. S., Alvarez, R. A., Mehta, P., Bovin, N. V., Blixt, O., White, J. R., & Schnaar, R. L. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m412378200 · Cited by 262
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Metadata
- Authors
- Blixt, O., Collins, B. E., van den Nieuwenhof, I. M., Crocker, P. R., & Paulson, J. C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-08
- Volume
- 278
- Issue
- 33
- Pages
- 31007-31019
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1975933816
- Cited by
- 245
- References
- 2
- FWCI
- 4.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Blixt, O., Collins, B. E., van den Nieuwenhof, I. M., Crocker, P. R., & Paulson, J. C. (2003). Sialoside Specificity of the Siglec Family Assessed Using Novel Multivalent Probes. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m304331200
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