The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane
Open accessAbstract
Mitochondria play a crucial role in cellular homeostasis, which justifies the increasing interest in mapping the different components of these organelles. Here we have focused our study on the identification of proteins of the mitochondrial inner membrane (MIM). This membrane is of particular interest because, besides the well known components of the respiratory chain complexes, it contains several ion channels and many carrier proteins that certainly play a key role in mitochondrial function and, therefore, deserve to be identified at the molecular level. To achieve this goal we have used a novel approach combining the use of highly purified mouse liver mitochondrial inner membranes, extraction of membrane proteins with organic acid, and two-dimensional liquid chromatography coupled to tandem mass spectrometry. This procedure allowed us to identify 182 proteins that are involved in several biochemical processes, such as the electron transport machinery, the protein import machinery, protein synthesis, lipid metabolism, and ion or substrate transport. The full range of isoelectric point (3.9-12.5), molecular mass (6-527 kDa), and hydrophobicity values (up to 16 transmembrane predicted domains) were represented. In addition, of the 182 proteins found, 20 were unknown or had never previously been associated with the MIM. Overexpression of some of these proteins in mammalian cells confirmed their mitochondrial localization and resulted in severe remodeling of the mitochondrial network. This study provides the first proteome of the MIM and provides a basis for a more detailed study of the newly characterized proteins of this membrane.
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“Several studies have localized ATAD3A to the mitochondria (143, 144), where is appears to be involved in mitochondrial membrane dynamics.”
From Adrenal Mitochondria and Steroidogenesis: From Individual Proteins to Functional Protein Assemblies (2016) · cites this paper as [144]
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Cites
Perkins, D. N., Pappin, D., Creasy, D. M., & Cottrell, J. S. (1999) · Electrophoresis · doi:10.1002/(sici)1522-2683(19991201)20:18 · Cited by 8,264
Keller, A., Nesvizhskii, A., Kolker, E., & Aebersold, R. (2002) · Analytical Chemistry · doi:10.1021/ac025747h · Cited by 4,970
Large-scale analysis of the yeast proteome by multidimensional protein identification technology
Washburn, M. P., Wolters, D. A., & Yates, J. R. (2001) · Nature Biotechnology · doi:10.1038/85686 · Cited by 4,768
Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
Kamath, R. S., Fraser, A. G., Yan, D., Poulin, G. B., Durbin, R. M., Gotta, M., Kanapin, A. A., Bot, N. L., Moreno, S., Sohrmann, M., et al. (2003) · Nature · doi:10.1038/nature01278 · Cited by 3,629
Cytoprotective Role of Ca 2+ - Activated K + Channels in the Cardiac Inner Mitochondrial Membrane
Xu, W., Liu, Y., Wang, S., McDonald, T. G., Eyk, J. E. V., Sidor, A., & O'Rourke, B. P. (2002) · Science · doi:10.1126/science.1074360 · Cited by 478
Ectopic β-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis
Martinez, L. O., Jacquet, S., Estève, J., Rolland, C., Cabezón, E., Champagne, É., Pineau, T., Georgeaud, V., Walker, J. E., Tercé, F., et al. (2003) · Nature · doi:10.1038/nature01250 · Cited by 459
Analysis of the Arabidopsis Mitochondrial Proteome
Millar, A. H., Sweetlove, L. J., Giegé, P., & Leaver, C. J. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.010387 · Cited by 447
The Mitochondrial K Channel as a Receptor for Potassium Channel Openers
Garlid, K., Pauček, P., Yarov-Yarovoy, V. M., Sun, X., & Schindler, P. A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.15.8796 · Cited by 445
MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation
Goodman, M. B., Ernstrom, G. G., Chelur, D. S., O’Hagan, R., Yao, C. A., & Chalfie, M. (2002) · Nature · doi:10.1038/4151039a · Cited by 321
Mitochondrial intermembrane junctional complexes and their involvement in cell death
Crompton, M., Barksby, E., Johnson, N., & Capano, M. (2002) · Biochimie · doi:10.1016/s0300-9084(02)01368-8 · Cited by 256
Research works printed but not linked: 3
- Taylor, 2003, Nat. Biotechnol., 18, 18
- Anantharaman, 2002, FEMS Microbiol. Lett., 207, 55
- Thorsness, 1993, Mol. Cell. Biol., 13, 5418
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Self-citation
Metadata
- Authors
- Da Cruz, S., Xenarios, I., Langridge, J., Vilbois, F., Parone, P. A., & Martinou, J.-C.
- Affiliations
- University of Geneva (CH)
- Corresponding author
- Martinou, Jean-Claude
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-10
- Volume
- 278
- Issue
- 42
- Pages
- 41566-41571
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2139514626
- Cited by
- 247
- References
- 24
- Citations by year
- 2025: 3 · 2024: 6 · 2023: 3 · 2022: 3 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 7 · 2017: 5 · 2016: 3 · 2015: 12 · 2014: 11 · 2013: 17 · 2012: 15
- FWCI
- 6.65
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- inner mitochondrial membrane, mouse liver mitochondria, mitochondrial proteome, membrane protein identification, respiratory chain complexes, ion channels, carrier proteins, lipid metabolism, mitochondrial network remodeling, mitochondrial localization
- MeSH terms
- Animals, Cations, Cell Membrane, Cell Survival, Chromatography, Liquid, Cloning, Molecular, Female, HeLa Cells, Humans, Immunohistochemistry, Intracellular Membranes, Liver, Mitochondria, Mass Spectrometry, Protein Structure, Tertiary, Proteome, Spectrometry, Mass, Electrospray Ionization, Mice
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