Cited by 889Mitochondrial Function and PathologyBioinformatics and Genomic NetworksAdvanced Proteomics Techniques and Applications

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.

Open access
Mootha, Vamsi K., Bunkenborg, Jakob, Olsen, Jesper V., Hjerrild, Majbrit, Wisniewski, Jacek R., Stahl, Erich, Bolouri, Marjan S., Ray, Heta N. +5 more·2003·Cell

Abstract

Mitochondria are tailored to meet the metabolic and signaling needs of each cell. To explore its molecular composition, we performed a proteomic survey of mitochondria from mouse brain, heart, kidney, and liver and combined the results with existing gene annotations to produce a list of 591 mitochondrial proteins, including 163 proteins not previously associated with this organelle. The protein expression data were largely concordant with large-scale surveys of RNA abundance and both measures indicate tissue-specific differences in organelle composition. RNA expression profiles across tissues revealed networks of mitochondrial genes that share functional and regulatory mechanisms. We also determined a larger "neighborhood" of genes whose expression is closely correlated to the mitochondrial genes. The combined analysis identifies specific genes of biological interest, such as candidates for mtDNA repair enzymes, offers new insights into the biogenesis and ancestry of mammalian mitochondria, and provides a framework for understanding the organelle's contribution to human disease.

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Crossref · 10.1016/s0092-8674(03)00926-7

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Authors
Mootha, V. K., Bunkenborg, J., Olsen, J. V., Hjerrild, M., Wisniewski, J. R., Stahl, E., Bolouri, M. S., Ray, H. N., Sihag, S., Kamal, M., Patterson, N., Lander, E. S., & Mann, M.
Affiliations
  • Brigham and Women's Hospital (US)
  • University of Southern Denmark (DK)
  • Massachusetts Institute of Technology (US)
Corresponding author
  • Lander, Eric S.
  • Mann, Matthias
Venue
Cell
Publisher
Elsevier BV
Published
2003-11
Volume
115
Issue
5
Pages
629-640
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2082516237
Cited by
889
References
51
Citations by year
2026: 6 · 2025: 21 · 2024: 19 · 2023: 28 · 2022: 17 · 2021: 27 · 2020: 32 · 2019: 25 · 2018: 29 · 2017: 31 · 2016: 25 · 2015: 28 · 2014: 32 · 2013: 44 · 2012: 58
FWCI
19.42
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Keywords
mitochondrial proteome, mitochondrial proteins, mitochondrial gene expression, gene regulatory networks, mtDNA repair, mitochondrial biogenesis, brain, heart, kidney, liver
MeSH terms
Animals, Cell Line, DNA, Mitochondrial, Humans, Male, Mitochondria, Oxidative Phosphorylation, RNA, Messenger, Gene Expression Regulation, Enzymologic, DNA Fingerprinting, Cell Respiration, Mitochondrial Proteins, Proteomics, Mice

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