Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Open accessAbstract
Mitochondria are tailored to meet the metabolic and signaling needs of each cell. To explore its molecular composition, we performed a proteomic survey of mitochondria from mouse brain, heart, kidney, and liver and combined the results with existing gene annotations to produce a list of 591 mitochondrial proteins, including 163 proteins not previously associated with this organelle. The protein expression data were largely concordant with large-scale surveys of RNA abundance and both measures indicate tissue-specific differences in organelle composition. RNA expression profiles across tissues revealed networks of mitochondrial genes that share functional and regulatory mechanisms. We also determined a larger "neighborhood" of genes whose expression is closely correlated to the mitochondrial genes. The combined analysis identifies specific genes of biological interest, such as candidates for mtDNA repair enzymes, offers new insights into the biogenesis and ancestry of mammalian mitochondria, and provides a framework for understanding the organelle's contribution to human disease.
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Patterns of gene expression are distinct in liver.
“Similarly, patterns of gene expression are distinct in liver (32).”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [32]
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- Authors
- Mootha, V. K., Bunkenborg, J., Olsen, J. V., Hjerrild, M., Wisniewski, J. R., Stahl, E., Bolouri, M. S., Ray, H. N., Sihag, S., Kamal, M., Patterson, N., Lander, E. S., & Mann, M.
- Affiliations
- Brigham and Women's Hospital (US)
- University of Southern Denmark (DK)
- Massachusetts Institute of Technology (US)
- Corresponding author
- Lander, Eric S.
- Mann, Matthias
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2003-11
- Volume
- 115
- Issue
- 5
- Pages
- 629-640
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2082516237
- Cited by
- 889
- References
- 51
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- FWCI
- 19.42
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- journal article
- Language
- en
- Open access
- bronze
- doi.org
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- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- mitochondrial proteome, mitochondrial proteins, mitochondrial gene expression, gene regulatory networks, mtDNA repair, mitochondrial biogenesis, brain, heart, kidney, liver
- MeSH terms
- Animals, Cell Line, DNA, Mitochondrial, Humans, Male, Mitochondria, Oxidative Phosphorylation, RNA, Messenger, Gene Expression Regulation, Enzymologic, DNA Fingerprinting, Cell Respiration, Mitochondrial Proteins, Proteomics, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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