The DOI matches its Crossref record, with a small difference in title or year; 34 of the 34 references it cites were found in a registry or an index.
Proteomic Analysis of Rat Liver Peroxisome
Open accessAbstract
Subcellular proteomics, which includes isolation of subcellular components prior to a proteomic analysis, is advantageous not only in characterizing large macro-molecular complexes such as organelles but also in elucidating mechanisms of protein transport and organelle biosynthesis. Because of the high sensitivity achieved by the present proteomics technology, the purity of samples to be analyzed is important for the interpretation of the results obtained. In the present study, peroxisomes isolated from rat liver by usual cell fractionation were further purified by immunoisolation using a specific antibody raised against a peroxisomal membrane protein, PMP70. The isolated peroxisomes were analyzed by SDS-PAGE combined with liquid chromatography/mass spectrometry. Altogether 34 known peroxisomal proteins were identified in addition to several mitochondrial and microsomal proteins. Some of the latter may reside in the peroxisomes as well. Analysis of membrane fractions identified all known peroxins except for Pex7. Two new peroxisomal proteins of unknown function were of high abundance. One is a bi-functional protein consisting of an aminoglycoside phosphotransferase-domain and an acyl-CoA dehydrogenase domain. The other is a newly identified peroxisome-specific isoform of Lon protease, an ATP-dependent protease with chaperone-like activity. The peroxisomal localization of the protein was confirmed by immunological techniques. The peroxisome-type Lon protease, which is distinct from the mitochondrial isoform, may play an important role in the peroxisomal biogenesis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Previous proteomic studies using isolated mammalian peroxisomes have identified ACBD5 as a peroxisomal protein (16,17).”
From Deficiency of a Retinal Dystrophy Protein, Acyl-CoA Binding Domain-containing 5 (ACBD5), Impairs Peroxisomal β-Oxidation of Very-long-chain Fatty Acids (2017) · cites this paper as [16]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
Shevchenko, A., Wilm, M. S., Vorm, O., & Mann, M. (1996) · Analytical Chemistry · doi:10.1021/ac950914h · Cited by 8,996
Large-scale analysis of the yeast proteome by multidimensional protein identification technology
Washburn, M. P., Wolters, D. A., & Yates, J. R. (2001) · Nature Biotechnology · doi:10.1038/85686 · Cited by 4,768
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., & Aebersold, R. (1999) · Nature Biotechnology · doi:10.1038/13690 · Cited by 4,683
Proteomics to study genes and genomes
Pandey, A., & Mann, M. (2000) · Nature · doi:10.1038/35015709 · Cited by 2,399
Shevchenko, A., Jensen, O. N., Podtelejnikov, A. V., Sagliocco, F. A., Wilm, M. S., Vorm, O., Mortensen, P. M., Shevchenko, A., Boucherie, H., & Mann, M. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.25.14440 · Cited by 1,429
Lazarow, P. B., & de Duve, C. (1976) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.73.6.2043 · Cited by 1,044
Directed Proteomic Analysis of the Human Nucleolus
Andersen, J., Lyon, C. E., Fox, A. H., Leung, A. K. L., Lam, Y. W., Steen, H., Mann, M., & Lamond, A. I. (2002) · Current Biology · doi:10.1016/s0960-9822(01)00650-9 · Cited by 1,024
Mozdy, A. D., McCaffery, J. M., & Shaw, J. M. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.2.367 · Cited by 731
The application of mass spectrometry to membrane proteomics
Wu, C. C., & Yates, J. R. (2003) · Nature Biotechnology · doi:10.1038/nbt0303-262 · Cited by 561
The mechanism and functions of ATP‐dependent proteases in bacterial and animal cells
Goldberg, A. L. (1992) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1992.tb19822.x · Cited by 446
A Human Gene Responsible for Zellweger Syndrome That Affects Peroxisome Assembly
Shimozawa, N., Tsukamoto, T., Suzuki, Y., Orii, T., Shirayoshi, Y., Mori, T., & Fujiki, Y. (1992) · Science · doi:10.1126/science.1546315 · Cited by 356
Self-citationRequirement for the Yeast Gene LON in Intramitochondrial Proteolysis and Maintenance of Respiration
Suzuki, C. K., Suda, K., Wang, N., & Schatz, G. (1994) · Science · doi:10.1126/science.8146662 · Cited by 272
Konishi, H., Yamauchi, E., Taniguchi, H., Yamamoto, T., Matsuzaki, H., Takemura, Y., Ohmae, K., Kikkawa, U., & Nishizuka, Y. (2001) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.111158798 · Cited by 269
Self-citationProteomics Characterization of Abundant Golgi Membrane Proteins
Bell, A. W., Ward, M. A., Blackstock, W. P., Freeman, H. N., Choudhary, J. S., Lewis, A. P., Chotai, D., Fazel, A., Gushue, J. N., Paiement, J. M., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m006143200 · Cited by 246
van Dyck, L., Pearce, D. A., & Sherman, F. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42340-4 · Cited by 233
Chloroplast and Mitochondrial Proteases in Arabidopsis. A Proposed Nomenclature
Adam, Z., Adamska, I., Nakabayashi, K., Ostersetzer, O., Haußühl, K., Manuell, A. L., Zheng, B., Vallon, O., Rodermel, S. R., Shinozaki, K., et al. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.125.4.1912 · Cited by 216
A human mitochondrial ATP-dependent protease that is highly homologous to bacterial Lon protease.
Wang, N., Gottesman, S., Willingham, M. C., Gottesman, M. M., & Maurizi, M. R. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.23.11247 · Cited by 186
[25] ATP-dependent protease La (Lon) from Escherichia coli
Goldberg, A. L., Moerschell, R. P., Hachung, C., & Maurizi, M. R. (1994) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(94)44027-1 · Cited by 178
Insertion of the 70-kDa Peroxisomal Membrane Protein into Peroxisomal Membranes in Vivo and in Vitro
Imanaka, T., Shiina, Y., Takano, T., Hashimoto, T., & Osumi, T. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.7.3706 · Cited by 123
Self-citationSarria, R., Lyznik, A., Vallejos, C. E., & Mackenzie, S. A. (1998) · The Plant Cell · doi:10.1105/tpc.10.7.1217 · Cited by 113
Suzuki, Y., Jiang, L., Souri, M., Miyazawa, S., Fukuda, S., Zhang, Z., Une, M., Shimozawa, N., Kondo, N., Orii, T., et al. (1997) · The American Journal of Human Genetics · doi:10.1086/301599 · Cited by 107
Self-citationDecker, G., Wanner, G., Zenk, M. H., & Lottspeich, F. (2000) · Electrophoresis · doi:10.1002/1522-2683(20001001)21:16 · Cited by 102
Helledie, T., Grøntved, L., Jensen, S. S., Kiilerich, P., RIETVELD, L. E. G., Albrektsen, T., Sandberg, M. B., Nøhr, J., Larsen, L. K., Fleckner, J., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m111295200 · Cited by 99
The human proteomics initiative (HPI)
O’Donovan, C., Apweiler, R., & Bairoch, A. (2001) · Trends in biotechnology · doi:10.1016/s0167-7799(01)01598-0 · Cited by 86
Fowler, S., Remacle, J., Trouet, A., Beaufay, H., Berthet, J., Wibo, M., & Hauser, P. (1976) · The Journal of Cell Biology · doi:10.1083/jcb.71.2.535 · Cited by 63
Miyauchi, K., Masaki, R., Taketani, S., Yamamoto, A., Akayama, M., & Tashiro, Y. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55028-6 · Cited by 62
Yoshihara, T., Hamamoto, T., Munakata, R., Tajiri, R., Ohsumi, M., & Yokota, S. (2001) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540104900906 · Cited by 58
Self-citationMohan, K. V. K., Som, I., & Atreya, C. D. (2002) · Journal of Virology · doi:10.1128/jvi.76.5.2543-2547.2002 · Cited by 51
A mass spectrometric study on the in vivo posttranslational modification of GAP-43.
Taniguchi, H., Suzuki, M., Manenti, S., & Titani, K. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)31670-8 · Cited by 50
Self-citationMaize contains a Lon protease gene that can partially complement a yeast pim1-deletion mutant
Barakat, S., Pearce, D. A., Sherman, F., & Rapp, W. D. (1998) · Plant Molecular Biology · doi:10.1023/a:1005912831051 · Cited by 37
Shimozawa, N., Nagase, T., Takemoto, Y., Ohura, T., Suzuki, Y., & Kondo, N. (2003) · American Journal of Medical Genetics Part A · doi:10.1002/ajmg.a.20030 · Cited by 34
Self-citationWalker, A. K., Rymar, G., & Andrews, P. (2001) · Electrophoresis · doi:10.1002/1522-2683()22:5 · Cited by 29
Purification and Characterization of MAR1
Alfonzo, J. D., Thiemann, O. H., & Simpson, L. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.45.30003 · Cited by 22
A novel 57 kDa peroxisomal membrane polypeptide detected by monoclonal antibody (PXM1a/207B)
Imanaka, T., Lazarow, P. B., & Takano, T. (1991) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(91)90401-s · Cited by 21
Self-citation
Research works printed but not linked: 1
- Sarria, 1998, Plant Cell., 10, 1217
Cited by
Sorted by citations
MOLECULAR AND EVOLUTIONARY BASIS OF THE CELLULAR STRESS RESPONSE
Kültz, D. (2004) · Annual Review of Physiology · doi:10.1146/annurev.physiol.67.040403.103635 · Cited by 1,556
Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae
Galagan, J. E., Calvo, S. E., Cuomo, C. A., Ma, L., Wortman, J. R., Batzoglou, S., Lee, S., Baştürkmen, M., Spevak, C. C., Clutterbuck, J., et al. (2005) · Nature · doi:10.1038/nature04341 · Cited by 1,359
Role of peroxisomes in ros/rns-metabolism: Implications for human disease
Fransen, M., Nordgren, M., Wang, B., & Apanasets, O. (2012) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2011.12.001 · Cited by 644
PGC7/Stella protects against DNA demethylation in early embryogenesis
Nakamura, T., Arai, Y., Umehara, H., Masuhara, M., Kimura, T., Taniguchi, H., Sekimoto, T., Ikawa, M., Yoneda, Y., Okabe, M., et al. (2006) · Nature Cell Biology · doi:10.1038/ncb1519 · Cited by 610
Self-citationContribution of the Endoplasmic Reticulum to Peroxisome Formation
Hoepfner, D., Schildknegt, D., Braakman, I., Philippsen, P., & Tabak, H. F. (2005) · Cell · doi:10.1016/j.cell.2005.04.025 · Cited by 463
Metadata
- Authors
- Kikuchi, M., Hatano, N., Yokota, S., Shimozawa, N., Imanaka, T., & Taniguchi, H.
- Affiliations
- SPring-8 (JP)
- University of Yamanashi (JP)
- Gifu University (JP)
- University of Toyama (JP)
- Corresponding author
- Taniguchi, Hisaaki
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-01
- Volume
- 279
- Issue
- 1
- Pages
- 421-428
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2063731065
- Cited by
- 258
- References
- 34
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 6 · 2023: 9 · 2022: 3 · 2021: 3 · 2020: 8 · 2019: 6 · 2018: 9 · 2017: 6 · 2016: 11 · 2015: 11 · 2014: 11 · 2013: 13 · 2012: 12
- FWCI
- 7.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Metabolism and Genetic Disorders
- Keywords
- peroxisomes, peroxisomal proteins, peroxisome biogenesis, PMP70, immunoisolation, subcellular proteomics, peroxins, Lon protease, protein localization, rat liver
- MeSH terms
- Animals, Cell Fractionation, Enzymes, Heat-Shock Proteins, Isoenzymes, Liver, Regression Analysis, Serine Endopeptidases, Microscopy, Immunoelectron, Proteome, Peroxisomes, Protein Transport, ATP-Dependent Proteases, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
