Cited by 103Cellular transport and secretionReceptor Mechanisms and SignalingProtein Kinase Regulation and GTPase Signaling

Distinct Pathways for the Trafficking of Angiotensin II and Adrenergic Receptors from the Endoplasmic Reticulum to the Cell Surface

Open access
Wu, Guangyu, Zhao, Guiqing, He, Youe·2003·Journal of Biological Chemistry

Abstract

The molecular mechanism underlying the transport of G protein-coupled receptors from the endoplasmic reticulum (ER) to the cell surface is poorly understood. This issue was addressed by determining the role of Rab1, a Ras-related small GTPase that coordinates vesicular protein transport in the early secretory pathway, in the subcellular distribution and function of the angiotensin II type 1A receptor (AT1R), beta2-adrenergic receptor (AR), and alpha2B-AR in HEK293T cells. Inhibition of endogenous Rab1 function by transient expression of dominant-negative Rab1 mutants or Rab1 small interfering RNA (siRNA) induced a marked perinuclear accumulation and a significant reduction in cell-surface expression of AT1R and beta2-AR. The accumulated receptors were colocalized with calregulin (an ER marker) and GM130 (a Golgi marker), consistent with Rab1 function in regulating protein transport from the ER to the Golgi. In contrast, dominant-negative Rab1 mutants and siRNA had no effect on the subcellular distribution of alpha2B-AR. Similarly, expression of dominant-negative Rab1 mutants and siRNA depletion of Rab1 significantly attenuated AT1R-mediated inositol phosphate accumulation and ERK1/2 activation and beta2-AR-mediated ERK1/2 activation, but not alpha2B-AR-stimulated ERK1/2 activation. These data indicate that Rab1 GTPase selectively regulates intracellular trafficking and signaling of G protein-coupled receptors and suggest a novel, as yet undefined pathway for movement of G pro…

Integrity signals

Registry

Crossref · 10.1074/jbc.m305707200

Cites

53 of 53 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Wu, G., Zhao, G., & He, Y.
Affiliations
  • Louisiana State University (US)
  • Louisiana State University Health Sciences Center New Orleans (US)
Corresponding author
Wu, Guangyu
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2003-11
Volume
278
Issue
47
Pages
47062-47069
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2059443787
Cited by
103
References
53
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 6 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 9 · 2014: 2 · 2013: 4 · 2012: 6
FWCI
2.31
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
Rab1, G protein-coupled receptor trafficking, endoplasmic reticulum to Golgi transport, angiotensin II type 1a receptor, beta2-adrenergic receptor, alpha2B-adrenergic receptor, ERK1/2 activation, inositol phosphate accumulation, receptor subcellular distribution, HEK293T cells
MeSH terms
Animals, Cell Line, Cell Membrane, Endoplasmic Reticulum, Golgi Apparatus, Humans, Inositol Phosphates, Receptors, Adrenergic, Transfection, Receptors, Adrenergic, alpha-2, Receptors, Adrenergic, beta-2, rab1 GTP-Binding Proteins, Mitogen-Activated Protein Kinases, Protein Transport, Receptors, G-Protein-Coupled, Receptor, Angiotensin, Type 1, Mitogen-Activated Protein Kinase 3, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review