The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Mitochondrial pH Monitored by a New Engineered Green Fluorescent Protein Mutant
Open accessAbstract
We here describe a new molecularly engineered green fluorescent protein chimera that shows a high sensitivity to pH in the alkaline range. This probe was named mtAlpHi, for mitochondrial alkaline pH indicator, and possesses several key properties that render it optimal for studying the dynamics of mitochondrial matrix pH, e.g. it has an apparent pK(a) (pK(a)') around 8.5, it shows reversible and large changes in fluorescence in response to changes in pH (both in vitro and in intact cells), and it is selectively targeted to the mitochondrial matrix. Using mtAlpHi we could monitor pH changes that occur in the mitochondrial matrix in a variety of situations, e.g. treatment with uncouplers or Ca(2+) ionophores, addition of drugs that interfere with ATP synthesis or electron flow in the respiratory chain, weak bases or acids, and receptor activation. We observed heterogeneous pH increases in the mitochondrial matrix during Ca(2+) accumulation by this organelle. Finally, we demonstrate that Ca(2+) mobilization from internal stores induced by ionomycin and A23187 cause a dramatic acidification of the mitochondrial matrix.
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Cites
Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
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Self-citationMitochondrial Transport of Cations: Channels, Exchangers, and Permeability Transition
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Mitochondria and neuronal survival
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Self-citation
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Sensors and regulators of intracellular pH
Casey, J. R., Grinstein, S., & Orlowski, J. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2820 · Cited by 2,218
Mitochondria are physiologically maintained at close to 50 °C
Chrétien, D., Bénit, P., Ha, H.-H., Keipert, S., El-Khoury, R., Chang, Y.-T., Jastroch, M., Jacobs, H. T., Rustin, P., & Rak, M. (2018) · PLOS Biology · doi:10.1371/journal.pbio.2003992 · Cited by 448
Mitochondrial calcium exchange in physiology and disease
Garbincius, J. F., & Elrod, J. W. (2022) · Physiological Reviews · doi:10.1152/physrev.00041.2020 · Cited by 412
Intracellular pH is a tightly controlled signal in yeast
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Metadata
- Authors
- Abad, M. F. C., Di Benedetto, G., Magalhães, P. J., Filippin, L., & Pozzan, T.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-03
- Volume
- 279
- Issue
- 12
- Pages
- 11521-11529
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1963957340
- Cited by
- 258
- References
- 29
- FWCI
- 4.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Abad, M. F. C., Di Benedetto, G., Magalhães, P. J., Filippin, L., & Pozzan, T. (2004). Mitochondrial pH Monitored by a New Engineered Green Fluorescent Protein Mutant. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m306766200
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