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A Fatty Acid Synthase Blockade Induces Tumor Cell-cycle Arrest by Down-regulating Skp2
Open accessAbstract
In eukaryotes, fatty acid synthase (FAS) is the enzyme responsible for synthesis of palmitate, the precursor of long-chain nonessential fatty acids. FAS is up-regulated in a wide range of cancers and has been suggested as a relevant drug target. Here, two independent approaches are taken toward knocking down FAS and then probing its connection to tumor cell proliferation. In one approach, Orlistat, a drug approved for treating obesity, is used as a potent inhibitor of the thioesterase function of FAS. In a separate strategy, the expression of FAS is suppressed by targeted knock-down with small interfering RNA. In both circumstances, the ablation of FAS activity causes a dramatic down-regulation of Skp2, a component of the E3 ubiquitin ligase that controls the turnover of p27Kip1. These effects ultimately tie into the retinoblastoma protein pathway and lead to a cell-cycle arrest at the G1/S boundary. Altogether, the findings of the study reveal unappreciated links between fatty acid synthase and ubiquitin-dependent proteolysis of cell-cycle regulatory proteins.
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“FASN can promote cancer progression and contribute to therapy resistance by sustaining mitogenic signaling, facilitating the G1/S transition, and supplying lipid resources necessary for biomass accumulation and evasion of cell cycle checkpoints [30,48].”
From Inhibition of fatty acid synthase enhances therapeutic efficacy and delays acquired resistance to BRAF-targeted therapy in colorectal cancer (2026) · cites this paper as [48]
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Metadata
- Authors
- Knowles, L. M., Axelrod, F., Browne, C. D., & Smith, J. W.
- Affiliations
- Sanford Burnham Prebys Medical Discovery Institute (US)
- Corresponding author
- Smith, Jeffrey W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-07
- Volume
- 279
- Issue
- 29
- Pages
- 30540-30545
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2115307821
- Cited by
- 146
- References
- 38
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 6 · 2023: 5 · 2022: 2 · 2021: 4 · 2019: 4 · 2018: 5 · 2017: 7 · 2016: 4 · 2015: 5 · 2014: 12 · 2013: 6 · 2012: 13
- FWCI
- 3.48
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA30199, CA-69306, R01CA069306, CA6982713, P30CA030199)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Keywords
- fatty acid synthase, Skp2, orlistat, cell cycle arrest, G1/S checkpoint, p27, ubiquitin-mediated proteolysis, E3 ubiquitin ligase, tumor cell proliferation, siRNA knockdown, cancer drug targets
- MeSH terms
- Orlistat, Avidin, Biotin, Cell Cycle, Cell Division, Cell Line, Dose-Response Relationship, Drug, Epithelial Cells, Fatty Acids, Humans, Lactones, Lipase, Obesity, Serine Endopeptidases, Time Factors, Blotting, Western, Down-Regulation, Retinoblastoma Protein, G1 Phase, S Phase, Cell Cycle Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Palmitic Acid, Tumor Suppressor Proteins, Ubiquitin, RNA, Small Interfering, Ubiquitin-Protein Ligases, S-Phase Kinase-Associated Proteins, Cell Line, Tumor, Cyclin-Dependent Kinase Inhibitor p27, Fatty Acid Synthases
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