The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Prostaglandin I2 Production and cAMP Accumulation in Response to Acidic Extracellular pH through OGR1 in Human Aortic Smooth Muscle Cells
Open accessAbstract
Ovarian cancer G-protein-coupled receptor 1 (OGR1) and GPR4 have recently been identified as proton-sensing or extracellular pH-responsive G-protein-coupled receptors stimulating inositol phosphate production and cAMP accumulation, respectively. In the present study, we found that OGR1 and GPR4 mRNAs were expressed in human aortic smooth muscle cells (AoSMCs). Acidic extracellular pH induced inositol phosphate production, a transient increase in intracellular Ca(2+) concentration ([Ca(2+)](i)), and cAMP accumulation in these cells. When small interfering RNAs (siRNAs) targeted for OGR1 and GPR4 were transfected to the cells, the acid-induced inositol phosphate production and [Ca(2+)](i) increase were markedly inhibited by the OGR1 siRNA but not by the GPR4 siRNA. Unexpectedly, the acid-induced cAMP accumulation was also largely inhibited by OGR1 siRNA but only slightly by GPR4 siRNA. Acidic extracellular pH also stimulated prostaglandin I2 (PGI(2)) production, which was again inhibited by OGR1 siRNA. The specific inhibitors for extracellular signal-regulated kinase kinase and cyclooxygenase attenuated the acid-induced PGI(2) production and cAMP accumulation without changes in the inositol phosphate production. A specific inhibitor of phospholipase C also inhibited the acid-induced cAMP accumulation. In conclusion, OGR1 is a major receptor involved in the extracellular acid-induced stimulation of PGI(2) production and cAMP accumulation in AoSMCs. The cAMP accumulation may occur through OGR1-mediated stimulation of the phospholipase C/cyclooxygenase/PGI(2) pathway.
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“Medium acidification during in vitro cell culture can also initiate pro-inflammatory signaling responses in human aortic smooth muscle cells (Tomura et al., 2005) and cells of the human nucleus pulposus (Gilbert et al., 2016).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Tomura et al., 2005
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Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1
Xu, Y., Zhu, K., Hong, G., Wu, W., Baudhuin, L. M., Xiao, Y.-J., & Damron, D. S. (2000) · Nature Cell Biology · doi:10.1038/35010529 · Cited by 289
RetractedZhu, K., Baudhuin, L. M., Hong, G., Williams, F. S., Cristina, K. L., Kabarowski, J. H. S., Witte, O. N., & Xu, Y. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m008057200 · Cited by 242
RetractedThe Immune Modulator FTY720 Targets Sphingosine 1-Phosphate Receptors
Brinkmann, V., Davis, M. D., Heise, C. E., Albert, R., Cottens, S., Hof, R., Bruns, C., Prieschl, E. E., Baumruker, T., HIESTAND, P. C., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200176200 · Cited by 1,512
Proton-sensing G-protein-coupled receptors
Ludwig, M., Vanek, M., Guerini, D., Gasser, J. A., Jones, C. E., JUNKER, U., Hofstetter, H., Wolf, R. M., & Seuwen, K. (2003) · Nature · doi:10.1038/nature01905 · Cited by 746
Therapeutic effects of lysophosphatidylcholine in experimental sepsis
Yan, J.-J., JUNG, J., Lee, J.-E., Lee, J.-H., Huh, S., Kim, H., Jung, K. C., Cho, J., Nam, J., Suh, H., et al. (2004) · Nature Medicine · doi:10.1038/nm989 · Cited by 328
TDAG8 Is a Proton-sensing and Psychosine-sensitive G-protein-coupled Receptor
Wang, J.-Q., Kon, J., Mogi, C., Tobo, M., Damirin, A., Sato, K., Komachi, M., Malchinkhuu, E., Murata, N., Kimura, T., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m406966200 · Cited by 256
Self-citationG2A Is a Proton-sensing G-protein-coupled Receptor Antagonized by Lysophosphatidylcholine
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Okajima, F. (2002) · Biochimica et Biophysica Acta · doi:10.1016/s1388-1981(02)00147-6 · Cited by 244
Self-citationMice Lacking the Orphan G Protein-Coupled Receptor G2A Develop a Late-Onset Autoimmune Syndrome
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Radu, C. G., Nijagal, A., McLaughlin, J., Wang, L., & Witte, O. N. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409415102 · Cited by 213
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- Leffler, 1999, Am. J. Physiol., 277, H1878
- Sato, 1999, Mol. Pharmacol., 55, 126, 10.1124/mol.55.1.126 · DOI
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Thornell, I. M., Michenkova, M., Taki, S., Bevensee, M. O., & Boron, W. F. (2026) · Elsevier eBooks · doi:10.1016/b978-0-12-815389-5.00077-0
Genetic and epigenetic modifiers of metastasis: Context matters
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Metadata
- Authors
- Tomura, H., Wang, J.-Q., Komachi, M., Damirin, A., Mogi, C., Tobo, M., Kon, J., Misawa, N., Sato, K., & Okajima, F.
- Affiliations
- Gunma University (JP)
- Kirin (Japan) (JP)
- Corresponding author
- Tomura, Hideaki
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2005-10
- Volume
- 280
- Issue
- 41
- Pages
- 34458-34464
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2076643673
- Cited by
- 103
- References
- 42
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 2 · 2023: 2 · 2022: 8 · 2021: 7 · 2020: 5 · 2019: 8 · 2018: 2 · 2017: 2 · 2016: 8 · 2015: 10 · 2014: 3 · 2013: 6 · 2012: 3
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Nakatomi Foundation
- Uehara Memorial Foundation
- Japan Society for the Promotion of Science
- Topics
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Keywords
- OGR1, GPR4, proton-sensing G protein-coupled receptor, extracellular acidosis, prostacyclin, cAMP accumulation, inositol phosphate formation, intracellular calcium, aortic smooth muscle cells, phospholipase C, cyclooxygenase, ERK kinase
- MeSH terms
- Cyclic AMP, Aorta, Calcium, Enzyme Inhibitors, Humans, Hydrogen-Ion Concentration, Inositol Phosphates, Type C Phospholipases, Epoprostenol, Transfection, Signal Transduction, Reverse Transcriptase Polymerase Chain Reaction, Myocytes, Smooth Muscle, RNA, Small Interfering, Receptors, G-Protein-Coupled
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