A Sensitive Flow Cytometry-based Nucleotide Excision Repair Assay Unexpectedly Reveals That Mitogen-activated Protein Kinase Signaling Does Not Regulate the Removal of UV-induced DNA Damage in Human Cells
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In response to diverse genotoxic stimuli (e.g. UV and cisplatin), the mitogen-activated protein kinases ERK1/2, JNK1/2, and p38alpha/beta become rapidly phosphorylated and in turn activate multiple downstream effectors that modulate apoptosis and/or growth arrest. Furthermore, previous lines of evidence have strongly suggested that ERK1/2 and JNK1/2 participate in global-genomic nucleotide excision repair, a critical antineoplastic pathway that removes helix-distorting DNA adducts induced by a variety of mutagenic agents, including UV. To rigorously evaluate the potential role of mitogen-activated protein kinases in global-genomic nucleotide excision repair, various human cell strains (primary skin fibroblasts, primary lung fibroblasts, and HCT116 colon carcinoma cells) were treated with highly specific chemical inhibitors, which, following UV exposure, (i) abrogated the capacities of ERK1/2, JNK1/2, or p38alpha/beta to phosphorylate specific downstream effectors and (ii) characteristically modulated cellular proliferation, clonogenic survival, and/or apoptosis. A highly sensitive flow cytometry-based nucleotide excision repair assay recently optimized and validated in our laboratory was then employed to directly demonstrate that the kinetics of UV DNA photoadduct repair are highly similar in mock-treated versus mitogen-activated protein kinase inhibitor-treated cells. These data on primary and tumor cells treated with pharmacological inhibitors were fully corroborated by repair studies using (i) short hairpin RNA-mediated knockdown of ERK1/2 or JNK1/2 in human U2OS osteosarcoma cells and (ii) expression of a dominant negative p38alpha mutant in human primary lung fibroblasts. Our results provide solid evidence for the first time, in disaccord with a burgeoning perception, that mitogen-activated protein kinase signaling does not influence the efficiency of human global-genomic nucleotide excision repair.
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Nucleotide excision repair of 6-4 PPs is generally much more efficient than repair of CPDs.
“The nucleotide excision repair of (6–4) PPs is generally much more efficient than the repair of CPDs 78, 79”
From Xeroderma Pigmentosa Group A (XPA), Nucleotide Excision Repair and Regulation by ATR in Response to Ultraviolet Irradiation (2017) · cites this paper as [78]
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Cites
A protein kinase involved in the regulation of inflammatory cytokine biosynthesis
Lee, J. C., Laydon, J. T., McDonnell, P., Gallagher, T. F., Kumar, S., Green, D., McNulty, D. E., Blumenthal, M. J., Keys, J., vatter, S. W. L., et al. (1994) · Nature · doi:10.1038/372739a0 · Cited by 3,266 · see citing works
Identification of a Novel Inhibitor of Mitogen-activated Protein Kinase Kinase
Favata, M. F., Horiuchi, K. Y., Manos, E. J., Daulerio, A. J., Stradley, D. A., Feeser, W. S., Dyk, D. E. V., Pitts, W. J., Earl, R. A., Hobbs, F. W., et al. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.29.18623 · Cited by 3,016 · see citing works
SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase
Bennett, B. L., Sasaki, D. T., Murray, B. W., O'Leary, E. C., Sakata, S. T., Xu, W., Leisten, J. C., Motiwala, A., Pierce, S., Satoh, Y., et al. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.251194298 · Cited by 2,558 · see citing works
Raingeaud, J., Gupta, S., Rogers, J. S., Dickens, M., Han, J., Ulevitch, R. J., & Davis, R. J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.13.7420 · Cited by 2,244 · see citing works
DNA Repair by ERCC1 in Non–Small-Cell Lung Cancer and Cisplatin-Based Adjuvant Chemotherapy
Olaussen, K. A., Dunant, A., Fouret, P. J., Brambilla, E. M., André, F., Haddad, V., Clermont, E., Filipits, M., Pirker, R., Popper, H., et al. (2006) · New England Journal of Medicine · doi:10.1056/nejmoa060570 · Cited by 1,725 · see citing works
Targeting the mitogen-activated protein kinase cascade to treat cancer
Sebolt, J. S., & Herrera, R. (2004) · Nature reviews. Cancer · doi:10.1038/nrc1503 · Cited by 1,103 · see citing works
Mammalian DNA nucleotide excision repair reconstituted with purified protein components
Aboussekhra, A., Biggerstaff, M., Shivji, M. K. K., Vilpo, J. A., Moncollin, V., Podust, V. N., Protić, M., Hübscher, U., Egly, J.-M., & Wood, R. D. (1995) · Cell · doi:10.1016/0092-8674(95)90289-9 · Cited by 837 · see citing works
Role of JNK activation in apoptosis: A double-edged sword
Liu, J., & Lin, A. (2005) · Cell Research · doi:10.1038/sj.cr.7290262 · Cited by 736 · see citing works
Bulavin, D. V., Saito, S., Hollander, M. C., Sakaguchi, K., Anderson, C. W., Appella, E., & Fornace, A. J. (1999) · The EMBO Journal · doi:10.1093/emboj/18.23.6845 · Cited by 704 · see citing works
Hwang, B. J., Ford, J. M., Hanawalt, P. C., & Chu, G. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.2.424 · Cited by 581 · see citing works
The c-jun kinase/stress-activated pathway: Regulation, function and role in human disease
Johnson, G. L., & Nakamura, K. (2007) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2006.12.009 · Cited by 505 · see citing works
Kraemer, K. H., Patronas, N. J., Schiffmann, R., Brooks, B. P., Tamura, D., & DiGiovanna, J. J. (2007) · Neuroscience · doi:10.1016/j.neuroscience.2006.12.020 · Cited by 443 · see citing works
Smith, M. L., Ford, J. M., Hollander, M. C., Bortnick, R. A., Amundson, S. A., Seo, Y. R., Deng, C., Hanawalt, P. C., & Fornace, A. J. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.10.3705-3714.2000 · Cited by 432 · see citing works
Cancer in xeroderma pigmentosum and related disorders of DNA repair
Cleaver, J. E. (2005) · Nature Reviews Cancer · doi:10.1038/nrc1652 · Cited by 427 · see citing works
Manke, I. A., Nguyen, A., Lim, D., Stewart, M. Q., Elia, A. E. H., & Yaffe, M. B. (2005) · Molecular Cell · doi:10.1016/j.molcel.2004.11.021 · Cited by 416 · see citing works
Cellular and molecular events leading to the development of skin cancer
Melnikova, V. O., & Ananthaswamy, H. N. (2005) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2004.11.015 · Cited by 370 · see citing works
ERKs and p38 Kinase Phosphorylate p53 Protein at Serine 15 in Response to UV Radiation
She, Q., Chen, N., & Dong, Z. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m001020200 · Cited by 345 · see citing works
Ford, J. M., & Hanawalt, P. C. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.19.8876 · Cited by 343 · see citing works
p53 and DNA damage-inducible expression of the xeroderma pigmentosum group C gene
Adimoolam, S., & Ford, J. M. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.202485699 · Cited by 321 · see citing works
Buschmann, T., Potapova, O., Bar‐Shira, A., Ivanov, V. N., Fuchs, S. Y., Henderson, S. C., Fried, V. A., Minamoto, T., Alarcon-Vargas, D., Pincus, M. R., et al. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.8.2743-2754.2001 · Cited by 292 · see citing works
Mitchell, D. L., Haipek, C. A., & Clarkson, J. M. (1985) · Mutation Research Letters · doi:10.1016/s0165-7992(85)80018-x · Cited by 277 · see citing works
p38 Kinase Mediates UV-induced Phosphorylation of p53 Protein at Serine 389
Huang, C., Ma, W.-Y., Maxiner, A., Sun, Y., & Dong, Z. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.18.12229 · Cited by 274 · see citing works
p53 is a chromatin accessibility factor for nucleotide excision repair of DNA damage
Rubbi, C. P., & Milner, J. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg082 · Cited by 229 · see citing works
Potapova, O., Haghighi, A. P., Bost, F., Liu, C., Birrer, M. J., Gjerset, R. A., & Mercola, D. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.22.14041 · Cited by 229 · see citing works
Nojima, K., Hochegger, H., Saberi, A., Fukushima, T., Kikuchi, K., Yoshimura, M., Orelli, B. J., Bishop, D. K., Hirano, S., Ohzeki, M., et al. (2005) · Cancer Research · doi:10.1158/0008-5472.can-05-1214 · Cited by 185 · see citing works
Hayakawa, J., Depatie, C., Ohmichi, M., & Mercola, D. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m210992200 · Cited by 158 · see citing works
c-Jun N-terminal kinase (JNK) is required for survival and proliferation of B-lymphoma cells
Gururajan, M., Chui, R., Karuppannan, A. K., Ke, J., JENNINGS, C. D., & Bondada, S. (2005) · Blood · doi:10.1182/blood-2004-10-3819 · Cited by 156 · see citing works
Du, L., Lyle, C. S., Obey, T. B., Gaarde, W. A., Muir, J. A., Bennett, B. L., & Chambers, T. C. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m304935200 · Cited by 144 · see citing works
Clifton, A. D., Young, P. R., & Cohen, P. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00816-2 · Cited by 141 · see citing works
Protective Role for c-Jun in the Cellular Response to DNA Damage
Potapova, O., Basu, S., Mercola, D., & Holbrook, N. J. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102075200 · Cited by 116 · see citing works
MEK1 and MEK2, Different Regulators of the G1/S Transition
Ussar, S., & Voss, T. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m406240200 · Cited by 115 · see citing works
Therrien, J., Drouin, R., Baril, C., & Drobetsky, E. A. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.26.15038 · Cited by 92 · see citing works
Self-citationp38 MAPK in regulating cellular responses to ultraviolet radiation
Li, J., Yingbin, Z., & Wang, C. (2007) · Journal of Biomedical Science · doi:10.1007/s11373-007-9148-4 · Cited by 85 · see citing works
Revisiting the rodent repairadox
Hanawalt, P. C. (2001) · Environmental and Molecular Mutagenesis · doi:10.1002/em.1057 · Cited by 82 · see citing works
Role of MAP kinases in UVB-induced phosphorylation of p53 at serine 20
She, Q., Ma, W., & Dong, Z. (2002) · Oncogene · doi:10.1038/sj.onc.1205239 · Cited by 73 · see citing works
Andrieux, L. O., Fautrel, A., Bessard, A., Guillouzo, A., Baffet, G., & Langouët, S. (2007) · Cancer Research · doi:10.1158/0008-5472.can-06-3821 · Cited by 68 · see citing works
Mathonnet, G., Léger, C., Desnoyers, J., Drouin, R., Therrien, J., & Drobetsky, E. A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1232161100 · Cited by 56 · see citing works
Self-citationChristmann, M., Tomicic, M. T., Aasland, D., & Kaina, B. (2006) · Carcinogenesis · doi:10.1093/carcin/bgl119 · Cited by 51 · see citing works
Christmann, M., Tomicic, M. T., Origer, J., Aasland, D., & Kaina, B. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl895 · Cited by 48 · see citing works
Chromosome analysis of lymphocytes from workers at an ethylene oxide plant
Clare, M. G., Dean, B. J., de Jong, G., & van Sittert, N. J. (1985) · Mutation Research/Genetic Toxicology · doi:10.1016/0165-1218(85)90013-8 · Cited by 32 · see citing works
Hildesheim, J., & Fornace, A. J. (2004) · DNA repair · doi:10.1016/j.dnarep.2004.02.012 · Cited by 32 · see citing works
Gjerset, R. A., Lebedeva, S., Haghighi, A. P., Turla, S. T., & Mercola, D. (1999) · PubMed · Cited by 30 · see citing works
Berg, R. J. W., de Gruijl, F. R., Roza, L., & van der Leun, J. C. (1993) · Carcinogenesis · doi:10.1093/carcin/14.1.103 · Cited by 24 · see citing works
Loignon, M. (2002) · Carcinogenesis · doi:10.1093/carcin/23.1.35 · Cited by 17 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 6
- Mathonnet, G., Leger, C., Desnoyers, J., Drouin, R., Therrien, J. P., and Drobetsky, E. A. (2003) Proc. Natl. Acad. Sci. 100, 7219 -7224
- Ford, J. M., and Hanawalt, P. C. (1995) Proc. Natl. Acad. Sci. 92, 8876 -8880
- Adimoolam, S., and Ford, J. M. (2002) Proc. Natl. Acad. Sci. 99, 12985-12990
- Hwang, B. J., Ford, J. M., Hanawalt, P. C., and Chu, G. (1999) Proc. Natl. Acad. Sci. 96, 424 -428
- Therrien, J. P., Drouin, R., Baril, C., and Drobetsky, E. A. (1999) Proc. Natl. Acad. Sci. 96, 15038 -15043
- Bennett, B. L., Sasaki, D. T., Murray, B. W., O'Leary, E. C., Sakata, S. T., Xu, W., Leisten, J. C., Motiwala, A., Pierce, S., Satoh, Y., Bhagwat, S. S., Manning, A. M., and Anderson, D. W. (2001) Proc. Natl. Acad. Sci. 98, 13681-13686
Cited by
Sorted by citations
Auclair, Y., Rouget, R., Affar, E. B., & Drobetsky, E. A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0801585105 · Cited by 91 · see citing works
Self-citationThe 6-4 Photoproduct Is the Trigger of UV-Induced Replication Blockage and ATR Activation
Hung, K.-F., Sidorova, J. M., Nghiem, P., & Kawasumi, M. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1917196117 · Cited by 64 · see citing works
Cai, Q., Fu, L., Wang, Z., Gan, N., Dai, X., & Wang, Y. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.558510 · Cited by 57 · see citing works
Bélanger, F., Fortier, E., Dubé, M., Lemay, J.-F., Buisson, R., Masson, J.-Y., Elsherbiny, A., Costantino, S., Carmona, E., Mes-Masson, A.-M., et al. (2018) · Cancer Research · doi:10.1158/0008-5472.can-18-0618 · Cited by 58 · see citing works
Self-citationZhao, Q., Barakat, B. M., Qin, S., Ray, A., El‐Mahdy, M. A., Wani, G., Arafa, E. A., Mir, S. N., Wang, Q., & Wani, A. A. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m803963200 · Cited by 54 · see citing works
Goto, N., Bázár, G., Kovács, Z., Kunisada, M., Morita, H., Kizaki, S., Sugiyama, H., Tsenkova, R. N., & Nishigori, C. (2015) · Scientific Reports · doi:10.1038/srep11808 · Cited by 56 · see citing works
DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing
Calses, P. C., Dhillon, K. K., Tucker, N., Chi, Y., Huang, J., Kawasumi, M., Nghiem, P., Wang, Y., Clurman, B. E., Jacquemont, C. F., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.03.021 · Cited by 44 · see citing works
Fort-Gallifa, I., García-Heredia, A., Hernández-Aguilera, A., Simó, J. M., Sepulveda, J. N., Martín‐Paredero, V., Camps, J., & Joven, J. (2016) · Free Radical Biology & Medicine · doi:10.1016/j.freeradbiomed.2016.07.011 · Cited by 39 · see citing works
Auclair, Y., Rouget, R., Belisle, J. M., Costantino, S., & Drobetsky, E. A. (2010) · DNA Repair · doi:10.1016/j.dnarep.2010.03.013 · Cited by 34 · see citing works
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Jabeen, G., Ahmad, M. H., Aslam, M., Riaz, S., Hayat, A., & Nawaz, M. H. (2022) · RSC Advances · doi:10.1039/d2ra04462e · Cited by 25 · see citing works
Musich, P. R., Li, Z., & Zou, Y. (2017) · Advances in Experimental Medicine and Biology · doi:10.1007/978-3-319-56017-5_4 · Cited by 23 · see citing works
The Functions of Serine 687 Phosphorylation of Human DNA Polymerase η in UV Damage Tolerance
Dai, X., You, C., & Wang, Y. (2016) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m115.052167 · Cited by 19 · see citing works
AIF promotes a JNK1-mediated cadherin switch independently of respiratory chain stabilization
Scott, A. J., Walker, S. A., Krank, J. J., Wilkinson, A. S., Johnson, K. M., Lewis, E. M., & Wilkinson, J. C. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.004022 · Cited by 13 · see citing works
Latimer, J. J., & Kelly, C. M. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-739-6_36 · Cited by 12 · see citing works
EZH2 suppresses the nucleotide excision repair in nasopharyngeal carcinoma by silencing XPA gene
Huang, Y. J., Wang, X., Niu, X., Wang, X., Jiang, R., Xu, T., Liu, Y., Liang, L., Ou, X., Xing, X., et al. (2016) · Molecular Carcinogenesis · doi:10.1002/mc.22507 · Cited by 12 · see citing works
Xu, D., Cao, Q., Wang, L., Wang, J., Xu, B., Attwood, K., Wei, L., Wu, Y., Smith, G. J., Katsuta, E., et al. (2022) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-21-0613 · Cited by 12 · see citing works
Prósz, A. G., Duan, H., Tisza, V., Sahgal, P., Topka, S., Klus, G. T., Börcsök, J., Sztupinszki, Z. M., Hanlon, T., Dióssy, M., et al. (2023) · Scientific Reports · doi:10.1038/s41598-023-47946-4 · Cited by 10 · see citing works
Visual detection of cyclobutane pyrimidine dimer DNA damage lesions by Hg2+ and carbon dots
Singh, S., Singh, M. K., & Das, P. (2018) · Analytica Chimica Acta · doi:10.1016/j.aca.2018.02.029 · Cited by 8 · see citing works
Prósz, A. G., Sahgal, P., Huffman, B. M., Sztupinszki, Z. M., Morris, C. X., Chen, D. J., Börcsök, J., Dióssy, M., Tisza, V., Spisák, S., et al. (2024) · npj Precision Oncology · doi:10.1038/s41698-024-00561-6 · Cited by 9 · see citing works
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Nakano, E., Takeuchi, S., Ono, R., Tsujimoto, M., Masaki, T., & Nishigori, C. (2017) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2017.08.046 · Cited by 6 · see citing works
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Kaminski, R., Cheeseboro, L., Amini, S., Johnson, E. M., White, M. K., Khalili, K., & Darbinyan, A. (2010) · Cell Cycle · doi:10.4161/cc.9.20.13456 · Cited by 5 · see citing works
Pimpley, M. R., Foley, M. L., & Latimer, J. J. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0223-2_27 · Cited by 5 · see citing works
Epidermal Pigmentation, Nucleotide Excision Repair and Risk of Skin Cancer
(2012) · Journal of Carcinogenesis & Mutagenesis · doi:10.4172/2157-2518.s4-001 · Cited by 4 · see citing works
Immuno-capture of UVDE generated 3’-OH ends at UV photoproducts
Peyresaubes, F., D’Amours, A., Leduc, F., Grégoire, M.-C., Boissonneault, G., & Conconi, A. (2015) · DNA repair · doi:10.1016/j.dnarep.2015.09.019 · Cited by 2 · see citing works
P53 and Thymidine Dimer Induction in Daily Low Emission Broad Band UV Treatment
Franken, S. M. (2016) · MOJ Immunology · doi:10.15406/moji.2016.04.00123 · Cited by 2 · see citing works
Cai, Q. (2014) · eScholarship (California Digital Library)
Hypoxia Suppresses DNA Repair: Implications for Cancer Progression and Treatment
Chan, N. H. L. (2011) · TSpace (University of Toronto)
Scott, A. J. (2019) · NDSU Repository (North Dakota State University)
Xu, D., Cao, Q., Wang, L., Wang, J., Xu, B., Attwood, K. M., Wei, L., Wu, Y., Smith, G. J., Katsuta, E., et al. (2023) · doi:10.1158/1535-7163.c.6543430
Régulation dépendante du cycle cellulaire de la réparation par excision de nucléotides
Auclair, Y. (2009) · doi:10.71781/28500
Self-citation
- Authors
- Rouget, R., Auclair, Y., Loignon, M., Affar, E. B., & Drobetsky, E. A.
- Affiliations
- Hôpital Maisonneuve-Rosemont (CA)
- Corresponding author
- Drobetsky, Elliot A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2008-02
- Volume
- 283
- Issue
- 9
- Pages
- 5533-5541
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1995828394
- Cited by
- 30
- References
- 44
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 2 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 3 · 2012: 1
- FWCI
- 0.66
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Keywords
- nucleotide excision repair, MAPK signaling, UV-induced DNA damage, ERK1/2, JNK1/2, p38 MAPK, MAPK inhibitors, shRNA knockdown, clonogenic survival, apoptosis, cell proliferation, dermal fibroblasts, lung fibroblasts, colon cancer cells, osteosarcoma cells
- MeSH terms
- Cell Survival, DNA Damage, DNA Repair, Flow Cytometry, Humans, Phosphorylation, Ultraviolet Rays, Genome, Human, Apoptosis, DNA Adducts, Mitogen-Activated Protein Kinase 1, MAP Kinase Signaling System, Cell Line, Tumor, Protein Kinase Inhibitors, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinase 8, Mitogen-Activated Protein Kinase 9, Mitogen-Activated Protein Kinase 11, Cell Proliferation
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