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Specificity of Metal Sensing: Iron and Manganese Homeostasis in Bacillus subtilis

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Helmann, John D.·2014·Journal of Biological Chemistry
DOI 10.1074/jbc.r114.587071PMC PMC4192466
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Abstract

Metalloregulatory proteins allow cells to sense metal ions and appropriately adjust the expression of metal uptake, storage, and efflux pathways. Bacillus subtilis provides a model for the coordinate regulation of iron and manganese homeostasis that involves three key regulators: Fur senses iron sufficiency, MntR senses manganese sufficiency, and PerR senses the intracellular Fe/Mn ratio. Here, I review the structural and physiological bases of selective metal perception, the effects of non-cognate metals, and mechanisms that may serve to coordinate iron and manganese homeostasis.

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Crossref · 10.1074/jbc.r114.587071

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  • Fe2+ is the dominant oxidation state in the labile iron pool.

    “The transition metals Mn2+ and Fe2+ (the dominant oxidation state in the labile pool) have a moderate affinity for ligands and cells have lower quotas (often ∼0.1 to 1 mM).1,10,11”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [10]

    The copy discusses Fe2+ as a metal that can form part of the labile pool and, under “Metal Ion Speciation in the Cell” (indexed as Abstract; no page given), says oxidized iron stored in ferritins no longer contributes to that pool. It does not state that Fe2+ is the dominant oxidation state within the labile iron pool. (LLM, conf=0.83)

    Experimental — accuracy not yet measured

  • The transition metals Mn2+ and Fe2+ have a moderate affinity for ligands.

    “The transition metals Mn2+ and Fe2+ (the dominant oxidation state in the labile pool) have a moderate affinity for ligands and cells have lower quotas (often ∼0.1 to 1 mM).1,10,11”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [10]

  • Cells have lower quotas for Mn and Fe, often ∼0.1 to 1 mM.

    “The transition metals Mn2+ and Fe2+ (the dominant oxidation state in the labile pool) have a moderate affinity for ligands and cells have lower quotas (often ∼0.1 to 1 mM).1,10,11”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [10]

  • B. subtilis has a more balanced demand for manganese and iron.

    “Other organisms, such as B. subtilis, have a more balanced demand for manganese and iron.10,91”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [10]

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Authors
Helmann, J. D.
Affiliations
Cornell University (US)
Corresponding author
Helmann, John D.
ORCID
Helmann, John D.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2014-10
Volume
289
Issue
41
Pages
28112-28120
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.r114.587071
PMC
PMC4192466
OpenAlex
W2027602425
Cited by
153
References
9
Citations by year
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FWCI
2.07
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (GM059323)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
metalloregulatory proteins, iron homeostasis, manganese homeostasis, Bacillus subtilis, Fur protein, Mn/Fe ratio
MeSH terms
Bacillus subtilis, Bacterial Proteins, Cations, Divalent, Homeostasis, Iron, Manganese, Models, Molecular, Repressor Proteins, Species Specificity, Structure-Activity Relationship, Gene Expression Regulation, Bacterial

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