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Antioxidant Peptidomics Reveals Novel Skin Antioxidant System
Open accessAbstract
It is generally agreed that reactive oxygen species (ROS) contribute to skin aging, skin disorders, and skin diseases. Skin possesses an extremely efficient antioxidant system. This antioxidant activity is conferred by two systems: antioxidant enzymes and small molecules that can scavenge ROS by donating electrons. No gene-encoded secreted ROS scavengers have been reported. Amphibian skin is a multifunctional organ acting in defense, respiration, and water regulation, although it seems susceptible. Amphibian skins are easily harmed by biological or non-biological attacks such as microorganism infection or radiation injury. Among vertebrates, skins of amphibian are exposed to more dangers of radiation injury than others. Radiation toxicity occurs by directly attacking the genetic material and/or by generating ROS. In addition, amphibian skin respiration and inflammatory response also induce ROS generation. It is rational to hypothesize that amphibian skins should have potent free radical scavenging and radioprotective ability for their survival. Rana pleuraden is distributed in Southwest of China; it lives in the subtropical plateau (altitude around 2300 m) where there is strong ultraviolet radiation and long duration of sunshine. By peptidomics and genomics approaches, a large amount of antioxidant peptides belonging to 11 different groups with variable structures were isolated from the skin secretions of R. pleuraden. Their free radical scavenging and anti-inflammatory abilities were studied. All of these peptide share highly homologous preproregions, although mature antioxidant peptides have very divergent primary structures, suggesting the possibility of a common ancestor. Some peptides were also found to have multifunctional properties, such as combined antioxidant, anti-inflammatory, and antimicrobial activities. According to our knowledge, no gene-encoded specific antioxidant peptides have been reported except metallothionein. Our work possibly reveals a new skin antioxidant system. The current work also provides a large amount of peptide candidates with medical-pharmaceutical significance.
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“They possess gene-encoded origins as antioxidant enzymes do, but they show no enzyme activity. Instead they function as direct free radical scavengers like LMWAs.”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Yang et al., 2009
In the citing paper
“The AOPs can rapidly and constantly eliminate the free radicals of ABTS+ and/or DPPH that generated by the commercial radical initiators in vitro (Yang et al., 2009; Liu et al., 2010; Lu et al., 2010; Yu et al., 2015; Niu et al., 2018; Cao et al., 2019).”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Yang et al., 2009
In the citing paper
“series of peptides have been identified with antioxidant activity from the skin of frogs lived in this plateau area (Yang et al., 2009; Liu et al., 2010)”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Yang et al., 2009
In the citing paper
“the previous studies did not investigate whether these peptides have anti-photoaging activity”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Yang et al., 2009
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Cites
Antioxidant activity applying an improved ABTS radical cation decolorization assay
Re, R., Pellegrini, N., Proteggente, A. R., Pannala, A. S., Yang, M. H., & Rice-Evans, C. A. (1999) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00315-3 · Cited by 26,986
Colowick, S. P., Joshi, M. D., Jagannathan, & KAPLAN, N. O. (1966) · Cited by 26,137
Free radicals in biology and medicine
Gutteridge, J. M. C. (1991) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(91)90478-o · Cited by 17,178
NMRPipe: A multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G. W., Zhu, G., Pfeifer, J., & Bax, A. (1995) · Journal of Biomolecular NMR · doi:10.1007/bf00197809 · Cited by 15,961
Antimicrobial peptides of multicellular organisms
Zasloff, M. (2002) · Nature · doi:10.1038/415389a · Cited by 8,457
Oyaizu, M. (1986) · The Japanese Journal of Nutrition and Dietetics · doi:10.5264/eiyogakuzashi.44.307 · Cited by 7,826
Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?
Brogden, K. A. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1098 · Cited by 5,829
Matsudaira, P. T. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61070-1 · Cited by 4,951
Free Radicals in Biology and Medicine
Land, E. J. (1990) · International Journal of Radiation Biology · doi:10.1080/09553009014552071 · Cited by 4,871
Antioxidant and radical scavenging properties of curcumin
Ak, T. P., & Gülçın, İ. (2008) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2008.05.003 · Cited by 1,942
Conlon, J. M., Kolodziejek, J., & Nowotny, N. R. (2003) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2003.09.004 · Cited by 398
Clarke, B. T. (1997) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1017/s0006323197005045 · Cited by 393
Krishnan, N., Dickman, M. B., & Becker, D. F. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2007.10.054 · Cited by 391
Shindo, Y., Witt, E., & Packer, L. (1993) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12469048 · Cited by 363
Skin antioxidants: Their role in aging and in oxidative stress — New approaches for their evaluation
Kohen, R. (1999) · Biomedicine & Pharmacotherapy · doi:10.1016/s0753-3322(99)80087-0 · Cited by 260
Anti-infection Peptidomics of Amphibian Skin
Li, J., Xu, X., Xu, C., Zhou, W., Zhang, K., Yu, H., Zhang, Y., Zheng, Y., Rees, H. H., Lai, R., et al. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m600334-mcp200 · Cited by 224
Self-citationThe Importance of Proteins in Defense Against Oxidation
Bourdon, E., & Blache, D. (2001) · Antioxidants & Redox Signaling · doi:10.1089/152308601300185241 · Cited by 220
Antimicrobial peptides from skin secretions of Chinese red belly toad Bombina maxima
Lai, R., Zheng, Y., Shen, J.-H., Liu, G.-J., Liu, H., Lee, W., Tang, S.-Z., & Zhang, Y. (2002) · Peptides · doi:10.1016/s0196-9781(01)00641-6 · Cited by 212
Self-citationSkin low molecular weight antioxidants and their role in aging and in oxidative stress
Kohen, R., & Gati, I. (2000) · Toxicology · doi:10.1016/s0300-483x(00)00206-7 · Cited by 197
Evaluation of antioxidant activity of Cassia siamea flowers
Kaur, G., Alam, M. S., Jabbar, Z., Javed, K., & Athar, M. (2006) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2006.05.021 · Cited by 196
Duda, T. F., Vanhoye, D., & Nicolas, P. (2002) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a004143 · Cited by 173
Evaluation of Plasma Low Molecular Weight Antioxidant Capacity by Cyclic Voltammetry
Chevion, S., Berry, E. M., Kitrossky, N., & Kohen, R. (1997) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(96)00337-1 · Cited by 148
Portugal, M., Barak, V., Ginsburg, I., & Kohen, R. (2007) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2007.05.010 · Cited by 121
The Lipocalin α1-Microglobulin Has Radical Scavenging Activity
Åkerström, B., Maghzal, G. J., Winterbourn, C. C., & Kettle, A. J. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m702624200 · Cited by 119
Free radical reduction in the human epidermis
Schallreuter, K. U., & Wood, J. M. (1989) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(89)90045-2 · Cited by 117
Xu, X., Yang, H., Ma, D., Wu, J., Wang, Y., Song, Y., Wang, X., Lu, Y., Yang, J., & Lai, R. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m700497-mcp200 · Cited by 93
Self-citationKohen, R., Beit‐Yannai, E., Berry, E. M., & Tirosh, O. (1999) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(99)00135-4 · Cited by 91
Li, J., Zhang, C., Xu, X., Wang, J., Yu, H., Lai, R., & Gong, W. (2007) · The FASEB Journal · doi:10.1096/fj.06-7966com · Cited by 72
Self-citationMultiple Bradykinin-Related Peptides From the Skin of the Frog, Rana temporaria
Conlon, J. M., & Aronsson, U. (1997) · Peptides · doi:10.1016/s0196-9781(96)00339-7 · Cited by 68
Lü, Y., Li, J., Yu, H., Xu, X., Liang, J., Tian, Y., Ma, D., Lin, G., Huang, G., & Lai, R. (2006) · Peptides · doi:10.1016/j.peptides.2006.08.017 · Cited by 64
Self-citationLai, R., Liu, H., Lee, W. H., & Zhang, Y. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.5359 · Cited by 64
Self-citationPurification and characterization of bioactive peptides from skin extracts of Rana esculenta
Simmaco, M., Biase, D. D., Severini, C., Aita, M., Erspamer, G. F., Barra, D., & Bossa, F. (1990) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(90)90140-r · Cited by 62
Lee, W., Li, Y., Lai, R., Li, S., Zhang, Y., & Wang, W. (2005) · European Journal of Immunology · doi:10.1002/eji.200425615 · Cited by 61
Self-citationRe-evaluation of the Antioxidant Prenylated Flavonoids from the Roots of Sophora flavescens
Jung, H. A., Jeong, D. M., Chung, H. Y., Lim, H. A., Kim, J., Yoon, N. Y., & Choi, J. S. (2008) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.31.908 · Cited by 58
Reducing equivalents in the aging process
Kohen, R., Fanberstein, D., & Tirosh, O. (1997) · Archives of Gerontology and Geriatrics · doi:10.1016/s0167-4943(96)00744-3 · Cited by 54
von Gadow, A., Joubert, E., & Hansmann, C. F. (1997) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf960280v · Cited by 51
A new family of antimicrobial peptides from skin secretions of Rana pleuraden
Wang, X., Song, Y., Li, J., Liu, H., Xu, X., Lai, R., & Zhang, K. (2007) · Peptides · doi:10.1016/j.peptides.2007.07.020 · Cited by 42
Self-citationAmphibian tachykinin precursor
Li, J., Liu, T., Xu, X., Wang, X., Wu, M., Yang, H., & Lai, R. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.09.150 · Cited by 31
Self-citationZhou, M., Chen, T., Walker, B., & Shaw, C. (2006) · Peptides · doi:10.1016/j.peptides.2006.04.007 · Cited by 31
Isolation and cDNA cloning of cholecystokinin from the skin of Rana nigrovittata
Liu, X., Wang, Y., Cheng, L., Song, Y., & Lai, R. (2007) · Peptides · doi:10.1016/j.peptides.2007.07.006 · Cited by 26
Self-citationMetallothionein expression by NG2 glial cells following CNS injury
Chung, R. S., Fung, S. J., Leung, Y. K., Walker, A. K., McCormack, G. H., Chuah, M. I., Vickers, J. C., & West, A. K. (2007) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-7267-8 · Cited by 13
Research works printed but not linked: 2
- Rieger, 1993, Oxidative reactions in and on the skin: mechanism and prevention, Cosmet. Toiletries, 108, 43
- Kohen, 1996, Evaluation of the total low molecular weight antioxidant activity of plasma in health and diseases: a new approach, Cell. Pharmacol., 3, 355
Cited by
Sorted by citations
The Chemistry and Biological Activities of Peptides from Amphibian Skin Secretions
Xu, X., & Lai, R. (2015) · Chemical Reviews · doi:10.1021/cr4006704 · Cited by 338
Self-citationDallas, D. C., Guerrero, A., Parker, E. A., Robinson, R. C., Gan, J., German, J. B., Barile, D., & Lebrilla, C. B. (2014) · PROTEOMICS · doi:10.1002/pmic.201400310 · Cited by 257
Antioxidant peptides, the guardian of life from oxidative stress
Zhu, Y., Wang, K., Jia, X., Fu, C., Yu, H., & Wang, Y. (2023) · Medicinal Research Reviews · doi:10.1002/med.21986 · Cited by 198
Self-citationThe diversity and evolution of anuran skin peptides
König, E., Bininda‐Emonds, O. R. P., & Shaw, C. (2014) · Peptides · doi:10.1016/j.peptides.2014.11.003 · Cited by 167
Antimicrobial Peptides and Copper(II) Ions: Novel Therapeutic Opportunities
Portelinha, J., Duay, S. S., Yu, S. I., Heilemann, K., Libardo, M. D. J., Juliano, S. A., Klassen, J. L., & Angeles-Boza, A. M. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00921 · Cited by 112
Metadata
- Authors
- Yang, H., Wang, X., Liu, X., Wu, J., Liu, C., Gong, W., Zhao, Z., Hong, J., Lin, D., Wang, Y., & Lai, R.
- Affiliations
- Kunming Institute of Zoology (CN)
- Nanjing Agricultural University (CN)
- Chinese Academy of Sciences (CN)
- Zhejiang University (CN)
- Corresponding author
- Lai, Ren
- ORCID
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2009-03
- Volume
- 8
- Issue
- 3
- Pages
- 571-583
- ISSN
- 1535-9476 (ISSN-L)
- PMC
- PMC2649818
- OpenAlex
- W2074935079
- Cited by
- 156
- References
- 44
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 9 · 2023: 7 · 2022: 11 · 2021: 13 · 2020: 9 · 2019: 14 · 2018: 9 · 2017: 13 · 2016: 6 · 2015: 9 · 2014: 8 · 2013: 10 · 2012: 7
- FWCI
- 5.12
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular & Cellular Proteomics (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chinese Academy of Sciences
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Venomous Animal Envenomation and Studies
- Keywords
- antioxidant peptides, amphibian skin secretions, reactive oxygen species, free radical scavenging, anti-inflammatory activity, antimicrobial peptides, radiation protection, ultraviolet radiation, skin aging
- MeSH terms
- Alkylation, Amino Acid Sequence, Animals, Anti-Infective Agents, Anti-Inflammatory Agents, Antioxidants, Bacteria, Biphenyl Compounds, Fungi, Kinetics, Microbial Sensitivity Tests, Molecular Sequence Data, Magnetic Resonance Spectroscopy, Oxidation-Reduction, Peptides, Picrates, Ranidae, Skin, Free Radical Scavengers, Protein Sorting Signals, Antimicrobial Cationic Peptides, Proteomics
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