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Antioxidant Peptidomics Reveals Novel Skin Antioxidant System
Open accessAbstract
It is generally agreed that reactive oxygen species (ROS) contribute to skin aging, skin disorders, and skin diseases. Skin possesses an extremely efficient antioxidant system. This antioxidant activity is conferred by two systems: antioxidant enzymes and small molecules that can scavenge ROS by donating electrons. No gene-encoded secreted ROS scavengers have been reported. Amphibian skin is a multifunctional organ acting in defense, respiration, and water regulation, although it seems susceptible. Amphibian skins are easily harmed by biological or non-biological attacks such as microorganism infection or radiation injury. Among vertebrates, skins of amphibian are exposed to more dangers of radiation injury than others. Radiation toxicity occurs by directly attacking the genetic material and/or by generating ROS. In addition, amphibian skin respiration and inflammatory response also induce ROS generation. It is rational to hypothesize that amphibian skins should have potent free radical scavenging and radioprotective ability for their survival. Rana pleuraden is distributed in Southwest of China; it lives in the subtropical plateau (altitude around 2300 m) where there is strong ultraviolet radiation and long duration of sunshine. By peptidomics and genomics approaches, a large amount of antioxidant peptides belonging to 11 different groups with variable structures were isolated from the skin secretions of R. pleuraden. Their free radical scavenging and anti-inflammatory abilities were studied. All of these peptide share highly homologous preproregions, although mature antioxidant peptides have very divergent primary structures, suggesting the possibility of a common ancestor. Some peptides were also found to have multifunctional properties, such as combined antioxidant, anti-inflammatory, and antimicrobial activities. According to our knowledge, no gene-encoded specific antioxidant peptides have been reported except metallothionein. Our work possibly reveals a new skin antioxidant system. The current work also provides a large amount of peptide candidates with medical-pharmaceutical significance.
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“They possess gene-encoded origins as antioxidant enzymes do, but they show no enzyme activity. Instead they function as direct free radical scavengers like LMWAs.”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Yang et al., 2009
In the citing paper
“The AOPs can rapidly and constantly eliminate the free radicals of ABTS+ and/or DPPH that generated by the commercial radical initiators in vitro (Yang et al., 2009; Liu et al., 2010; Lu et al., 2010; Yu et al., 2015; Niu et al., 2018; Cao et al., 2019).”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Yang et al., 2009
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“series of peptides have been identified with antioxidant activity from the skin of frogs lived in this plateau area (Yang et al., 2009; Liu et al., 2010)”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Yang et al., 2009
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“the previous studies did not investigate whether these peptides have anti-photoaging activity”
From A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species (2022) · cites this paper as Yang et al., 2009
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Cited by
Sorted by citations
The Chemistry and Biological Activities of Peptides from Amphibian Skin Secretions
Xu, X., & Lai, R. (2015) · Chemical Reviews · doi:10.1021/cr4006704 · Cited by 338
Self-citationDallas, D. C., Guerrero, A., Parker, E. A., Robinson, R. C., Gan, J., German, J. B., Barile, D., & Lebrilla, C. B. (2014) · PROTEOMICS · doi:10.1002/pmic.201400310 · Cited by 257
Antioxidant peptides, the guardian of life from oxidative stress
Zhu, Y., Wang, K., Jia, X., Fu, C., Yu, H., & Wang, Y. (2023) · Medicinal Research Reviews · doi:10.1002/med.21986 · Cited by 198
Self-citationThe diversity and evolution of anuran skin peptides
König, E., Bininda‐Emonds, O. R. P., & Shaw, C. (2014) · Peptides · doi:10.1016/j.peptides.2014.11.003 · Cited by 167
Antimicrobial Peptides and Copper(II) Ions: Novel Therapeutic Opportunities
Portelinha, J., Duay, S. S., Yu, S. I., Heilemann, K., Libardo, M. D. J., Juliano, S. A., Klassen, J. L., & Angeles-Boza, A. M. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00921 · Cited by 112
Metadata
- Authors
- Yang, H., Wang, X., Liu, X., Wu, J., Liu, C., Gong, W., Zhao, Z., Hong, J., Lin, D., Wang, Y., & Lai, R.
- Affiliations
- Kunming Institute of Zoology (CN)
- Nanjing Agricultural University (CN)
- Chinese Academy of Sciences (CN)
- Zhejiang University (CN)
- Corresponding author
- Lai, Ren
- ORCID
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2009-03
- Volume
- 8
- Issue
- 3
- Pages
- 571-583
- ISSN
- 1535-9476 (ISSN-L)
- PMC
- PMC2649818
- OpenAlex
- W2074935079
- Cited by
- 156
- References
- 44
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 9 · 2023: 7 · 2022: 11 · 2021: 13 · 2020: 9 · 2019: 14 · 2018: 9 · 2017: 13 · 2016: 6 · 2015: 9 · 2014: 8 · 2013: 10 · 2012: 7
- FWCI
- 5.12
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular & Cellular Proteomics (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chinese Academy of Sciences
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Venomous Animal Envenomation and Studies
- Keywords
- antioxidant peptides, amphibian skin secretions, reactive oxygen species, free radical scavenging, anti-inflammatory activity, antimicrobial peptides, radiation protection, ultraviolet radiation, skin aging
- MeSH terms
- Alkylation, Amino Acid Sequence, Animals, Anti-Infective Agents, Anti-Inflammatory Agents, Antioxidants, Bacteria, Biphenyl Compounds, Fungi, Kinetics, Microbial Sensitivity Tests, Molecular Sequence Data, Magnetic Resonance Spectroscopy, Oxidation-Reduction, Peptides, Picrates, Ranidae, Skin, Free Radical Scavengers, Protein Sorting Signals, Antimicrobial Cationic Peptides, Proteomics
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