The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Refined Preparation and Use of Anti-diglycine Remnant (K-ε-GG) Antibody Enables Routine Quantification of 10,000s of Ubiquitination Sites in Single Proteomics Experiments
Open accessAbstract
Detection of endogenous ubiquitination sites by mass spectrometry has dramatically improved with the commercialization of anti-di-glycine remnant (K-ε-GG) antibodies. Here, we describe a number of improvements to the K-ε-GG enrichment workflow, including optimized antibody and peptide input requirements, antibody cross-linking, and improved off-line fractionation prior to enrichment. This refined and practical workflow enables routine identification and quantification of ∼20,000 distinct endogenous ubiquitination sites in a single SILAC experiment using moderate amounts of protein input.
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Cox, J., & Mann, M. (2008) · Nature Biotechnology · doi:10.1038/nbt.1511 · Cited by 14,855
Smyth, G. K. (2004) · Statistical Applications in Genetics and Molecular Biology · doi:10.2202/1544-6115.1027 · Cited by 11,829
Ong, S.-E., Blagoev, B., Kratchmarova, I., Kristensen, D. B., Steen, H., Pandey, A., & Mann, M. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m200025-mcp200 · Cited by 5,574
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Self-citationLarge-scale identification of ubiquitination sites by mass spectrometry
Udeshi, N. D., Mertins, P., Svinkina, T., & Carr, S. A. (2013) · Nature Protocols · doi:10.1038/nprot.2013.120 · Cited by 296
Self-citationMass Spectrometry-Based Detection and Assignment of Protein Posttranslational Modifications
Doll, S., & Burlingame, A. L. (2015) · ACS Chemical Biology · doi:10.1021/cb500904b · Cited by 261
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Self-citationA lysosomal enigma CLN5 and its significance in understanding neuronal ceroid lipofuscinosis
Basak, I., Wicky, H. E., McDonald, K. O., Xu, J. B., Palmer, J. E., Best, H. L., Lefrancois, S., Lee, S. Y., Schoderboeck, L., & Hughes, S. M. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-021-03813-x · Cited by 32
Metadata
- Authors
- Udeshi, N. D., Svinkina, T., Mertins, P., Kuhn, E., Mani, D. R., Qiao, J. W., & Carr, S. A.
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2013-03
- Volume
- 12
- Issue
- 3
- Pages
- 825-831
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2118453371
- Cited by
- 333
- References
- 3
- FWCI
- 5.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Udeshi, N. D., Svinkina, T., Mertins, P., Kuhn, E., Mani, D. R., Qiao, J. W., & Carr, S. A. (2013). Refined Preparation and Use of Anti-diglycine Remnant (K-ε-GG) Antibody Enables Routine Quantification of 10,000s of Ubiquitination Sites in Single Proteomics Experiments. Molecular & Cellular Proteomics. https://doi.org/10.1074/mcp.o112.027094
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